Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Genotoxic stress"”.
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Determination of genotoxic stress in feral populations of bullheads and carp using the alkaline single cell gel assay.
Monitoring genotoxicity of the environment using endemic organisms as sentinels requires the development of sensitive assays. Towards this end, I explored the feasibility of applying the ALKALINE SINGLE CELL GEL (SCG) or "COMET" ASSAY. This approach involves detection, under alkaline conditions, of …
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Modulation of Clr-b Ligand for the Inhibitory NKR-P1B NK Cell Receptor in Response to Transformation and Genotoxic Stress
… receptors. The recognition of targets undergoing genotoxic stress or transformation by NK cells has been limited to the upregulation of stimulatory NKG2DL and “missing-self” recognition of MHC-I. The ligand pair NKR-P1B:Clr-b participates in “missing-self” recognition of tumour and infected cells, …
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THE ROLE OF INTERFERON REGULATORY FACTORS IN REGULATING THE EXPRESSION OF NKG2D LIGANDS
… damage response (DDR) is a cellular response to genotoxic stress that triggers cell cycle arrest and DNA repair mechanisms. It has been previously shown that NKG2D ligands are upregulated by the DDR in a p53-independent manner. We provide evidence in this study that the upregulation of NKG2D …
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Replication fidelity in the mircroevolution of mycobacteria
… PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected …
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The Role of Nucleolin Phosphorylation by CK2 in Regulating Cellular Fate Under Normal and Stress Conditions
<p>Nucleolin (NCL or C23) is an abundant genotoxic stress-responsive RNA binding phosphoprotein. NCL constitutes 10% of total nucleolar protein that has functions in multiple biological processes, including ribosome biogenesis, DNA/RNA metabolism, cellular response to DNA damage, cell growth, …
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Defining the functions of a conserved hydrophobic domain in the ARF tumor suppressor
… levels, but under conditions of oncogenic stress, p19Arf expression is induced and its tumor suppressive activities are mediated through the stabilization of p53 or in a p53-independent manner. Introducing a point mutation (L46D) into the conserved hydrophobic domain (37-51) in p19Arf …
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Finding NEMO by the molluscum contagiosum virus MC159 protein and its effect on viral pathogenesis in vivo
… MC159 can inhibit NF-kB activation triggered by genotoxic stress. This signal transduction pathway has some unique features that are missing during TNF-induced NF-kB activation. I used mutational analysis of MC159 to identify that there are some regions of MC159 that are important for protecting …
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Insights on the Regulation of the PERIOD 2 Gene in the Cellular Response to DNA Damage
… shed light on the regulation of PER2 under genotoxic stress, genetic variations of Per2 in normal human population, and expression of circadian genes under iron controlled diets.
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Molecular mechanisms of DNA repair in Mycobacterium tuberculosis
… DNA polymerase, DnaE2, in mutagenesis under stress and, secondly, the function of the mycobacterial homologue of a putative SOS response associated peptidase (SRAP) protein which has been identified in comparative genomics analyses of organisms possessing a DnaE2-type C family DNA polymerase. …
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Interlocking mechanisms regulating the circadian clock response to DNA damage
… cycle progression and the cellular response to genotoxic stress. PER2 binding to p53 modulates p53's stability, cellular localization, and transcriptional activity. As described in Chapter 2, we now identified PER2 as a previously uncharacterized substrate for the ubiquitin E3 ligase mouse …
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Investigating The Role of Abro1 In Dna Damage-Induced Immune Response
… instability can be induced by various forms of genotoxic stress and it is a hallmark of human cancer that is associated with metastasis, therapeutic resistance, and poor prognosis. During replication stress, the replication fork stalls and forms a reversed fork which may be degraded by several …
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OTULIN's Novel Regulatory Mechanisms in Genotoxic and Inflammatory NF-kB Signaling
… facilitate NF-kB signaling transduction upon genotoxic stress. OTU deubiquitinase with linear linkage specificity (OTULIN) is known to cleave linear ubiquitin chains exclusively. The purpose of this dissertation is to investigate whether OTULIN can counteract LUBAC-mediated NF-</p>
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The functional and molecular characterisation of the novel DNA damage repair protein Bod1L
… in undamaged cells and hypersensitivity to genotoxic stress. Bod1L binds to chromatin and is phosphorylated by ATM at sites of DNA damage. In Bod1L-depleted cells, ATM activity is normal, but there is over-accumulation of MDC1 at damaged sites caused by hyper-phosphorylation of MDC1-T4. I …
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Enzymatic synthesis of defined-length poly(ADP-ribose) and the investigation of cell-permeable poly(ADP-ribose) glycohydrolase inhibitors
… gathered considerable interest as a target for genotoxic chemotherapy against cancer cells. To this end, various Poly(ADP-ribose) Polymerase (PARP) inhibitors have been developed to induce sensitivity to genotoxic stress in BRCA-mutated cancer cells, and Poly(ADP-ribose) Glycohydrolase (PARG) …
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p53 in a Genetic Model: Illuminating Adaptive Radiation Responses
When cells are challenged by genotoxic stress, the tumor suppressor protein p53 promotes adaptive responses, including cell cycle arrest, DNA repair, or apoptosis. How these distinct fates are specified through an action of a single protein is not known. To study its functions in vivo we produced a …
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Mechanisms of apoptosis modulation and their contribution to genomic instability in tumor cells
… genomic damage and induction of apoptosis under genotoxic stress. After genotoxic insult there are different possibilities for the fate of a cell (Figure 1). The genomic integrity is analyzed at cellular checkpoints, usually leading to a delay in cell cycle progression if DNA was damaged. …
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Novel Role of CHK2 in the Intrinsic and Extrinsic Apoptosis Pathway
<p>In response to genotoxic stress, cells activate DNA damage checkpoint pathways that maintain genetic fidelity, promote cell survival, and prevent malignant transformation. Checkpoint kinase 2 (CHK2) is an evolutionarily conserved serine/threonine protein kinase that is activated in response to …
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ATM REGULATES THE NF-kB PATHWAY VIA RELA SER 276 PHOSPHORYLATION
… the double strand DNA break-repair pathway after genotoxic stress. Here we found ATM serves as an essential regulator of TNF-and RSV- induced NF-kB pathway. We observed that TNF exposure of cells rapidly induced DNA double strand breaks and activates ATM. TNF-induced ROS promote nuclear …
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