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Showing 1 to 20 of 30 for “"Glycosidase"”.
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Characterisation of glycosidase enzymes of wine lactic acid bacteria
… in wine, attention has to focus on five glycosidase enzymes: β-D- glucopyranosidase, α-D-glucopyranosidase, α-L-rhamnopyranosidase, α-L arabinofuranosidase and β-D-xylopyranosidase. This project has investigated the presence and distribution of glycosidases amongst 40 isolates of Lactic …
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Diastereoselective acylation of trans-2-substituted-cyclohexanols and glycosidase inhibition studies
… were tested as inhibitors of fungal glycosidases. Two compounds showed greater than 80% inhibition values in excess of the activity of β-D-glucosidases. More interestingly, the same compounds showed a marked enhancement of α-D-galactosidase activity by as much as 35%.</p>
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Developing methods to construct ring pucker free energy hypersurfaces applied to the analysis of glycosidase enzyme catalytic mechanisms
… glucose subunit and is degraded by cellulase, a glycosidase. Specifically, the retaining cellobiohydrolase I (CBHI) of Trichoderma Reesei which cleaves cellobiose units from crystalline cellulose.The free energy volumes of puckering for the glucose sub-unit (in the catalytic position of an 8 unit …
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The individual and simultaneous effects of Cu and cypermethrin upon glycosidase, phosphomonoesterase and the total microbial activity of soil.
… a chemical disturbance. This study chose β- glycosidase, phosphomonoesterase and the total microbial activity of soil to establish the chemical disturbance of pyrethroid insecticide cypermethrin and CuSO4, the main chemical component of widely used Bordeaux mixture. Both were applied …
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The effects of post-fermentation and post-bottling heat treatment on Cabernet Sauvignon (V. vinifera L.) glycosides and quantification of glycosidase activities in selected strains of Brettanomyces bruxellensis and Oenococcus oeni
Thermal processing has been used as a means of modifying the sensory aspects of wine. Cabernet Sauvignon wines were heated prior to dejuicing (3C per day from 25C to 42C) or after bottling (42C for 21 days) to determine the effects on total glycosides and glycosidic fractions. Total and phenol-free …
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FROM AGRI-FOOD WASTE TO HIGH-VALUE COMPOUNDS VIA GREEN TECHNOLOGY APPROACHES
… commercial α-rhamnosidase and an extremophilic β-glycosidase was implemented for the efficient hydrolysis of citrus ru:nosyl flavonoids, achieving >99% conversion in con:nuous flow under zero-waste condi:ons. Second, soybean glycosides were converted into bioac:ve aglycones and lipophilized …
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The Total Synthesis of (-)-Detoxinine and (+)-Casuarine Using Tandem [4 + 2]/[3 + 2] Nitroalkene Cycloadditions and Cycloadditions of Nitroethylene
… of the six stereocenters present in this potent glycosidase inhibitor. The completion of the synthesis required only four additional steps to deliver the final product in 20% overall yield.
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The effects of different processing parameters (cold soak and percent alcohol (v/v) at dejuicing) on the concentrations of grape glycosides and glycoside fractions and glycosidase activities in selected yeast and lactic acid bacteria
Grape-derived aroma and flavor precursors exist partially as non-volatile, sugar-bound glycosides. Hydrolysis of these compounds may modify sensory attributes and potentially enhance wine quality. Cold soak (prefermentation skin contact) at two temperatures and alcohol content (%, v/v) at dejuicing …
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… synthetic preparation and structural analysis. Glycosidases and glycosyltransferases are important components of the glycobiology toolbox that facilitate manipulation of complex carbohydrate structures. Chapter 3 describes the in vitro selection of deoxyribozymes for O-glycoside cleavage. …
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Binding Determinants of HIV-1 REV RNA to the Pokeweed Antiviral Protein
The pokeweed antiviral protein (PAP) is an N-glycosidase that removes an adenine residue from the sarcin/ricin loop (SRL) of rRNA through a process called depurination. PAP has also been shown to depurinate the ORF of Rev RNA of HIV-1 in vivo without causing toxicity. The sequence and structure of …
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A stereoelectronic and thermodynamic study of b-D-methyl glucose conformational changes related to anomeric centre reactivity
… for ring deformation of -D-methyl glucose in glycosidase reactions (for example, cellulose hydrolysis). The investigation is computational and is done in isolation from the enzyme binding pocket and incoming nucleophile. What is the effect of the C1-O1 bond breaking process on key glucose …
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cis-Arenediols as versatile chiral synthons in the synthesis of prostaglandins, cyclitols, carbohydrates, and alkaloids
… in an approach to kifunensine (10), an important glycosidase inhibitor, which was approached according to the following retrosynthetic analysis: See: Figures 10, 11, 12, 5, 14, 13 A detailed study of the nucleophilic opening of epoxide 13 was carried out in order to better understand the …
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Novel methods for the isolation and purification of exoglycosidases
A number of exoglycosidases have been prepared from bacterial and plant sources using established methods for the separation of enzymes, in conjunction with certain novel purification systems hitherto not described in the literature for these enzymes. The enzyme, beta-galactosidase from E. coli has …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… protein (PAP) genes which belong to the N-glycosidase family. These proteins are called ribosome inactivating proteins (RIPs) which remove an adenine base from the sarcin/ricin loop (SRL) of the 28S rRNA in eukaryotes. This depurination results in protein translation inhibition leading to a …
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Untersuchungen zu Bacillus cereus Enterotoxin-Komplexen auf zellulärer Ebene
… nor enzymatic treatment with sialidase and N-glycosidase F resulted in an alteration of toxicity.
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ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΓΛΥΚΟΖΟΑΙΜΟΣΦΑΙΡΙΝΗΣ (CT- 6) ΚΑΙ ΤΗΣ ΚΑΜΠΥΛΗΣ ΓΛΥΚΟΖΗΣ (OGTT) ΣΕ ΦΥΣΙΟΛΟΓΙΚΕΣ ΚΑΙ ΠΑΘΟΛΟΓΙΚΕΣ ΚΑΤΑΣΤΑΣΕΙΣ
… values were observed after administration of a-glycosidase inhibitors, for a period of three weeks to 17 diabetics patients. Highly significant was the correlation of G-Hb and OGTT at the peak of the curve (r= + 0,83) up to the extend that glucose tolerance test may be replaced by G-Hb which has …
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Synthesis of Bulky and Polar Galactonoamidines for the Inhibition of the Human α-Galactosidase
<p>Glycosidases are amongst the most abundant enzymes in nature. This is due to their role in the degradation of carbohydrates which are the major source of carbon or earth. The absence or malfunction of glycosidases is implicated in numerous diseases such as cancers, diabetes, and lysosomal …
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Assays for lycosyltransferases
… acceptor and coupling of the GT reaction to a glycosidase reaction. During the development of this novel biochemical assay, unexpected enzymological features of LgtC were discovered. LC- MS/MS was used to investigate unknown products originating from the LgtC-catalysed reaction. The results …
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Cryptosporidium Parvum Microbial Adhesion: Interactions Between Sporozoites and Host Cells
… was resistant to heat, protease digestion, and glycosidase treatment, suggesting it may be lipid or a lipid-like component. Organic solvent extraction of MDBK cells and bovine mucosa resulted in recovery of inhibitory activity. Purification of these extracts by preparative thin-layer …
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