Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 28 for “"Geobacillus"”.
-
Geobacillus genties bakterijų genetinės transformacijos sistemos kūrimas /
Thermophilic bacteria of genus Geobacillus are valuable microorganisms that can be used in various biotechnological processes such as bioconversion and bioremediation. However, effective employment of geobacilli is impossible without a reliable and convenient methodology for their genetic …
-
Engineering geobacillus thermoglucosidasius for efficient production of fuels and chemicals
… this thesis, we aimed to develop the thermophile Geobacillus thermoglucosidasius as a cell factory for fuels and chemicals production from renewable biomass. G. thermoglucosidasius is a facultatively anaerobic thermophilic bacterium growing between 37°C to 70°C, and can ferment diverse …
-
Development of genetic transformation system for bacteria of the genus Geobacillus /
Thermophilic bacteria of genus Geobacillus are valuable microorganisms that can be used in various biotechnological processes such as bioconversion and bioremediation. However, effective employment of geobacilli is impossible without a reliable and convenient methodology for their genetic …
-
The crystal structure of an aliphatic amidase from Geobacillus pallidus RAPc8
… of acidic products. Aliphatic amidase from Geobacillus pallidus RAPcS (RAPcS amidase), which belongs to the nitrilase superfamily of enzymes, has recently been characterised biochemically. It shows both amide hydrolysis and acyl transfer activities, and also exhibits stereo selectivity for …
-
Investigation of the determinants of thermal stability of the nitrile hydratase from Geobacillus pallidus RAPc8
… composite mutants generated from the wild type Geobacillus pallidus RAPc8 nitrile hydratase (NHase), namely: L103S+Y127N+F36L+D4G, M43K+T150A+S169R and D96E+D167V+M188V each labelled as 9E, 9C and 8C respectively. The composite mutants were previously developed using error-prone PCR of the wild …
-
Characterization of the Group II Intron Gs. Int1 from the Thermophilic Bacterium <em>Geobacillus stearothermophilus</em>.
… was discovered in the thermophilic bacterium <em>Geobacillus stearothermophilus</em> strain 10 (Vellore <em>et al.</em>, 2004, Appl. Environ. Microbiol. <em>70</em>: 7140-7147). Numerous copies of the intron, designated Gs. Int1, are present in the chromosome of strain 10 but absent from a related …
-
An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
… A novel moderately thermophilic microorganism, Geobacillus pallidus RAPc8, was isolated by our collaborators (Pereira and co-workers, 1998). The strain has an optimal growth temperature of 65oC and constutitively expresses a thermostable nitrile hydratase. The gene cluster containing the nitrile …
-
Synthesis of enantio-pure amides by reversal of the Geobacillus pallidus RAPc8 amidase hydrolysis reaction in non-aqueous media
… was previously isOlated from a thermophilic Geobacillus species, and the amidase was cloned and expressed in an Escherichia coli BL21 strain. Also in previous studies, it was shown that the enzyme exhibits both amide hydrolysis and acyl transfer activities. The highest activity of the G. …
-
Peptidazių iš Geobacillus thermoleovorans DSM 15325 kolagenolizinio aktyvumo analizė: M3 proteolizinių fermentų šeimos M3B pošeimio oligopeptidazės ir U32 proteolizinių fermentų šeimos peptidazės charakterizavimas /
… of collagenases by moderate thermophile Geobacillus thermoleovorans DSM 15325 confirms the ability of thermophiles to be ever ready for collagen uptake. The identification of constitutive collagenolytic peptidases variety produced by geobacilli lead to substantial development of collagen …
-
Sequestration of metal and metalloid ions by thermophilic bacteria
… Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, and Thermus were determined. The highest tolerances to cadmium ions (Cd2+) in the range of 400 to 3200 micro;M were observed for species belonging to the genus Geobacillus. The thermophilic Gram-positive bacteria Geobacillus …
-
The collagenolytic activity analysis of Geobacillus thermoleovorans DSM 15325 peptidases: characterization of M3 proteolytic enzymes family M3B subfamily oligopeptidase and U32 proteolytic enzymes family peptidase /
… of collagenases by moderate thermophile Geobacillus thermoleovorans DSM 15325 confirms the ability of thermophiles to be ever ready for collagen uptake. The identification of constitutive collagenolytic peptidases variety produced by geobacilli lead to substantial development of collagen …
-
Aerob-thermophile Reinigung mineralölkontaminierter Abwässer
… Bedingungen ist der thermophile Mikroorganismus Geobacillus thermoleovorans im Labor- und Pilotmaßstab fähig, verschiedene mit Mineralölen kontaminierte Abwässer als einzige Kohlenstoffquelle zu nutzen. Die Verwertbarkeit eines synthetischen Dieselöls sowie eines Kühlschmierstoffs …
-
Functional assignments in the enolase superfamily: investigations of two divergent groups of D-galacturonate dehydratases and galactarate dehydratase-III
… group of D-galacturonate dehydratases, the Geobacillus group of D-galacturonate dehydratases, and galactarate dehydratase-III from Agrobacterium tumefaciens strain C58. Although the two groups of D-galacturonate dehydratases produce an identical product, they act through different mechanisms …
-
Process integration in the optimisation of amidase production from recombinant Escherichia coli
… a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded bed adsorption (EBA) was assessed as a …
-
Effect of Ultrasonication on Biofilm Forming Ability of Common Dairy Sporeformers
… sporeformers. Three different bacteria, <em>Geobacillus stearothermophilus</em> (ATCC®15952), <em>Bacillus licheniformis</em> (ATCC® 6634), and <em>Bacillus sporothermodurans</em> (DSM 10599) were selected for this study based on our previous experiments. Skim milk was inoculated with a …
-
Discovery and evaluation of novel and characterised bacteriocins for future applications
… new antimicrobial candidates in the genus Geobacillus. This resulted in the discovery of the potentially novel bacteriocin thermocin 458, who’s amino acid sequence and molecular mass is unknown due to an interesting inability to generate enough peptide for in depth physicochemical …
-
Structural Studies on the Regulation of Sporulation in Bacillus and Antibiotic Sulfation
… (1) Solved the X-ray crystal structure of a Geobacillus stearothermophilus sporulation histidine kinase in complex with an inhibitor of the kinase reaction (Chapter 2). (2) Developed an approach using the methods of protein semisynthesis and small molecule organic chemistry that will …
-
Silaffin Tagged Polymerase for Nucleic Acid Amplification
… biology approach to deliver a recombinant Geobacillus stearothermophilus (BST) DNA polymerase capable of low-cost purification, ease-of-use, and compatible with sensitive and specific loop mediated isothermal amplification (LAMP) assays. Fusing the enzyme with a silaffin tag (R5) and a …
-
The structure, function and engineering of a thermostable nitrile hydratase
… this study belongs to the thermophilic bacteria Geobacillus pallidus. The G. pallidus RAPc8 NHase is a heterotetramer that has a 28 kDa α subunit and a 29 kDa β subunit, with a α2β2 configured functional unit. The G. pallidus RAPc8 NHase operon has been cloned, sequenced and expressed at high …
Page 1 of 2