Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Glycobiology"”.
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Tools for decoding the structure-function relationships of biopolymers in nanotechnology and glycobiology
In this thesis, new tools have been developed for decoding structure-function relationships governing complex biopolymers that have emerged as key players in biology, biotechnology, and medicine. Specifically: (1.) The first part of this thesis addresses the structure-function relationship of …
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The development of hybrid quantum classical computational methods for carbohydrate and hypervalent phosphoric systems
… and AM1* to reproduce key metrics in chemical glycobiology (i.e., sugar ring pucker, phosphate participation in transferase reactions) these methods, in combination with the published parameters, performed very poorly. Using the AM1/d-PhoT and AM1* Hamiltonians I set out to re-parameterize …
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Directed Evolution of Glycan-Binding Proteins
… can benefit from more GBP reagents is bacterial glycobiology. Many pathogens have glycans involved in virulence. One such organism is Campylobacter jejuni. The N-linked protein glycosylation pathway in Campylobacter jejuni is needed for pathogenicity of the organism, as loss of glycosylation …
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Automated synthesis of the Lewis blood group oligosaccharides
… transduction events. Advances in molecular glycobiology rely heavily on straightforward access to structurally defined oligosaccharides, but traditional syntheses of complex carbohydrates have been very laborious. Development of a novel linker and monitoring of each glycosylation reaction …
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Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia Coli
… treatments as well as for further study in glycobiology.
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Design and synthesis of polymers for Corona Phase Molecular Recognition (CoPhMoRe) of carbohydrates
… of human immunity, cell signaling, and glycobiology. For the past decade, significant effort has been made in this field to create new technologies to profile glycans and carbohydrates. Corona Phase Molecular Recognition (CoPhMoRe), the concept introduced from Strano group, generates a …
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To the Frontier of RNA Glycosylation: Novel Insights from Metabolic Labelling
… emphasises the unknown potential of the field of glycobiology and RNA modification. However, the nascent field raises numerous questions concerning the molecule's authenticity, structure, and intricate biological roles, warranting further investigation. This thesis systematically expands upon this …
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Study of Glycan-Mediated Interactions by Photocrosslinking GlcNAc and GalNAc Analogs
… interactions. One of the main challenges in glycobiology is the low affinity of glycan binding events, making them difficult to characterize. To address this challenge, diazirine-functionalized sugar analogs that can be metabolically incorporated into cellular glycans were designed. Upon UV …
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Síntesis y análisis conformacional de glicopéptidos que incorporan ß-glucosa y a-N-acetilgalactosamina
In the last two decades, the glycobiology field, which comprises the study of carbohydrates, glycopeptides and glycoproteins, has attracted considerable attention due to these molecules are involved in many fundamental processes of the life. One of these processes is known as molecular recognition …
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Development and application of tools for glycan characterization
… reiterate the importance of the study of glycobiology by showing how both the development and application of tools to study glycans can provide in new and interesting information about pathological processes related to human health and disease.
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
… In this work, two examples of cyanobacterial glycobiology are considered, each of which involves a cyanobacterium of the Nostoc genus. The first portion of this work details attempts to obtain amino acid sequence information from the enzymes (glycosyltransferases) that are responsible for …
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Studies on fucosylation in Trypanosoma brucei
The biosynthesis of GDP-Fucose, the activated donor for fucose, has been recently shown to be essential in the parasite Trypanosoma brucei. Fucose is a common sugar modification on eukaryotic glycan structures, but it has not been well described in trypanosomatids. To elucidate the role of fucose …
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Studies of the ABO and FORS Histo-Blood Group Systems: Focus on Flow Cytometric and Genetic Analysis
… provided insight into the complex world of glycobiology and established a novel blood group system designated FORS.
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… are important components of the glycobiology toolbox that facilitate manipulation of complex carbohydrate structures. Chapter 3 describes the in vitro selection of deoxyribozymes for O-glycoside cleavage. N-glycosidase deoxyribozymes were reported nearly two decades ago, but …
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The design and development of GPU accelerated algorithms for ab initio integrals and integral derivatives illustrated on ab initio quantum and hybrid QM/MM dynamics
… and configurational structures important in glycobiology. Usually a semiempirical QM method is used however, these have shown variable accuracy for the study of carbohydrate conformation and prevents an analytical investigation of electronic structure. The use of a higher level of theory, …
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Modification and Translocation of the Biofilm Exopolysaccharide Poly-β(1,6)-N-acetyl-D-glucosamine
Bacteria embedded within a biofilm are more resistant to antibiotics and host defenses than those in a planktonic state. Extracellular polysaccharides are a main component of biofilms and have been shown to play an important role in bacterial aggregation, surface attachment, and serve as a …
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Assembly of the O Antigen in the Highly Pathogenic Escherichia Coli Serotype O104:H4
Escherichia coli serotype O104:H4 (ECO104) is an intestinal pathogen that causes severe bloody diarrhea and hemolytic-uremic syndrome, which can lead to kidney failure and death. Lipopolysaccharides (LPS) are important virulence factors in Gram-negative bacteria and our goal is to understand the …
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Development of Mono-de-N-acetylated β-(1→6)-N-acetyl-D-glucosamine Substrates for Characterization of PgaB Hydrolase Activity
Many bacteria form biofilms, surface-attached communities embedded in an extracellular matrix, as a means for survival. The production of the partially de-N-acetylated poly-β-(1→6)-N-acetyl-D-glucosamine (dPNAG) exopolysaccharide is required for biofilm formation in many clinically relevant …
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Sequence-structure-function relationships of glycosyltransferases in families GT43, GT47, and GT64
Extracellular carbohydrates are an essential aspect of biology, playing central roles in cell-cell interaction, cell shape, and infectious disease. In eukaryotes, extracellular glycans and glyco-conjugates are synthesised or matured by glycosyltransferase enzymes in the Golgi apparatus. Most …
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