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Showing 1 to 20 of 21 for “"Germline Stem Cells"”.
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MicroRNA Expression Profiling of Multipotent Adult Germline Stem Cells
… pluripotent werden und als "multipotent adult germline stem cells" (maGSCs) bezeichnet werden. Vor einiger Zeit wurde eine Gruppe nicht codierender RNAs identifiziert, die sogenannte microRNAs (miRNAs), die eine Schlüsselrolle in der embryonalen Entwicklung und Pluripotenz von Embryonalen …
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Platiscity of C. Elegans Germline Stem Cells Under Nutritional and Metabolic Stress
<p>Stem cells are integral for tissue maintenance and fertility. Therefore, understanding how stem cells are regulated under stress is imperative. When confronted with acute starvation, stem cells must conserve energy and metabolites to cope with the lack of an external source. <em>Caenorhabditis …
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Mitotic cell cycle progression and differentiation of germline stem cells in Caenorhabditis elegans
In the C. elegans germline, stem cells make a decision to retain the germline proliferative cell fate or differentiate by entering meiosis. Importantly, this decision must be coordinated with progression through the mitotic cell cycle. Previous work has shown that the conserved GLP-1/Notch …
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Drosophila Cyclin J And The Somatic Pirna Pathway Cooperate To Regulate Germline Stem Cells
… egg chambers containing an overabundance of germline cells, while double mutants of CycJ and piwi or armi had more severe defects. CycJ mutants also genetically interacted with mutants of the germline piRNA pathway member aub, resulting in unique oogenesis defects, though they were less …
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Differentiation of Flk-1 positive multipotent adult germline stem cells into endothelial cells in vitro and in vivo
… und in vivo gezeigt. Zu Beginn wurde ein Kultursystem zur Differenzierung Flk-1 positiver, von maGSC-stammenden Zellen in funktionale ECs etabliert. Diese differenzierten maGSC-ECs wiesen typische endotheliale Morphologien und Genexpressions-Muster auf. Außerdem zeigten sie Proliferations- und …
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Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in <i>Caenorhabditis elegans</i> (roundworm) germline stem cells
… the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting …
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The Role of Hiwi in Stem Cell Maintenance and Sarcomagenesis
… of the mesenchyme. As such, mesenchymal stem cells are thought to be the cell of origin for sarcomas. Genetic or epigenetic lesions at particular points during the differentiation of a mesenchymal stem cell into its terminal mesenchymal cell type are able to give rise to specific subtypes …
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Characterizing the Role of Spegless, a Novel Inner Nuclear Membrane Protein required for spermatogenesis and oogenesis in Drosophila melanogaster
… process regulated by the interplay between germline stem cells and somatic (cyst) stem cells. Sperm develop in units called cysts and flies lacking speg had small testes that lacked proper cyst structure and cyst cell membrane markers. Speg is important in the cyst cells as cyst cell …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
Specialized microenvironments called niches keep stem cells in an undifferentiated and self-renewing state by producing a variety of factors. The size and signaling output of niches must be finely tuned to ensure proper tissue homeostasis. I use the Drosophila female germline as an excellent model …
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Zur Rolle von Stra8 in pluripotenten Stammzellen
… Stammzellen)- und maGSC (multipoten! t adult Germline Stem Cells)-Zelllinien nachgewiesen werden. Dabei zeigte sich, dass Stra8 in diesen Zellen zum Teil zytoplasmatisch, aber auch intranukleär lokalisiert ist. Es konnte zudem gezeigt werden, dass die Stra8-Expression in pluripotenten …
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Identification of a novel autonomous role for Ecdysone Receptor during Drosophila ovarian germ cell differentiation
… signals coordinates the division of Drosophila germline stem cells (GSCs) and differentiation of their daughter cells to produce viable oocytes. The steroid hormone, ecdysone, is known to promote GSC self-renewal; however, due to lack of compatible genetic tools, potential autonomous roles in …
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Investigating the coordination of germline stem cell fate by ecdysone signaling and BMP signaling in the Drosophila ovary
Germline stem cells (GSCs) receive multiple secreted signals that regulate their fate. How these signals are integrated to balance self-renewal with differentiation are not well understood. Here, we use the Drosophila ovary as a model to understand how signal convergence is achieved. Ecdysone …
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In Vivo Studies of Ribosomal RNA Transcriptional Regulation within a Drosophila Stem Cell Lineage
… transcription is differentially regulated in cells within the same lineage during differentiation in vivo and how changes in rRNA levels affect cell fate determination remains unclear. Here I present the discovery and characterization of a novel Drosophila RNA polymerase I transcriptional …
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Rbfox1 Regulates mRNA Translation to Promote Germ Cell Differentiation
Germ cells are the only cells that can give rise to an embryo. During differentiation, female germ cells that will give rise to oocytes form a syncytium called a germline cyst. The mechanisms that regulate germline cyst development remain poorly understood. In Drosophila, germline stem cells (GSCs) …
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microRNA expression profile of undifferentiated and differentiating pluripotent cells
… von differenzierten maGSCs (multipotent adult Germline Stem Cells-multipotente adulte Keimbahn-Stammzellen) im Vergleich zu differenzierten ESCs (Embryonic Stem Cells-embryonale Stammzellen) in der Maus. Dazu wurden globale miRNA Array Expressionsprofile von nicht differenzierten und …
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Studying the Interaction of RACK-1 and GLD-1 in the Germline of Caenorhabditis elegans
Stem cells are undifferentiated cells that can proliferate to produce more stem cells or differentiate to produce specialized cells. Two gonad arms are present within the germline of the hermaphrodite C. elegans, where proliferating germline stem cells (GSCs) are located at the distal end while …
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Ubiquitin Conjugase UBE2K Is Essential for Normal Rat Development and Spermatogenesis
… questions about human physiology using in vivo systems. Following the advent of gene targeting by homologous recombination in mouse pluripotent embryonic stem cells during the early 1980's [Reviewd by (Hamra, 2010)], the laboratory mouse has emerged as the most widely published animal model in …
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Characterization of germ cell development in free-living and parasitic flatworms
… correct specification and maintenance of germ cells for species survival. Germ cells must be carefully protected from inappropriate differentiation while simultaneously maintaining their identity as highly specialized totipotent cells. Several germ cell-intrinsic mechanisms, including …
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Chromosome evolution and mechanisms of speciation in the Anopheles gambiae complex
… An. gambiae complex provide an excellent model system for these topics. To understand the mechanisms of speciation, we investigated the cellular basis and phenotypes of hybrid male sterility in species crosses of the An. gambiae complex. By performing inter-species crosses of An. coluzzii/An. …
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Isolation, characterisation and in vitro potential of Oogonial stem cells
… and subsequent isolation, of purported oogonial stem cells (OSCs) from adult mammalian ovaries. This rare cell population has now been reported in the mouse, rat, pig, rhesus macaque monkey and humans and, although a physiological role for the cells has not been proven, they do appear to generate …
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