Global ETD Search
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Showing 1 to 17 of 17 for “"Giant Unilamellar Vesicles"”.
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Characterization of Membrane Organization and Dynamics in Giant Unilamellar Vesicles Assembled From Rat Kidney Membrane Extracts and Fragments
Fluctuation correlation spectroscopy (FCS) measurements performed on the intact BBM reveal that the protein-lipid dynamics in renal BBM is complex with a large range of diffusing species. In particular, the dynamics of NaPi-IIa was related to the perturbations in the BBM as a result of dietary …
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Spatial correlation of lipid phase fluctuations in model membranes studied with two-photon fluorescence microscopy
… of different composition were reconstituted in Giant Unilamellar Vesicles (GUVs) and labeled with the environment sensitive dye LAURDAN whose spectral properties are sensitive to the lipid phase. The Generalized Polarization (GP) function was used to quantify the lipid phase state as detected by …
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Interactions of functionalized vesicles in the presence of Europium (III) Chloride
… functional units into large (LUV's) and giant unilamellar vesicles (GUV's). Electrolyte solutions containing di- and trivalent ions were used to induce inter-membrane interactions. Measurements performed with isothermal titration calorimetry (ITC) revealed that interaction between EuCl3 …
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A Bottom-Up Approach to Building an Artificial Cell by Self-Assembly
… of biomolecules. The formation of lipid based vesicles is an inherent element of artificial cell development, which requires reliable techniques to prepare vesicles of cell size. The most widely applied methods have been evaluated here based on the size, quality and abundance of vesicles formed …
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Protein Dynamics: Studies of Adenylate Kinase Mutants From Escherichia Coli and Characterization of Adenylate Kinase Isoforms From Murine Cells. Applications of Fluorescence Spectroscopy and Microscopy
… its interaction with a model membrane system (giant unilamellar vesicles (GUVs)). AK1beta showed no binding affinity to GUVs made from POPC in the fluorescence imaging study. However, when AK1beta was co-expressed with N-myristoyl-transferase (NMT), an enzyme that can myristoylate proteins with …
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
… probe in artificial lipid bilayer membranes of giant unilamellar vesicles was investigated. In another series of experiments, the photoconversion and photobleaching behavior of the fluorescent protein Kaede-GFP was characterized and protein subpopulations were identified.
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Toca-1 driven actin polymerisation at membranes
… interaction and the interaction of Toca-1 with giant unilamellar vesicles. FBP17, a homologue of Toca-1, can oligomerise to form 2D flat lattices and 3D tubules on membranes. Proteins of the Toca-1 family have previously been implicated in actin polymerisation in cell-free systems and during …
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Light-induced transformations in biomembranes
… changes in model membranes, namely, giant unilamellar vesicles (GUVs). The mechanism of the changes in the GUVs’ morphology caused by isomerization of the photosensitive molecules has been previously explored but still remains elusive. We examine the morphological reshaping of GUVs in …
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Lipid and Protein Sorting by, and Generation of, Membrane Curvature in Model Systems
… phase separation. Furthermore, phase-separated giant unilamellar vesicles from which are pulled thin cylindrical tubes are employed to understand domain nucleation in curvature gradients, complemented by measurements of the biophysical force required to pull such tubes. These results indicate …
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A Lab-on-a-chip System for the Standardised Characterisation of Membrane Active Antimicrobials
… an element for the high-throughput generation of Giant Unilamellar Vesicles (GUVs) in physiological salt concentrations on demand. Thousands of GUVs are individually immobilised downstream in hydrodynamic traps connected to separate perfusion inlets that facilitate the total fluid exchange of the …
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Understanding the membrane biophysics of alpha-Synuclein and its role in membrane curvature induction and structural remodeling
… whereas in vitro studies of small lipid vesicles and aSyn demonstrate an inhibition of vesicle fusion. In addition, numerous biophysical studies have identified potential curvature sensing and curvature inducing characteristics for aSyn, however the mechanism behind these processes is not …
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Model Systems For Studies At The Plasma Membrane: Investigating Lipid Phase Behavior And Protein-Mediated Membrane Remodeling
… Using fluorescence microscopy imaging of giant unilamellar vesicles (GUVs), we found that this nano-to-macro transition exhibits a regime of patterned fluid domains within the liquid coexistence region of this four-component system. Temperature-dependent FRET and microscopy studies …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… between Myo1c and kinesin-1 on deformable giant unilamellar vesicles (GUVs) at physiologically relevant micropatterned arrays of sparse microtubules crossing dense actin filaments. We found that the lipid composition of GUVs regulates its frequency of tubulation along microtubules by …
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Adaptive Spline-based Finite Element Method with Application to Phase-field Models of Biomembranes
… the evolution of microdomains on the surface of Giant Unilamellar Vesicles. The chemical energy is described by a Cahn-Hilliard type density functional that characterizes the line energy between domains of different species. The generalized Canham-Helfrich-Evans model provides a description of …
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Experimental and Computational Modelling of Tissue Rheology and Mechanics in Morphogenesis
… tissue model is presented, which consists of giant unilamellar vesicles decorated with cell-cell linker molecules. Cell-cell adhesion and cell membrane tension are controlled to influence tissue-scale mechanical properties. The first results chapter of this part introduces novel protocols on …
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Microfluidic Transport Studies on Lipid Vesicles
… Assembly (OLA) technique to obtain lipid vesicles which serve as model membrane for our studies. We perform a literature review, where we discuss OLA and other techniques to obtain liposomes in detail, before we provide a biophysical analysis of liposomes generated with OLA and compare …
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Quantification of membrane transport rates using optofluidics
… Assembly (OLA) - an existing microfluidic Giant Unilamellar lipid Vesicle (GUV) formation method – is refined by the integration with a novel Pinched Flow Fractionation (PFF) purification module. PFF removes formationassociated residues at a range of length-scales, from surfactant molecules …