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Showing 1 to 12 of 12 for “"Geobacillus stearothermophilus"”.
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Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.
… was discovered in the thermophilic bacterium <em>Geobacillus stearothermophilus</em> strain 10 (Vellore <em>et al.</em>, 2004, Appl. Environ. Microbiol. <em>70</em>: 7140-7147). Numerous copies of the intron, designated Gs. Int1, are present in the chromosome of strain 10 but absent from a related …
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Effect of Ultrasonication on Biofilm Forming Ability of Common Dairy Sporeformers
… sporeformers. Three different bacteria, <em>Geobacillus stearothermophilus</em> (ATCC®15952), <em>Bacillus licheniformis</em> (ATCC® 6634), and <em>Bacillus sporothermodurans</em> (DSM 10599) were selected for this study based on our previous experiments. Skim milk was inoculated with a …
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Geobacillus genties bakterijų genetinės transformacijos sistemos kūrimas /
Thermophilic bacteria of genus Geobacillus are valuable microorganisms that can be used in various biotechnological processes such as bioconversion and bioremediation. However, effective employment of geobacilli is impossible without a reliable and convenient methodology for their genetic …
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Development of genetic transformation system for bacteria of the genus Geobacillus /
Thermophilic bacteria of genus Geobacillus are valuable microorganisms that can be used in various biotechnological processes such as bioconversion and bioremediation. However, effective employment of geobacilli is impossible without a reliable and convenient methodology for their genetic …
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Structural Studies on the Regulation of Sporulation in Bacillus and Antibiotic Sulfation
… (1) Solved the X-ray crystal structure of a Geobacillus stearothermophilus sporulation histidine kinase in complex with an inhibitor of the kinase reaction (Chapter 2). (2) Developed an approach using the methods of protein semisynthesis and small molecule organic chemistry that will …
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Silaffin Tagged Polymerase for Nucleic Acid Amplification
… biology approach to deliver a recombinant Geobacillus stearothermophilus (BST) DNA polymerase capable of low-cost purification, ease-of-use, and compatible with sensitive and specific loop mediated isothermal amplification (LAMP) assays. Fusing the enzyme with a silaffin tag (R5) and a …
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Termofilinių bakterijų genetinė transformacija /
… three thermophilic bacteria strains – Parageobacillus toebii DSM14950T, Geobacillus stearothermophilus 15 ir Aeribacillus pallidus 8. Development of transformation system for the latter two strains was based on in vivo methylation by expressing methyltransferase genes in Escherichia coli …
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Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… proteins G6PDH-DI and DI-G6PDH were cloned from Geobacillus stearothermophilus, heterologouely expressed in E. coli and purified through its His-tag. The individual proteins G6PDH and DI have good thermostability and reactivity. However, the presence of DI in fusion proteins drastically decreased …
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Enumeration and discrimination of thermophilic bacilli in milk powder
… of the milk powders screened. The thermophilic Geobacillus stearothermophilus strain A represented the third most often occurring milk powder isolate. The presence of B. licheniformis strain G, Bacillus subtilis and A. flavithermus strain D as low-level contaminants of milk powder was …
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Sequestration of metal and metalloid ions by thermophilic bacteria
… Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, and Thermus were determined. The highest tolerances to cadmium ions (Cd2+) in the range of 400 to 3200 micro;M were observed for species belonging to the genus Geobacillus. The thermophilic Gram-positive bacteria Geobacillus …
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Harnessing the optical properties of gold nanorods: fluorescence enhancement, biosensing and photothermal therapy
… vegetative cells and endospores of the bacterium Geobacillus stearothermophilus. Gold nanorods are prepared and characterized and are coated with four different neutral or charged polymers to investigate the impact of surface charge on cell attachment and inactivation. The effects of gold nanorod …