Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Glutathionylation"”.
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Palmitoylation and Glutathionylation of Key Sarcolemmal Signalling Proteins in Cardiac Muscle
… addition of the tripeptide glutathione, known as glutathionylation. Palmitoylation can regulate many processes such as intracellular membrane targeting and membrane interactions. Glutathione acts as a redox buffer and antioxidant to prevent proteins from irreversible oxidative damage. Protein …
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The Role of Protein S-glutathionylation on Ca2+ Signaling in Cultured Aortic Endothelial Cells
… ([Ca2+]i). An increase in protein S-glutathionylation is a common response to oxidative stress; however, the role of glutathionylation in Ca2+ homeostasis and Ca2+ signaling processes is not well understood. Diamide, a membrane-permeable oxidizing reagent, rapidly and reversibly …
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The Effects of S-glutathionylation on the Structure and Function of the Mitochondrial Calcium Uniporter (MCU)
… MCU is a regulatory component of the channel. S-Glutathionylation of Cys97 on the MCU-NTD leads to robust MCU activation and increased matrix Ca2+. Here, I characterized the biophysical and structural changes induced by Cys97 S-glutathionylation by applying optical spectroscopy, light scattering, …
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Diverse functions of LanC-like proteins in mammalian cells
… acids containing peptides. The same C-glutathionylation was also observed by using dehydroamino acid containing proteins as substrates. These data show that LanCL proteins have flexible capabilities to catalyze C-glutathionylation on different substrates. GC-MS analysis showed that the …
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Metal complexes of non-heme ligands: biological applications
… 20S proteasome) by non–heme iron complexes, and glutathionylation of non–heme cobalt complexes mimicking the N5 coordination environment like that of biologically important cofactor cobalamin or B<sub>12</sub> (Cbl) are reported. Different non–heme ligand sets or inhibitors were used to …
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Investigation and application of substrate tolerant thioether forming enzymes
… function in a similar way as LanC, by catalyzing glutathionylation of dehydro amino acids to afford the small molecule lanthionine in mammalian systems. To test this hypothesis, in chapter 3, I developed two LC/MS/MS methods to quantify endogenous lanthionine concentrations in both wild type and …
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Insights into the role of PFKFB3 in cancer
… the activation of PFKFB3, induced by S-glutathionylation, decreases cellular Fru-2,6-P2 levels and reroutes the glucose metabolic flux from glycolysis to the PPP, maintaining oxidative homeostasis in cancer cells. The ability of PFKFB3 to control ROS levels can not only support cancer …
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Novel strategies to increase Sirtuin-1 activity in aortic aneurysm
… RESULTS: OPTM of SirT1 cysteines, specifically S-glutathionylation, were increased while SirT1 activity was decreased in aortas and VSM cells from WT and Fbn1mgR/mgR mice. SirT1 activity impairment was associated with increased MMP2/9 activity. Importantly, overexpression of an oxidation-resistant …
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Computational investigation of early events in plant growth signaling
… finding that BAK1 is deactivated in vitro by S-glutathionylation. Simulating BAK1 with each of its three solvent-accessible cysteine residues individually S-glutathionylated, we find that modification of C408 induces further αC helix unfolding due to direct interactions, while causing global …
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Catalytic and non-catalytic mechanisms involved in Glutathione S-transferase Pi mediated cytoprotection
… levels of glutathione disulphide and protein S-glutathionylation, a reversible post-translational modification involved in thiol protection. Subsequent analysis of GSTP1 silencing in this cell line led to the identification of potential novel pathways which may be mediated by GSTP1.This study …
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Multidrug resistance proteins (MRP) transport the cytokine/nuclear protein High Mobility Group Box 1 (HMGB1) across membranes
… Here I demonstrate the necessity of cysteine glutathionylation for the translocation inside secretory Iysosomes, and the direct involvement of Multidrug Resistance Proteins (MRP/ABCC) in the transport process. I started with the observation that HMGB1 and IL-1 beta, another leaderless …
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D-METHIONINE (D-MET) MECHANISMS UNDERLYING OTOPROTECTION FROM NOISE- AND AMINOGLYCOSIDE-INDUCED HEARING LOSS
… may be a result of significantly increased s-glutathionylation activity in the cochlea. Specific aim 5 tested D-met protection from impulse noise exposures. D-met dose response, rescue, and antioxidant enzyme assay protocols, similar to those in specific aims 1, 3, and 4 in steady state …