Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Glucaric acid"”.
-
Improvement of D-glucaric acid production in Escherichia coli
D-glucaric acid is a naturally occurring compound which has been explored for a plethora of potential uses, including biopolymer production, cancer and diabetes treatment, cholesterol reduction, and as a replacement for polyphosphates in detergents. This molecule was identified in 2004 as a "Top …
-
Pathway and protein engineering for improved glucaric acid production in Escherichia coli
… for the renewable production of chemicals. Glucaric acid was identified as a "top value added chemical from biomass" by the Department of Energy in 2004, and a biological route for its production from glucose in E. coli was developed in our lab in 2009. Two of the pathway enzymes, …
-
Retrobiosynthesis of D-glucaric acid in a metabolically engineered strain of Escherichia coli
… a synthetic pathway for the production of D-glucaric acid has been designed and constructed in E. coli. To this end, three disparate enzymes were recruited from three different organisms, and the system perturbed by this introduction of heterologous genes was analyzed. Limiting flux through …
-
Dynamic control of Escherichia coli central metabolism for improvement of glucaric acid production
… of growth versus production is the pathway for D-glucaric acid production from glucose developed in the Prather Lab (Moon et al., 2009). Previous work with the glucaric acid pathway has indicated that competition with endogenous metabolism for glucose-6-phosphate (G6P) may limit carbon flux into …
-
D-glucaric acid excretion: its positive association with gender, tobacco, caffeine, marijuana, and vegetarianism in humans
The urinary excretion of D-glucaric acid (DGA) has been used as a nonspecific measure of the induction of hepatic enzymes associated with drug metabolism in man. A survey of 124 nonmedicated men (18-56 years of age), who kept a 5-day food and beverage intake record and collected their total urinary …
-
Characterizing uronate dehydrogenase from P. syringae as a step towards expressing a retro-biosynthetically derived glucaric acid pathway in E. coli
… this laboratory for the microbial production of glucaric acid. In addition to design, efforts have been made in order to express these pathways inside E. col. For the production of glucaric acid in E. coil, toxicity of this organic acid as well as the uptake efficiency of glucose, the starting …
-
Biosensor-based strategies for improving pathway production in Escherichia coli
… alternative to petroleum-based processes. D-glucaric acid, a Department of Energy top value-added chemical from biomass, is a precursor to polymers such as nylons and used in detergents. An engineered metabolic pathway requiring three heterologous enzymes to convert glucose into glucaric acid …
-
Synthesis of Higher Molecular Weight Poly(D-glucaramides) and Poly(aldaramides) as Novel Gel Forming Agents
… form ion exchange resin to give D-glucaric acid, which was then reacted with a diamine. The glucarate portion of the salt was activated for polymerization through esterification in HCl/methanol. Polymerization was initiated by basification of the resulting mixture. The molecular …
-
Synthesis of Higher Molecular Weight Poly(D-glucaramides) and Poly(aldaramides) as Novel Gel Forming Agents
… form ion exchange resin to give D-glucaric acid, which was then reacted with a diamine. The glucarate portion of the salt was activated for polymerization through esterification in HCl/methanol. Polymerization was initiated by basification of the resulting mixture. The molecular …
-
Dynamic regulation of bacterial metabolic pathways using autonomous, pathway-independent control strategies
… boost in myo-inositol (MI) and increasing glucaric acid titers from unmeasurable quantities up to >0.8 g/L. With a focus on industrial application, consistency of device performance was verified in benchtop bioreactors, achieving nearly 10-fold and 5-fold boosts in specific titers of …