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Showing 1 to 20 of 87 for “"GlcNAc"”.
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Understanding tetratricopeptide repeats of <i>O</i>-GlcNAc transferase
… with O-linked N-acetyl-β-D-glucosamine (O-GlcNAc) was serendipitously discovered in 1984. During the following decades it became clear that intracellular O-GlcNAc levels respond to various stimuli, suggesting that this modification is dynamic and may represent a novel cellular signalling …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… proteins (> 1000) with -N-acetylglucosamine (O-GlcNAc / O-GlcNAcylation) is a highly dynamic and essential process in metazoa. The attachment and removal of the sugar residue is regulated by two antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto protein substrates …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
<p>O-linked β-N-acetylglucosamine (O-GlcNAc) exerts myriad effects on protein localization, activation, inhibition, stability, conformational changes, or degradation. However, the biochemical effects of O-GlcNAc on the vast majority of substrates is unknown. Recently, we and others have shown that …
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O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… by O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential signaling mechanism that affects diverse processes such as cell cycle, metabolism, and death. Aberrant signaling has been implicated in numerous human diseases including cancer and neurodegeneration. However, major aspects of …
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Genetic encoding of a stable <i>O</i>-GlcNAc analogue
… of intracellular proteins is known as <i>O</i>-GlcNAcylation, an essential posttranslational event in mammals and most other metazoa, catalysed by a unique <i>O</i>-GlcNAc transferase (OGT) and removed by an <i>O</i>-GlcNAc hydrolase (OGA). Despite the existence of a single writer/eraser pair, …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological functions with …
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Study of Glycan-Mediated Interactions by Photocrosslinking GlcNAc and GalNAc Analogs
… on diazirine-functionalized N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) analogs, GlcNDAz and GalNDAz. I will demonstrate the incorporation of GlcNDAz into N-linked glycans using complementary methods and the applications of GlcNDAz in identification of binding partners of two …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification that is regulated by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA). While it is elusive how O-GlcNAc alters protein function, altered O-GlcNAc levels are associated with human diseases. …
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Computational structure analysis and prediction of Ser/Thr modified by O-GlcNAc in human proteins
… modifications of proteins by O-GlcNAcylation with a computational biology approach. The O-GlcNAc transferase (OGT), the enzyme that catalyses the protein O-GlcNAcylation, targets specific Serines and Threonines (S/T) of intracellular proteins. However, while other …
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Structural and Functional Characterization of B. Anthracis Udp-Glcnac 2-Epimerase and Salmonella Secreted Effector I
… of the Bacillus anthracis cell-wall enzyme UDP-GlcNAc 2-Epimerase and the Salmonella secreted virulence effector SseI. UDP-GlcNAc 2-Epimerase is an essential B. anthracis enzyme required for formation of the peptidoglycan cell wall precursor UDP-ManAc. We have solved the epimerase's crystal …
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Solution and solid phase synthesis of N,N'-diacetyl chitotetraoses
… The β-(1→4) linked N-acetylglucosamine (GlcNAc) moiety is a frequently occurring structural unit in various naturally and biologically important oligosaccharides and related conjugates. Chitin which is the most abundant polymer of GlcNAc is widely distributed in nature whereas the related …
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The Effects of a 5-Day High-Fat Diet on Skeletal Muscle O-GlcNAcylation
… O-linked B-D-N-acetylglucosamine, known as O-GlcNAc, has recently been identified as an important posttranslational modification that responds to nutrient sensing and cellular stress. Unlike other PTMs, O-GlcNAc has only two cycling enzymes. Because of its novelty, little research has been …
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Structural analysis of cytokine signaling modulators
… in cells with an oncogenic background. GlcNAc feeding reestablishes lattice formation, cytokine signaling and tumorigenesis.</p><p>A MALDI-TOF analysis was employed to identify the N-glycans involved in modulating signal transduction in wild type and null Mgat 5 cells grown with …
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Reductionist animal models to probe protein O-GlcNAcylation
Protein O-GlcNAcylation is a reversible type of glycosylation of serine and threonine residues of nucleocytoplasmic proteins occurring in all animals examined to date. Its installation on protein substrates is carried out by O-GlcNAc transferase (OGT), and its removal by O-GlcNAc hydrolase …
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Development of a novel liquid chromatography based tool to study post-translational modifications
… chosen to be the basis of this study was the O-GlcNAc modification. Using the C2C12 mouse myoblast cell line, it was determined that the optimal conditions for producing lysates containing increased yields of O-GlcNAc modified proteins was to treat differentiated C2C12 cells with 10nM insulin, …
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Oligosaccharid-gerichtete RNA-Aptamere
… Ziele wurden vier Oligosaccharide Gal-1,4-b-GlcNAc-1,2-Man-OR; a-Neu5Ac-2,3-b-Gal-1,4-b-GlcNAc-1,2-Man-OR; 2,4-(b-Gal-1,4-b-GlcNAc)2-a-Man-OR und 2,4-(a-Neu5Ac-2,3-b-Gal-1,4-b-GlcNAc)2-a-Man-OR mittels chemisch-enzymatischer Verfahren mit zwei unterschiedlichen Spacern synthetisiert. Die …
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Beta-o-glicosilación de péptidos que incorporan aminoácidos no naturales
… Finally, despite the importance of beta-O-GlcNAc glycosylation in fundamental biological processes, sparse structural information is known about beta-O-GlcNAc-Ser/Thr motifs. For this reason, we report the synthesis and conformational analysis of the simplest model glycopeptides derived …
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