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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"Glucuronidase"”.
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Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction
… of an acidic model protein, recombinant β-glucuronidase (rGUS), from transgenic tobacco with high yield and purity. Aqueous two-phase extraction (ATPE) is a powerful technique for separation and purification of proteins, and has the potential to replace an expensive chromatography step for …
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The Determination of Glucuronides in Biological Fluids by Lucigenin Chemiluminescence (beta-Glucuronidase, Glucuronic Acid, Immobilized Enzyme, (Hplc))
The use of certain chemiluminescence reactions as detection techniques for organic compounds has been explored during the past decade. In this research project the chemiluminescence reaction between alkaline lucigenin (10,10'-dimethyl-9,9'-biacridinium nitrate) and glucuronic acid (an important …
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Lysosomal Enzymes in Undifferentiated and Differentiated HL-60 Cells
… Elastase, chymotrypsin, ribonuclease, β-glucuronidase and lysozme concentrations were determined in the lysosomal fraction and whole cell lysates. The amount of chymotrypsin and elastase increased significantly in cells which had differentiated to macrophage-like cells. The intrracellular …
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Comparison of Extraction Techniques for Δ-9-Tetrahydrocannabinol from Urine
… research, either a strong alkaline solution or β-glucuronidase has been used to cleave the bond between the Δ-9-THC and glucuronic acid before an alkaline extraction. Δ-9-THC has a pKa of 10.6 and therefore is always extracted at an alkaline pH. In this study, both a strong solution ofNaOH and the …
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Expression and activity of enzymes in the human placenta: pharmacological & toxicological consequences in AZT therapy
… perfusion model into the first trimester. UGT, β-glucuronidase, Cytochrome p4501A (CYPIA) and CYP reductase were present and active in the human placenta. CYP2E1 protein was expressed but not active. CYPIA, CYP2EI and the UGT2B subfamily were expressed across gestation, but the UGTIA subfamily was …
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Adding Upstream Sequence and a Downstream Reporter to the Bile Acid Inducible Promoter of <I>CLOSTRIDIUM scindens</I> VPI 12708
… a bile acid inducible promoter, and a ϐ- glucuronidase reporter. Cloning strategies utilized PCR to amplify desired DNA fragments and sewing methodology to combine DNA fragments. DNA fragments were ligated into plasmids and transformed into competent <i>E. coli</i>. Transformants were …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
… use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal D-glucuronic acid residues from oligosaccharides present in the reaction mixture. This action prevented the oligosaccharides from participating in the transglycosylation reaction. Conditions were also …
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Conformational Analysis of Disordered Proteins Using H/D Exchange Mass Spectrometry
… H/D-MS study of the conformational dynamics of β-glucuronidase was also conducted. The destabilization of β-glucuronidase that results from the W492G mutation and the partial conformational rescue that results from indole addition were analyzed. A new method to interpret and normalize H/D-MS data …
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The Development of Certain Enzymes and Iso-Enzymes During Normal Pregnancy
… n-acetyl-β-galactosaminidase and β-glucuronidase, have been found to progressively increase. Dipeptidase and tripeptidase were not found to increase during pregnancy. A wide scatter of results was found during each week of pregnancy which was due in part to poor reproducability of …
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Diagnostic use of hair analysis for the detection of misuse of amfetamines and cannabinoids
… and pre-treatment methods, alkaline (1M NaOH), β-Glucuronidase (helix pomatia), methanol (MeOH) and acid (0.1M HCI) were used on these and compared. The best recovery for amphetamine was obtained using the β-glucuronidase pre-treatment method and this extract was also found to be cleaner than the …
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Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
… phosphatase, acid phosphatase, lysozyme, $\beta$-glucuronidase, and myeloperoxidase was quantitated and was not affected by treatment.
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Tumor-Specific STING Agonist Synthesis via a Two-Component Prodrug System
… the active compounds in tumors overexpressing 𝛽-glucuronidase. These results exemplify the use of small-molecule recognition for on-site generation of active compounds from benign precursors.
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I. Etablierung eines induzierbaren Suizidsystems zur Identifizierung von Mutanten der salizylsäureabhängigen Signaltransduktion II. Expression von tierischen Signaltransduktionskomponenten in Tabak zur Herstellung eines induzierbaren Expressionssystems
… unterscheiden zu können, wurde die β-Glucuronidase unter der Kontrolle des gleichen Promotors als zweites Reportergen eingebracht.Das regulatorische Protein NPR1 ist eine entscheidende Komponente bei der durch Salizylsäure induzierten Aktivierung der späten" Abwehrgene. Durch Kreuzung …
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Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… assay of the reporter uuid gene encoding B-glucuronidase (GUS). Increases in plasmid DNA resulted in increases in GUS activity. Maximal GUS activity was observed at field strength of 950 V/cm, protoplast density of 2 x 10⁶/ml, and KCl concentration of 125 mM in the electroporation buffer. …
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Analysis of organ-specific gene expression in soybean
… of a foreign gene. The reporter gene $\beta$-glucuronidase (GUS), was linked to the 5$\sp\prime$ and 3$\sp\prime$ sequences of the lectin gene and transferred into Arabidopsis thaliana and Nicotiana tabacum. GUS activity assayed on transformed tissues revealed seed-specific expression of GUS …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the different field strength conditions tested, maximum GUS activity was …
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Toxicology and herb-drug interaction of selected anti-hypertension plants used by lay persons in Northern KwaZulu-Natal (South Africa)
… were the CYP3A4 inhibition assay, the beta-glucuronidase inhibition assay as well as the carboxylesterase inhibition assay. These assays were chosen according to the enzymes involved in the metabolism of the hypertension conventional drugs. The overall toxicity profile of the plant extracts …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… droplet sorting (FADS) for β-glucuronidase activity leading to the discovery of hit SN243. This enzyme belongs to the glycoside hydrolase (GH) family 3, a family that had no previous record of β-glucuronidase activity at the outset of this study. Detailed functional and …
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One- and Two -Photon States for Quantum Information
… expression level based on the activity of beta-glucuronidase of 158 +/- 9 units/mg protein (mean +/- SEM; n=3; variance within SC lines are expressed as standard error of the mean SEM) indicating a significantly higher level of expression, as compared to MC lines that had a much lower expression …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
… Agrobacterium Ti plasmid. The reporter gene, b-glucuronidase (GUS) was inserted proximal to AAVS1 in the plasmid for use in screening for the presence of T-DNA. In addition, it will serve as an indicator of the expression level expected for transgene inserted into AAVS1 by recombinant AAV. PCR …
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