Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Genotyping-by-sequencing (GBS)"”.
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ASSOCIATION MAPPING OF GENE REGIONS FOR DROUGHT TOLERANCE AND AGRONOMIC TRAITS IN SORGHUM
… the genetic architecture of these traits by employing a novel multi-parent advanced generation intercross (MAGIC) population. The population was formed from 19 founder lines through ten generations of random mating, aided by genetic male sterility (GMS) system. This was followed by seven …
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Population Genetic Structure of Unionid Mussels Across Multiple Gulf Drainages
… management decisions. This study utilized a genotyping by sequencing (GBS) approach to study the genetic structure of three Texas state threatened mussel species of family Unionidae: Pleurobema riddellii (Louisiana Pigtoe), Fusconaia askewi (Texas Pigtoe), and Potamilus amphichaenus (Texas …
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Assessment of a Muscadine Grape (Vitis rotundifolia) Mapping Population for Tolerance to Pierce’s Disease Pathogen Xylella fastidiosa and Resistance to Black Rot Disease Pathogen Guignardia bidwellii
… genes is crucial for controlling these disease by developing resistant cultivars. Muscadine grape (<em>Vitis rotundifolia</em>) cultivar ‘Pam’ has been identified to possess a high level of resistance to diseases. To elucidate the genetic basis of the resistance to the causal pathogens …
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Linkage mapping in prairie cordgrass (Spartina pectinata Link) using genotyping-by-sequencing markers
… in prairie cordgrass are currently hampered by the lack of a linkage map, the lack of a Chloridoid reference genome, and by lack of information on inheritance patterns (disomic versus polysomic). Genotyping-by-sequencing (GBS) was applied to a population of 85 progeny from a reciprocal cross …
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Molecular Marker Applications in Oat (Avena Sativa L.) Breeding and Germplasm Diagnostics
… these inconsistencies, and with advances in genotyping technology these diagnostics can be done faster and more objectively. This study aimed to develop molecular marker assays and graphical genotyping methodologies for cultivar identification, seed purity assessment and trait selection in …
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GENOMICS FOR WILD AND MEDICINAL PLANT DIVERSITY STUDIES
… Genetic variation data was obtained with Genotyping-By-Sequencing (GBS). In the second part of the project, in collaboration with Aboca S.p.A. Società Agricola, we set the basis for future studies aimed at improving Aboca’s collection of Althaea officinalis, a medicinal plant used for …
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Background-dependent genetic architecture of Fusarium head blight resistance in U.S. hard winter wheat
Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is one of the most destructive diseases of wheat worldwide, causing substantial yield losses and mycotoxin contamination of grain. Deployment of major resistance quantitative trait loci (QTL) through marker-assisted breeding is …
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Genetic dissection of sorghum height and maturity variation using sorghum converted lines and their exotic progenitors
… partially-isogenic populations were generated by crossing six diverse SC lines to their corresponding exotic progenitor (EP) lines to assess the phenotypic effects of individual introgressions from the temperate-adapted donor. Initial genotyping results revealed one of the six populations …
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A sorghum NAC gene is associated with variation in vascular development, biomass properties, and yield potential
… (NILs) were created, and flanking markers and genotyping-by-sequencing (GBS) were applied to BC3 and BC2F2 individuals to reduce the region of interest to a 38 kb interval containing 4 candidate genes. One of these genes is a NAC gene containing a stop codon mutation in the mutant (dd) allele. …
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Linkage disequilibrium and haplotype block patterns in popcorn populations
… population). Three populations were genotyped by genotyping-by-sequencing (GBS), 190 or 192 individual from each population. After the data quality control the final marker set remaining for analysis ranged from 75,000 to 76,055 SNPs. D’ and r 2 were assessed per chromosome, and haplotype …
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The genetic determinants of vigour control and precocity by pear (Pyrus communis L.) rootstocks
… control and early flowering conferred to a scion by Pyrus rootstocks. A segregating population of 421 F1 seedlings from a P. communis ‘Old Home’ x ‘Louise Bonne de Jersey’ (OHxLBJ) cross was grafted with clonal ‘Doyenne du Comice’ scions and used as the core experimental material for this project. …
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Sweet sorghum diversity, genetics, and breeding
Sweet sorghum is characterized by high sugar content and high biomass and is of growing interest to the biofuel industry. For sweet sorghum to be successfully used for bioenergy production, genetic relationships and diversity within sweet sorghums should be better understood, genetic and …
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Developing Low-GN and GN-zero Winter Malt Barley for the Eastern United States
… (GN) are carcinogenic precursors produced by barley as a natural defense against insects. The most common GN in barley, epiheterodendrin (EPH), contains a cyanide group that, when combined with heat and copper during distillation, forms ethyl carbamate (EC), a known carcinogen. While …
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Genomic Prediction and Genetic Dissection of Yield-Related Traits in Soft Red Winter Wheat
… polymorphism (SNP) data generated via a genotyping-by-sequencing (GBS) protocol to perform GWA on multiple yield-related traits using a panel of 329 soft red winter wheat genotypes grown in four environments. In addition, the SNP data was used to examine linkage disequilibrium and …
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Genomic selection and quantitative trait loci mapping for Fusarium head blight resistance in wheat (Triticum aestivum L.)
… growing areas. The disease is primarily caused by Fusarium graminearum Schwabe [telemorph: Gibberella zeae Schw. (Petch)], in North America, and the majority of current wheat cultivars are susceptible to it. Significant economic losses are associated with FHB since it results in yield reduction, …
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Navigating through the uncertainty of genotyping-by-sequencing data in polyploids
The development of genotyping-by-sequencing (GBS) methods has facilitated genomics studies in non-model species, including polyploids. Variant and genotype calling methods have been established for autopolyploids but for a species with a complex genome, such as sugarcane, the level of uncertainty …
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Combining and mapping QTL for fusarium head blight (FHB) resistance in wheat
… also the disease severity is highly influenced by environment. Consequently multiple genes are required for effective resistance. Our hypothesis is that identifying DNA markers for type I resistance will be very beneficial for selection, and combining type I and type II FHB resistance will be …
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Genomic characterization of multi-parental populations of sorghum and rice
… recombinant inbred line (RIL) populations using genotyping-by-sequencing (GBS) data. In this work, 1626 RILs from eight biparental populations were genotyped using a modified GBS protocol with Y-adapters. For SNP calling used the TASSEL5 GBS pipeline, and genetic linkage maps were produced using …
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Molecular mapping of quantitative trait loci (QTL) for Fusarium head blight (FHB) resistance and agronomic traits in US hard winter wheat
… trait loci (QTL) in US hard winter wheat by genotyping the populations with single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) and phenotyping the FHB and agronomic traits in both greenhouse and field experiments. In the G97252W x G97380A RIL …
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Computational methods for genomic variant calling and analysis
The development of short-read, next-generation sequencing (NGS) has revolutionized biological research, agriculture and medicine, enabling innovations such as genomic selection to raise crop yields and precision medicine to diagnose and treat disease. The genetic polymorphisms identified by this …
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