Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 203 for “"Glycan"”.
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Directed Evolution of Glycan-Binding Proteins
Glycan-binding proteins (GBPs) are commonly used reagents for the study of glycans. They do not require specialized equipment or time-consuming experimental methods, making them widely used tools for basic research and clinical applications. Existing glycan recognition reagents,antibodies and …
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Diabetic foot: microbiology, pathogenesis and glycan studies
… part of the research focussed on utilising the glycan-lectin interactions by developing a simple enzyme linked lectin sorbent assay by employing biotinylated lectins to develop to an enzyme linked lectin sorbent assay (ELLA) on whole cells, Proteinase K treated cells and glycolipids of …
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Computational Studies of Glycan Conformations in Glycoproteins
N-glycans refer to oligosaccharide chains covalently attached to the side chain of asparagine (Asn) residues, and the majority of proteins synthesized in the endoplasmic reticulum (ER) are N-glycosylated. N-glycans can modulate the structural properties of proteins due to their close proximity to …
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Biochemical Characterization of Glycan Assembly Pathway Enzymes
Glycans and glycoconjugates are found in all domains of life and are known to mediate many important and diverse biological processes. Glycan biosynthesis is not templated, and complex glycans are instead assembled en bloc in highly specific and tightly regulated enzymecatalyzed assembly pathways …
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Refining the HIV-1 glycan shield model: dynamics of a heterogeneous envelope trimer and empirical prediction of glycan processing
… Envelope (Env), is covered in asparagine-linked glycans, which interact with the human immune system and are thus important as potential vaccine targets. Laboratory studies have shown that the glycan type and form can differ substantially at each glycan site on Env clones. However, these studies …
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Glycan polymeric prodrugs against pulmonary intracellular alveolar infections
Intracellular pathogens are a major cause of global morbidity and mortality due to their complex and intricate ability to replicate within host cells while evading the innate immune defense system. Many intracellular pathogens, such as F. tularensis and B. pseudomallei, are highly infectious and …
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Interrogation and Manipulation of the IGG FC Glycan
… (IgG) Fc protein backbone and conserved N-linked glycan combine to introduce structural diversity in IgG molecules that in turn, triggers divergent humoral responses. These N-glycans are variably constructed, ultimately leading to families of highly related, but non-equivalent glycoproteins known …
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Complex glycan utilization preferences of human gut bacteria
<p>Complex glycans, making up dietary fiber, have gained significance in recent years as they are the main energy source for the colonic microbiota which are physiologically important for the host health. Understanding glycan utilization strategies of the members of this community is essential to …
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Polyprenyl-dependent glycan assembly pathways in microbial pathogens
Polyisoprenyl-dependent glycan assembly pathways form the basis for the biosynthesis of many complex glycoconjugates. This thesis addresses key aspects of undecaprenyl-phosphate related processes; undecaprenol is the linear polyisoprenol that is utilized by virtually all known bacterial species. In …
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Development and application of tools for glycan characterization
Glycans are essential components of all living things because they function as key elements of cellular membranes and extracellular spaces by mediating cell-cell communication, transduction pathways, and cellular development, function, and survival. Because glycans are secondary gene products that …
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ELECTROKINETIC SEPARATIONS ON MICROFLUIDIC DEVICES FOR N-GLYCAN PROFILING
… and more efficient separation techniques. N-glycans are essential molecules in many living systems and may become important biomarkers for cancer and other diseases in humans. In this work, we developed several microfluidic platforms for separation and analysis of N-glycans. On microfluidic …
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Glycan receptor binding determinants of Influenza A virus hemagglutinin
… which changes its binding specificity towards glycan receptors in the human upper respiratory epithelia (referred to as human receptors). In this thesis, determinants that mediate changes in HA-glycan receptor binding specificity are investigated, with focus on the molecular environments within …
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The Ontogeny of Vaccine-Induced HIV-1 Glycan-Reactive Antibodies
… interface, the CD4 binding site, V1V2-loop plus glycans, and the V3 loop plus glycans. Passive infusion studies with broadly neutralizing antibodies (bnAbs) of various specificities have been shown to be protective as well as control viral load. </p><p>Thus, one component of a protective HIV-1 …
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Label-free carbon nanotube sensors for glycan and protein detection
Nanoengineered glycan sensors may help realize the long-held goal of accurate and rapid glycoprotein profiling without labeling or glycan liberation steps. Current methods of profiling oligosaccharides displayed on protein surfaces, such as liquid chromatography, mass spectrometry, capillary …
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Bioengineering functional polymer systems for glycan-based targeting and drug release
… incorporating different modalities to enable glycan-based targeting to the Pseudomonas a. biofilm and to extend drug release within polymer-drug conjugate manufactured into nanofibers.
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Study of Glycan-Mediated Interactions by Photocrosslinking GlcNAc and GalNAc Analogs
Glycans, along with nucleic acids, proteins and lipids, are essential elements of life. Glycans are involved in multiple biological processes, such as cell signaling, cell adhesion and host pathogen interactions, many of which are achieved through interactions with other molecules, especially …
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Metalloenzymes required for glycan processing and morphological development in Streptomyces lividans
… genes encoding putative enzymes involved in glycan processing. Both these gene clusters are highly conserved in streptomycetes. In Chapter 2 the characterisation of GlxA is reported. It was found to be membrane associated with a mononuclear Cu site and possess a Cys-Tyr redox cofactor capable …
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Structural Characterization of Donkey Lactoferrin: Amino Acid Sequence and Glycan Compositions
… fragmentation, is reported. 26 different glycan compositions were identified, linked to the protein backbone via an amide bond to asparagine residues located at the positions 137, 281 and 476
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Engineering and targeting glycan receptor binding of influenza A virus hemagglutinin
… hemagglutinin (HA) to the sialylated glycan receptors terminated by N-acetyineuraminic acid (Neu5Ac) expressed on the host cell surface. Glycans terminating in Neu5Ac that is a2-6 and a2-3 linked to the penultimate galactose serve as receptors for human- and avian- adapted influenza A …
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Interpretable Supervised Learning and Graph-Based Optimization for Glycan-Lectin Binding
Non-linear biological macromolecules, such as glycans, participate in a wide range of key structural, metabolic, and regulatory functions in all living organisms. Many of these essential roles involve interactions with glycan-binding proteins called lectins. As a result, there is particular …
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