Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Global DNA methylation"”.
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Defining CP-CML patient subsets associated with poor imatinib uptake and response.
… new biomarkers for CP-CML; and 3) determine the global DNA methylation profile of CP-CML, with particular focus on very low OA, and ascertain whether aberrant epigenetic programming may underlie poor imatinib response. Specific lineage differences were identified, with patients defined as very …
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Cardiovascular effects of lead and mercury and their mixtures in rats
… stress, kidney function and damage, and global DNA methylation as potential mechanisms of action for the development of elevated blood pressure. Results for lead showed an increase in oxidative stress but not mercury. While only lead was associated with kidney damage, only inorganic …
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The role of epigenetic changes in chemoresistant breast cancer cells
… wide screens of microRNA and gene expression, DNA methylation and complete genome hybridization. We identified three novel microRNA interactions involved in the chemoresistant phenotype. These three microRNAs displayed depressed expression in the resistant cell lines and we were able to …
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The impact of chronic loss of STAG2 on DNA damage repair and the epigenome in acute myeloid leukaemia
… the 3D genome, controlling gene expression and DNA repair. The consequences of a STAG2 mutation on Cohesin function is not well understood and in particular, the effects on DNA damage repair remain to be fully elucidated. In addition, it is likely that the epigenome is affected by Cohesin’s …
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Effect of rumen-protected methionine on placenta metabolism, animal performance, and immune-metabolism of dairy cows during the periparturient and early lactation periods
… pathway, and MTR activity. No differences in global DNA methylation or mRNA expression of the DNA methyltransferases were observed between Male CTR and Male MET. Male MET cows delivered heavier calves than Male CTR; however, this difference was not maintained until 9 weeks of age. Female MET …
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Maternal rumen-protected methionine supplementation alters dairy calf development, postnatal growth, plasma biomarkers, and liver one-carbon metabolism
… used for RNA sequencing, protein expression, and global DNA methylation. Blood for biomarker analyses and innate immune function assays was harvested at birth (before colostrum feeding), 7, 21, 42 and 50 d of age. Whole blood was challenged with enteropathogenic bacteria (E. coli 0118:H8), and …
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Diet, genetic and epigenetic signatures in women of childbearing age from a Mediterranean population: perspectives for public health
… Mediterranean diet (MD), folate intake and global DNA methylation in women of childbearing age. A total of 177 women were enrolled in a cross-sectional study. Folate intake and MD were assessed by a validated food frequency questionnaire. LINE-1 methylation level in leukocytes was measured …
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Expression and regulation of genes associated with CNS regeneration and plasticity
… of dissociated adult DRG neurons 14 4. Genomic DNA extraction for MeDIP-seq 14 5. Methylated DNA immunoprecipitation and sequencing 15 6. Bioinformatics analysis 15 7. Bisulfite sequencing 17 8. Chromatin immunoprecipitation assay 18 9. ChIP-PCR primers 19 10. qRT-PCR primers 20 11. Methylation …
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ZSCAN4 AND REPLICATION STRESS: SAFEGUARDING GENOME STABILITY IN MOUSE EMBRYONIC STEM CELLS
The accuracy of DNA replication is essential for maintaining genome stability. However, replication can be disrupted by both endogenous and exogenous factors, leading to a condition known as replication stress (RS). RS slows down and eventually stalls replication forks. If stalled forks are not …
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Epigenetic effects of benzo(a)pyrene in Fundulus heteroclitus and Danio rerio
DNA methylation is one of the epigenetic mechanisms that controls gene expression and is vulnerable to early life environmental toxicant exposures. Our goal was to use two fish models, Fundulus heteroclitus and Danio rerio (zebrafish), to study the benzo(a)pyrene (BaP) effects on DNA methylation …
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Perinatal Hypoxia Exposure on the Developing Heart: The Role of Endothelin-1
… this effect was associated with an increase in global DNA methylation. Our next section of the study simulated the major clinical problem of premature birth and the anoxic episodes that often accompany it. We demonstrated that early neonatal anoxic episodes decrease cardiomyocyte proliferation …
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Molecular and cellular mechanisms of energy homeostasis in birds
… in LWS. Stress exposure resulted in increased global DNA methylation and DNA methyltransferase activity in the arcuate nucleus of the hypothalamus in LWS. Moreover, there was decreased binding to methyl-CpG-binding domain protein 2 in the promoter of corticotropin-releasing factor (CRF) because …
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DNA Methylation and DNA Methyltransferases in the Clonal Raider Ant, Ooceraea biroi
<p>DNA methylation and DNA methyltransferase enzymes (DNMTs) are ubiquitous and predate the origin of eukaryotes. In animals, DNA methylation primarily is carried out by DNMT1, which targets hemi – methylated DNA and maintains methylation patterns through cycles of cell replication, and DNMT3, the …
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Physiological and Biochemical Aspects of Methionine Isomers and Precursors in Broilers
… aspects, including growth performance, global DNA methylation and methyltransferase activity, blood antioxidant profile (e.g., acute phase protein, leukocyte count), intestinal nutrient transporter gene expression, Met converting enzyme gene expression and activity, oxidative stress …
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Bioinformatic and biological analysis of DNA methylation in the human genome
Epigenetics is the study of DNA related information heritable through meiosis and mitosis that does not include the DNA code itself. DNA methylation is currently the most studied epigenetic mark. It is a part of the inactivation of the X chromosome, defense against transposable elements and the …
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Investigating DNA Methylation Changes in a Preclinical Murine Model of Cutaneous Squamous Cell Carcinoma
… study, we provide a comprehensive analysis of global DNA methylation of this murine model and compare it to the methylome of human cSCC. We found that methylation changes in the murine cSCC tumors occur predominantly at regions of potential regulatory function, such as enhancers, promoters and …
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Characterising the role of TET2 in oestrogen receptor positive breast cancer
… are thought to be capable of binding methylated DNA, whilst TET2 is also implicated in DNA methylation pathways through its iterative conversion of methylated DNA to several additional DNA modifications, 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine. These results implied a …