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Showing 1 to 20 of 26 for “"Glycogen phosphorylase"”.
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Mechanistic studies of rabbit muscle glycogen phosphorylase
Glycogen phosphorylase catalyzes the reversible phosphorolysis of glycogen. The enzyme contains a molecule of pyridoxal phosphate (PLP), of which the phosphate moiety is essential for catalysis. To investigate the role of the cofactor phosphate moiety two phosphonate analogues of PLP, …
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Regulation of glycogen phosphorylase genes in Dictyostelium discoideum
… crucial developmental event in Dictyostelium is glycogen degradation. The degradation of glycogen provides glucose monomers that are used to synthesize structural components necessary for cellular differentiation. Glycogen degradation is catalyzed by glycogen phosphorylase, and two …
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Molecular mechanism of glycogen phosphorylase gene regulation during Dictyostelium development
… transduction, and especially gene regulation. Glycogen phosphorylase is the enzyme that initiates one of the key biochemical pathways, glycogen degradation, during Dictyostelium discoideum development. Two forms of glycogen phosphorylase, gpl and gp2, exist in D. discoideum with gp1 being …
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The relationship between two forms of glycogen phosphorylase in Dictyostelium discoideum
… cell differentiation. In D. discoideum glycogen degradation provides precursors for the synthesis of developmentally regulated structural end products. The enzyme responsible for glycogen degradation, glycogen phosphorylase, exists in active and inactive forms. The activity of the ’a' …
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The relationship between the two forms of glycogen phosphorylase in Dictyostelium discoideum
… developmentally regulated forms of the enzyme glycogen phosphorylase. The inactive ’b’ form requires 5'AMP for activity and is present in early development, whereas the active ’a’ form is 5'AMP independent and is present in late development. Polyclonal antibodies raised to purified forms of the …
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Computer-aided design and synthesis of 5,7-dihydroxyflavone derivatives as glycogen phosphorylase inhibitors
… occurring flavonoids are known inhibitors of glycogen phosphorylase, which is a validated target for controlling hyperglycemia in type 2 diabetes. By exploiting computational methods such as molecular docking with Glide and post-docking binding free energy calculations using advanced …
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Transcriptional Regulation of the Glycogen Phosphorylase-2 Gene in <I>Dictyostelium discoideum</i>
The expression of the <I>glycogen phosphorylase- 2</I> gene (<I>gp2</I>) is initiated during early development and regulated by the extracellular morphogens cAMP and Differentiation Inducing Factor (DIF-1) [1-3]. Glycogen phosphorylase- 2 catalyzes the breakdown of glycogen reserves in developing …
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Footprint Analysis of the Transcriptional Control of Glycogen Phosphorylase 2 in Dictyostelium Discoideum
Glycogen phosphorylase 2 (gp-2) is a key enzyme during the development of Dictyostelium discoideum. The gp-2 enzyme breaks down glycogen into glucose monomers that are subsequently used to synthesize the terminal end products of cellular differentiation. This gene is an ideal candidate for studying …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… developmentally regulated forms of the enzyme glycogen phosphorylase, which are encoded by two distinct, but related genes (Rutherford, et. aI., 1991). A complementary DNA (cDNA) encoding glycogen phosphorylase-}, gp-l, was isolated from a λgtll expression library made from amoebae stage mRNA. …
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Function of a C-rich region in the transcriptional regulation of the glycogen phosphorylase-2 gene in Dictyostelium discoideum
The cellular slime mold Dictyostelium discoideum is a simple eukaryote that has been used as a model organism for the investigation of eukaryotic cell signaling, regulation of gene expression, and development. Transcription of the gp-2 gene is induced during development and is regulated by …
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The effects of fatigue on glycogen, glycogen phosphorylase, and calcium uptake associated with the sarcoplasmic reticulum of rat skeletal muscle
… relaxation. It has been proposed that both glycogen and glycolytic enzymes are associated with the SR membrane (SR-glycogenolytic complex). Interestingly, glycogen phosphorylase, an enzyme involved in glycogen breakdown, seems to be associated with the SR-glycogenolytic complex through its …
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A study of the regulation of glycogen metabolism in Dictyostelium discoideum
This work discusses the regulation of glycogen metabolism in Dictyostelium discoideum during its developmental cycle. Specifically, the possible cAMP dependent regulation of glycogen phosphorylase and glycogen synthase was examined. In other systems, cAMP can regulate at the level of the gene …
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Baculovirus-directed expression of the phosphorylase kinase catalytic subunit: pseudosubstrate and calmodulin regulation
Phosphorylase kinase (EC 2.7.1.38) is a key enzyme involved in the regulation of the glycogenolysis pathway. It catalyzes the Ca²⁺-dependent phosphorylation and activation of the enzyme glycogen phosphorylase to make the active form glycogen phosphorylase. Phosphorylase kinase is composed of 4 …
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Glycogen extraction from skeletal muscle sarcoplasmic reticulum: structural and functional implications
… were subjected to two different biochemical glycogen-extraction protocols. The results suggest that both amylase and removal of EDTA (No-EDTA) from the homogenization and storage buffers reduced the amount of glycogen associated with the SR. Both of these treatments failed to impair SR …
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Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum
… the life cycle of Dictyostelium discoideum is glycogen turnover. This process is catalyzed by glycogen phosphorylase-2 (gp-2). Since gp-2 expression is first induced during the transition from growth to differentiation, understanding how this gene is controlled may provide some insight into the …
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Purification of the proteins that bind to the gp-2 promoter in Dictyostelium discoideum
During <i>Dictyostelium</i> development, glycogen degradation is a crucial event that provides glucose monomers that are utilized to synthesize the essential structural components for cellular differentiation. The degradation is catalyzed by the product of the glycogen phosphorylase-2 gene. Cloning …
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An exploration of phosphorylases for the synthesis of carbohydrate polymers
Phosphorylases are interesting enzymes with regard to both their role in metabolism and their use in the in vitro synthesis of carbohydrates. The disaccharide phosphorylases have attracted attention because of their strict stereo- and regiospecificity and their tractability. The polymerising …
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
… in differentiation-related genes, including the glycogen phosphorylase 2 gene (gp2). Therefore, it is possible that DdRPA is not only a single-stranded DNA binding protein that is used in multiple essential DNA metabolic processes, such as DNA replication, repair and recombination in …
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Fuels for flight in the fruit beetle,Pachnoda Sinuata, and control of flight metabolism
… (glyceraldehyde-3-phosphatedehydrogenase, glycogen phosphorylase) were found to have high activities in flight muscles of P. sinuata, D. lunata and L. migratoria. Two methods of tethered flight were investigated. One of these allowed the animals to produce lift. During lift generating …
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Postmortem changes in meat quality and myofibrillar protein degradation in turkey breast muscle
… analyses revealed that the extractability of glycogen phosphorylase, creatine kinase, and M-protein was reduced for RG or DC samples. Increased myosin degradation was observed for samples from either RG or DC carcasses with up to 20% of myosin degraded in RG/DC samples. Nebulin degradation was …
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