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Showing 1 to 10 of 10 for “"Glycoside Hydrolases"”.
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Molecular Design and Mechanistic Characterization of Glycoside Hydrolases using Computational and Experimental Techniques
… enzymes, including endoglucanases, cellobiohydrolases and glucosidases that work synergistically to solubilize crystalline cellulose efficiently. The dependence of hydrolysis reaction rate on temperature predicts that large increases in performance and decreased enzyme cost would be achieved …
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Bioprospecting the Regional Diversity of Australian Wine Microbiota
… of many wine compounds. Of these enzymes, glycoside hydrolases mediate hydrolysis of glycosides and are found ubiquitously through nature. Micro-organisms, especially those associated with plants, use glycoside hydrolases to afford access to plant resources by degrading the plant cell wall …
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Biochemical and molecular analyses of plant cell wall degrading enzymes from the rumen bacterium Ruminococcus albus 8
… of R. albus 8 revealed five genes encoding glycoside hydrolases predicted to hydrolyze the xylose backbone and several genes encoding putative accessory enzymes that are expected to remove side chains from xylans. The genes were cloned, heterologously expressed in E. coli, and biochemically …
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Microbial glucosinolate metabolism: Characterization of Enterococcus faecalis XL1 and functional analysis of a GH4 enzyme
… phosphotransferase system (PTS) transporters and glycoside hydrolases (GHs) responsive to sinigrin, with a GH4 enzyme Ef5510, showing the strongest induction. Functional studies revealed that Ef5510 is an NAD+/Mn2+-dependent hydrolase, and the PTS-mediated uptake step represents the rate-limiting …
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Investigating and exploiting fungal natural products in Aspergillus spp.
… the abundance of hydrolytic enzymes, including glycoside hydrolases (n = 22) and peptidases (n = 16) which have important applications in the biotechnology industry. A significant dysregulation of proteins involved in amino acid metabolism coupled to an increase in the levels of selected amino …
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Functional Genomic Examinations Of Interactions Between Common Members Of The Human Gut Microbiota
… vulgatus,</italic> upregulates its unique glycoside hydrolase activities to adapt to the presence of <italic>E. rectale,</italic> increasing its degradation of host-derived glycans that <italic>E. rectale</italic> cannot use. In contrast, <italic>E. rectale</italic> downregulates its …
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Investigation of the role of the extracellular β-agarase, produced by the bacterial epiphyte Pseudoalteromonas sp. LS2i, in the virulence response towards the agarophyte Gracilaria gracilis
… identified as a member of the GH-16 family of glycoside hydrolases. The agarase was purified from the E. coli JM109 (pEB3) transformant. The molecular weight was estimated to be 39 kDa by SDS-PAGE. Zymogram analysis confirmed that the purified protein is agarolytic and TLC analysis revealed …
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Molecular mechanisms behind the adjustment of phototrophic light-harvesting and mixotrophic utilization of cellulosic carbon sources in Chlamydomonas reinhardtii
… revealed that it contains genes encoding glycoside hydrolases of different families, known to be involved in cellulose- and hemicellulose degradation, even though the cell wall of this alga does not contain cellulose and is solely composed of hydroxyproline-rich glycoproteins. This work …
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Purification and characterization of fungal β-glucosidase enzymes
… end-product inhibition. β-Glucosidases belong to Glycoside hydrolases (GH), that can be classified into different protein families. In an industrial perspective, the main interest resides in GH1 and GH3 family enzymes. Many industrially relevant extracellular β-glucosidases belong to GH3 family. …
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Metagenomanalysen von zwei Habitaten mit (hemi-)cellulolytischen mikrobiellen Gemeinschaften
Die Analyse von Metagenomen ermöglicht nicht nur einen Einblick in die mikrobiellen Diversität eines Habitats, sie kann auch durch die Durchmusterung von Umweltgenbanken zu der Entdeckung von neuartigen, biotechnologisch anwendbaren Biokatalysatoren führen. In der vorliegenden Arbeit wurde zum …