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Showing 1 to 20 of 23 for “"Glucosidases"”.
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Isolation and characterization of rice (Oryza sativa L.) β-Glucosidases
… made up of 60 kD monomers. In rice dry seeds, β-glucosidases were distributed in the aleurone layers and embryo parts. β-glucosidase inhibitors suppressed germination at the activation stage. The inhibitors Suppressed the expression of α-amylase and β-glucosidase during germination detected at …
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Dietary carbohydrates influence the structure and function of the intestinal alpha-glucosidases
… maltose) are the main substrates for the α-glucosidases at the intestinal brush border. Here, maltose was shown to induce the formation of a higher molecular weight (HMW) sucrase-isomaltase (SI) species in Caco-2 cells that sorts more quickly to the enterocyte surface to act as a digestive …
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Mechanism of Substrate Specificity and Catalysis in Retaining β-Glucosidases From Maize and Sorghum
β-glucosidases catalyze the hydrolysis of aryl and alkyl β-D-glucosides as well as glucosides with a carbohydrate moiety. The maize β-glucosidase isozymes Glu1and Glu2 hydrolyze a broad spectrum of substrates in addition to its natural substrate DIMBOAGlc, while the sorghum β-glucosidase Dhr1 …
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Purification and characterisation of novel recombinant β-glucosidases from aspergillus with application in biofuel production
β-glucosidases are important components of the cellulase enzyme system in which they not only hydrolyse cellobiose to glucose, but also remove the feedback inhibition effects of cellobiose on exoglucanase and endoglucanase thereby increasing the rate of cellulose degradation to fermentable sugars. …
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Characterisation of recombinant beta-glucosidases from Thermomyces lanuginosus SSBP and investigation of their synergistic potential in cellulose hydrolysis
Submitted in complete fulfilment for the Degree of Master of Applied Science in Biotechnology in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa, 2020.
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Determination of the Binding Site and the Key Amino Acids on Maize β-Glucosidase Isozyme Glu1 Involved in Binding to β-Glucosidase Aggregating Factor (BGAF)
… However, sequence comparison between sorghum β-glucosidases (dhurrinases, Dhr1 and Dhr2), to which BGAF does not bind, and maize β-glucosidases, and an examination of the 3-D structure of Glu1 suggested that the BGAF binding site on Glu1 is much smaller than predicted previously. To define more …
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Isolation And Characterization Of Natural Alpha-Glucosidase Inhibitors From Antioxidant Rich Red Wine Grapes (vitis Vinifera)
… to exhibit an inhibitory activity against alpha- glucosidases. Alpha-glucosidases, in turn, play a major role in controlling starch digestion and therefore postprandial blood glucose, a target for diabetes management. </p> <p> <strong>Objective:</strong> This study aims to investigate the …
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Purification and characterization of fungal β-glucosidase enzymes
… and one of the key enzyme group is β-glucosidases. These enzymatic systems are mainly adopted from wood-decaying fungi. The overall enzymatic system consists of different types of cellulases that first degrade the crystalline cellulose to oligosaccharides and cellobiose. In the final …
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INHIBITION OF ACTIVITIES OF INDIVIDUAL SUBUNITS OF INTESTINAL MALTASE-GLUCOAMYLASE AND SUCRASE-ISOMALTASE BY DIETARY PHENOLIC COMPOUNDS FOR MODULATING GLUCOSE RELEASE AND GENE RESPONSE
… particularly the intestinal mucosal alpha-glucosidases [Maltase-Glucoamylase (MGAM) and Sucrase-Isomaltase (SI)], has a potentially important role in both the etiology and treatment of this metabolic disorder. Inhibition of the activities of the intestinal alpha-glucosidases may be a …
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Dissecting the jasmonate singalling pathway in Arabidopsis thaliana
… genes in Arabidopsis thaliana. β-glucosidases catalyse the breakdown of glucosinolates into glucose and sulphate releasing toxic by-products which play a role in plant defence against insects and pathogens.
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Molecular Design and Mechanistic Characterization of Glycoside Hydrolases using Computational and Experimental Techniques
… including endoglucanases, cellobiohydrolases and glucosidases that work synergistically to solubilize crystalline cellulose efficiently. The dependence of hydrolysis reaction rate on temperature predicts that large increases in performance and decreased enzyme cost would be achieved if the …
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Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution
… myoglobin and glycosyl hydrolase family 1 beta-glucosidases will be examined with molecular modeling methods. Results of our analyses provide information about DNA flexibility and packaging, internal migration of ligands in a small protein, and substrate specificity of an enzyme system.
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Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
… with respect to the occurrence of two distinct β-glucosidases being present in sorghum.
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Diastereoselective acylation of trans-2-substituted-cyclohexanols and glycosidase inhibition studies
… values in excess of the activity of β-D-glucosidases. More interestingly, the same compounds showed a marked enhancement of α-D-galactosidase activity by as much as 35%.</p>
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Maize β-glucosidase substrate specificity and natural substrates
Plant β-glucosidases (β-D-glucoside glucohydrolases, E.C. 3.2.1.21) are known to function in defense in cyanogenic systems, and may also function in the metabolism of phytohormone glucosides and glucosides of other secondary plant products. Maize (Zea mays L.) β-glucosidase is a homodimer of 60 KD …
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Grape Extracts for Type 2 Diabetes Treatment Through Specific Inhibition of α-Glucosidase and Antioxidant Protection
… and specific inhibition of intestinal α-glucosidases.
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African starchy foods, gastric emptying, and starch digestion in Malian stunted children
… the healthy group, indicating that the &agr;-glucosidases compensate for the &agr;-amylase insufficiency, and particularly well in the stunted group. A thicker porridge and its &agr;-amylase thinned counterpart were also digested well by the stunted group. ^ Overall, this work shows value of …
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Bioprospecting the Regional Diversity of Australian Wine Microbiota
… applications in wine clarification, and seven β-glucosidases, with potential applications in wine aroma and flavour, were chosen for heterologous expression in Pichia pastoris and Saccharomyces cerevisiae. Two of the selected polygalacturonases exhibited significant levels of activity at wine pH …
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Cellulolytic enzyme systems of Myrothecium verrucaria
… It was found to hydrolyze several β-aryl-glucosidases with varying ease. In the presence of various alcohols it transferred the glucose moiety of the glucoside to the alcohol. With methanol the product was methyl glucoside, but it was not possible to establish the configuration of the …
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