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Showing 1 to 20 of 45 for “"Glycoconjugates"”.
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Synthesis Of Functionalized Gpi Anchors And Related Glycoconjugates
<p>This dissertation is divided into two chapters describing the background, significance, and synthesis of GPI anchors and GPI-anchored molecules. GPI anchors are a class of complex glycolipids with the primary purpose of attaching cell surface proteins/glycoproteins to the cell membrane. The …
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The Role of Glycoconjugates in Mediating Human Fertilization and Induction of Fetomaternal Tolerance
… sperm-egg binding assay, we show that specific glycoconjugates known to inhibit immune cell interactions mediated by the selectins, potently block human sperm-egg binding. The selectin ligand sialyl Lewis<sup>x</sup> inhibits sperm binding in the HZA by 60% at a concentration of 1 mg/ml. Our …
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The effects of vineyard management and primary and secondary fermentations on grape glycoconjugates and conjugate fractions
… to determine the effects on grape chemistry, glycoconjugates, and conjugate fractions. Reduced shoot density generally resulted in higher berry weight and lower soluble solids (°Brix) at each sampling date. Titratable acidity and pH were generally unaffected by shoot thinning. The 25 shoots …
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Preparation and Characterization of Carbohydrate-Functionalized Nanomaterials for Use as Cellular Probes and Targeted Delivery Vehicles
… reported herein details (1) the synthesis of glycoconjugates to functionalize the surface of gold, silica, nanoparticles, and surfactant vesicles, and (2) the preparation and characterization of carbohydrate-functionalized gold nanospheres and surfactant vesicles. A variety of N-linked …
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Synthesis of Novel Calix[4]arene Based Dendrimers and their Biological Applications
… 2 describes the development of calixarene glycoconjugates using a wide range of approaches. The synthesis of calixarene glycoconjugates through click chemistry is reported and the development of the first multicalixarene glycoconjugate is discussed. Chapter 3 reviews the development of …
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Proteomic analysis of the Mycobacterium avium-containing phagosome membrane
… phagosomes in terms of cell surface-derived glycoconjugates, revealed that these phagosomes show a 3- to 4-fold depletion of these glycoconjugates as compared to early endosomes with which the phagosomes continuously fuse and exchange membrane molecules... The aim of this study is to identify …
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I. Efforts Toward the Total Synthesis of the Stemofoline Alkaloids II. Preliminary Development of a Method to Prepare 1,2-Cis-2-Acetamido-2-Deoxyglycopyranoses
… method to prepare alpha-linked C2-acetamido glycoconjugates is described and preliminary results are presented.
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Development of novel conjugate vaccines against Salmonella
… protein. Several clinical trials have shown that glycoconjugates are promising vaccine candidates to prevent Salmonella infections. However, manufacturing of conjugate vaccines is a complex, multi-step process. An alternative approach to produce glycoconjugates is based on the bacterial N-linked …
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Solution and solid phase synthesis of N,N'-diacetyl chitotetraoses
… major biopolymers, proteins, nucleic acids and glycoconjugates are mainly responsible for the information transfer, which is a fundamental process of life. The biological importance of proteins and nucleic acids are well explored and oligosaccharides in the form of glycoconjugates have gained …
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Convergent Site-Selective Carbohydrate-Peptide Ligations With Dehydroalanine and Aziridine -2 -Carboxylic Acid-Containing Peptides
… of alpha-thio analogs of mucin related glycoconjugates is described. Chemoselectivity in these reactions was realized through the incorporation of dehydroalanine and aziridine-2-carboxylic acid residues into peptides and subsequent conjugation of the resulting peptide electrophiles with …
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NEU Insights and Development of Potential Therapeutics for Pulmonary Fibrosis
… neuraminidases) are enzymes that desialylate glycoconjugates by cleaving terminal sialic acids from the glycoconjugates. Our lab previously found that a sialylated serum glycoprotein called serum amyloid P (SAP) inhibits fibrosis, and that sialidases attenuate SAP function. Mammals have four …
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Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
… to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the …
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Evaluation of a gold nanoparticle platform as highly immunogenic and protective therapy against Burkholderia mallei, B. pseudomallei, and Enterohemorrhagic Escherichia coli O157:H7
… both glanders and melioidosis. In this study, glycoconjugates coupled to gold nanoparticles (AuNPs) were tested for their protective properties in clinically relevant infection models. The flexible NP platform allow us to incorporate novel antigens identified previously by a reverse vaccinology …
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Cryptosporidium Parvum Microbial Adhesion: Interactions Between Sporozoites and Host Cells
… described in natural infections. A variety of glycoconjugates, as well as plasma membrane vesicles, were screened for their ability to block C. parvum sporozoite binding to MDBK cells. Of the naturally occurring and synthetic glycoconjugates tested, only mucins inhibited binding between …
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Priming and processing glycosyltransferases in bacterial N-linked glycosylation pathways
… prominently feature a variety of complex glycoconjugates. Although these structures are very diverse, the biosynthesis of these glycoconjugates shares common themes. In particular, a priming glycosyltransferase or phosphoglycosyltransferase (PGT) initiates the biosynthesis of glycans by …
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Chemical synthesis of glycosylated oligoribonucleotides for siRNA development
… stability of RNA molecules was evaluated on two glycoconjugates, monomannosyl UlO-mer and dimannosyl UlO-mer. In the synthesis of aromatic fluorescent ribosides, perbenzylated ribofuranosyl pyrene and phenanthrene were synthesized from perbenzylated ribolactone. Deprotection of benzyl-protected …
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The kinetics of endosome processing
… galactose was covalently bound to cell-surface glycoconjugates on mouse macrophage-cells, P388D₁, as catalysed by galactosyltransferase. This provided a general membrane marker. After endocytosis-derived redistribution of membrane marker between the cell surface and endosomal membrane, a steady …
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Structural analysis of glycolipid-derived oligosaccharides from metabolically radiolabelled colorectal carcinoma SW1116 cells
… 1%-3% of the radioactivity into their glycoconjugates. Approximately 63% of the radioactivity recovered in the glycoconjugates corresponded to glycolipids when cells were labelled with 6-[³H]-D-galactose, and about 12% when cells were radiolabelled with 6- [³H]-D-glucosamine. …
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Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia Coli
… which can be found in the extracellular glycoconjugates of several bacterial pathogens. Due to the similarity in stereochemistry of the two compounds, legionaminic acid has great potential in the production of pharmaceutical drugs. A novel biosynthetic pathway to produce legionaminic acid …
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