Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 95 for “"Genomic stability"”.
-
Mechanisms of genomic stability maintenance in fission yeast
… related to increased copies of specific genomic segments are the Copy Number Gains (CNGs). In order to establish a cause and effect relationship between re-replication and CNGs, an inducible cdc18 overexpressing cell strain was exploited. Re-replication was induced and the incidence of …
-
Discovering GCNA: A Novel Regulator of Germline Genomic Stability
… (GCNA) as a conserved regulator of genome stability across multiple species. Loss of GCNA results in replication stress, chromosomal instability, and an accumulation of DNA-protein crosslinks (DPCs). Disruption of GCNA leads to an accumulation of nuclear Top2 and Top2 DPCs. This work shows …
-
Novel Roles for A-Type Lamins in Maintaining Genomic Stability
… nuclear organization affects genome function and stability and the relationship between nuclear organization, cancer and other age-related diseases. To advance the field, I have investigated the role of A-type lamins in the maintenance of genomic stability in mammalian cells. I present data …
-
Single-molecule study of the DNA helicase regulating genomic stability
A DNA helicase, PcrA is an essential protein in gram-positive bacteria. PcrA utilizes its helicase activity and translocation activity to regulate various cellular functions including plasmid replication and counteracting deleterious recombination. Using single molecule FRET technique and …
-
Fancm and Faap24 Maintain Genomic Stability Through Cooperative and Unique Functions
… the functions of FANCM and FAAP24 inchromosome stability, FA pathway activation, DNA damage checkpoint signaling, and ICL repair. I found that the<em> FANCM<sup>-/-</sup>/FAAP24<sup>-/- </sup></em>double mutant was much more sensitive to DNA crosslinking agents than <em>FANCM<sup>-/-</sup></em> …
-
DNA crosslink repair safeguards genomic stability during pre-meiotic germ cell development
… importance that germ cells maintain the stability of their genome. Failure to do so can result in the transmission of de novo mutations to offspring. Germline de novo mutations are the basis of genetic disease and the foundation of genetic diversity within a species, and the substrate on …
-
In Vitro Kavapyrone Production and Genomic Stability of in Vitro Kava Cultures
Inter simple sequence repeat (ISSR) markers were used for detection of polymorphism among in vitro kava cultures. An absence of normally observed bands was observed among organogenic callus cultures, while a small degree of ISSR polymorphism was detected among regenerated plantlets.
-
Novel Structures of RAD51 Reveal Mechanisms in DNA Damage Repair and Genomic Stability
… RAD51 protein contributes to the maintenance of genomic stability by promoting the repair of DNA double-strand breaks and the protection of DNA replication forks. RAD51 functions alongside the tumour suppressor protein BRCA2 to catalyse DNA strand-exchange reactions which form an integral part of …
-
Long-term expansion, genomic stability, in vivo safety and immunogenicity of adult human pancreas organoids
… diseases and the future development of a genomically stable replacement cell therapy for diabetes mellitus. hPOs can be generated and expanded in a chemically defined, serum-free, human pancreas organoid culture medium with high efficiency from both fresh and cryopreserved primary tissue. …
-
Fluorescent detection of homologous recombination reveals the impact of genetic, physiological, and environmental factors on genomic stability
Unless repaired correctly, DNA double strand breaks (DSBs) can cause the loss of millions of base pairs of information and can induce cellular toxicity. DSBs are repaired via mitotic homologous recombination (HR), non-homologous end-joining (NHEJ) or microhomology-mediated end-joining (MMEJ). Here …
-
Uncovering vital molecular contacts within the replisome and characterizing fail-safe mechanisms ensuring genomic stability in E. coli.
… rolling circle experiments, we created analogous genomic mutants of α-Pol III in vivo using CRISPR-Cas9. Our in vivo investigations uncovered severe cellular and genomic fitness deficits. Decoupling between enzymes led to severe cellular stress phenotypes, characterized by reduced growth, lower …
-
Clamp loading on the leading strand during DNA replication and initial reconstitution of sister chromatid cohesion establishment
… dysregulation of this vital process resulting in genomic instability and disease. In eukaryotes this process is performed by the replisome, a complex protein machinery composed of a core helicase, DNA polymerases, and a wide range of replisome-associated factors. One such factor is the sliding …
-
MenzelJohannes_MSc_July_2013
… scrutiny in recent years. It is clear that genomic stability, stress response and nutrient signaling all play critical roles in lifespan determination, but the precise molecular mechanisms and their often subtle influence on cellular function remain largely unknown. The Anaphase Promoting …
-
Analysis of Chromosomal Rearrangements in Host Specific Salmonella Serovars
… It is possible that the discrepancy in genomic stability between the serovars is due to variations in the lifestyle of these organisms and not an inherent difference in their recombinational machinery.
-
The Role of the RNA-binding Protein ZFP36L1 in Suppression of Replication Stress-induced Genomic Instability
… and the consequent failure to preserve genomic stability. ZFP36L1, a member of the ZFP36 family of CCCH tandem zinc finger (ZF) proteins, is an RNA-binding protein that plays a crucial role in the post-transcriptional regulation of gene expression underlying physiological processes and …
-
Understanding The Roles of Non-Homologous End Joining and P53 After Dna Damage
<p>The inability to maintain genomic stability and control proliferation are hallmarks of many cancers, which become exacerbated in the presence of unrepaired DNA damage. Such genotoxic stresses trigger the p53 tumor suppressor network to activate transient cell cycle arrest allowing for DNA …
-
Flap Endonuclease 1 ensures telomere replication and stability
… fide DNA double strand break, are integral to genomic stability. High fidelity replication of telomeres is indispensable for their stability. Telomere replication is a challenging task that is completed through the coordinated actions of telomere binding proteins and DNA replication and repair …
-
Roles of RNA interference and DNA mismatch repair in maintaining genomic integrity in Cryptococcus pathogens
<p>Microorganisms must regulate genomic stability to strike a balance between excessive deleterious mutation and evolutionary stagnation to successfully compete and endure within their ecological niches. Two important mechanisms involved in maintaining genomic stability are RNA interference (RNAi) …
Page 1 of 5