Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Genomic sequencing"”.
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Linkage mapping in tetraploid blackberry (Rubus spp.) using high-throughput genomic sequencing and restriction-site associated DNA sequencing (RAD-seq)
Genomic molecular marker research and linkage mapping in cultivated polyploid species of Rubus, in particular blackberry, has lagged behind diploid members of the genus due to the complex analyses required to deduce allele dosage among homologous and homeologous chromosomes. Traditional techniques …
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Enabling sample tracking in and reducing variability in DNA library construction
The Broad Institute is a world leader in genomic sequencing. The Institute gained prominence during the Human Genome Project and has continued to be the nation's leading public laboratory for genomic sequencing projects. The Broad Institute has done this by taking advantage of economies of scale, …
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Molecular genetic investigations of renal cell carcinoma predisposition
… be uncovered. This study has utilised a range of genomic sequencing methodologies, scaling from single gene to whole genome sequencing, on individuals with features of renal cell carcinoma predisposition in order to identify novel causes of heritability associated with RCC. Multiple genomic …
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World to Word: Nomenclature Systems of Color and Species
… integrative approaches to taxonomy in biology, genomic sequencing identifies one or few quantitative characteristics to encode an organism. If genomic sequencing becomes the primary means of identification in the biological sciences, just as in numeric systems of color identification, scientific …
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On the use of locality aware distributed hash tables for homology searches over voluminous biological sequence data
Rapid advances in genomic sequencing technology have resulted in a data deluge in biology and bioinformatics. This increase in data volumes has introduced computational challenges for frequently performed sequence analytics routines such as DNA and protein homology searches; these must also …
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Haemobartonella Felis (Mycoplasma Haemofelis) Molecular Diagnostics and Genomic Studies
… to create a large insert library of the H. felis genomic DNA. Using hybridization experiments and fingerprinting analysis, these BACs were ordered into a genomic encyclopedia with a restriction map. The BACs were then sub-cloned into a plasmid to generate high throughput genomic sequencing surveys …
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An efficient score alignment algorithm and its applications
… that have practical application in bioinformatic genomic sequencing and data comparison in revision control systems. In the field of musicology, score alignment and comparison is a problem with many similarities to string comparison and alignment but also vast differences. In particular we can use …
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Coordination of Carbon Dioxide and Nitrogen Metabolism in Rhodobacter sphaeroides
… strains developed that deregulated nitrogenase. Genomic sequencing and comparative analyses of these mutant strains revealed multiple mutations that could account for the nitrogenase active phenotype. A mutation in the gene that encodes for glutamine synthetase and another in the gene that …
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Assessing the Role of Clusters Derived from Large Sequence Similarity Networks for Gene Function Predictions
Large scale genomic sequencing efforts have resulted in a massive inflow of raw sequence data. This raw data, when appropriately processed and analyzed, can provide insight to a trained biologist and aid in hypothesis-driven research. Given the time and resource requirements necessary for …
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Statistical foundations for precision medicine
… these concepts. HCM has proven tractable to genomic sequencing, which guides risk stratification for family members and tailors therapy for some patients. However, these benefits carry risks. I show how genomic misclassifications can disproportionately affect African Americans, amplifying …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… the plant kingdom are being discovered with new genomic sequencing technologies but, many of them remain uncharacterized. Recently, our lab discovered a new RIP called Novel PAP/KPAP in the sequenced pokeweed genome. KPAP transcript levels did not behave the same way as other isoforms under …
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Content Analysis of Consent Forms for Clinical Whole Exome Sequencing
As genomic sequencing becomes increasingly incorporated into clinical care, the patient informed consent process must successfully manage many ethical challenges, including whether to seek secondary findings and which results will be returned to the patient. The goal of the current study was to …
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Identification of echinus and characterization of its role in Drosophila eye development
… deletion mapping, gene expression analysis and genomic sequencing, echinus was cloned and several alleles were sequenced. echinus encodes a ~180kDa protein containing an ubiquitin hydrolase domain at its N-terminus and a polyglutamine tract at its C-terminus. echinus is expressed in the retina …
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A study of the ecology & diversity of bacteriophages
… found them to be highly related. As a result genomic sequence and hybridisation experiments were applied in order to quantify the degree of relatedness between phages. Genomic sequencing contributed to our understanding of the mechanisms involved in viral evolution within a single soil sample …
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Signatures of selection in the red fox (Vulpes vulpes) in Europe using genotype-by-sequencing
… selection, genetic drift, and gene flow. Using genomic sequencing methods such as genotype-by-sequencing (GBS) to detect single nucleotide polymorphisms (SNPs) can lead to a better understanding of a species evolutionary history and identify patterns of local adaptations. Abiotic and biotic …
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Genomic and Proteomic Characterisation of the European House Dust Mite, Dermatophagoides pteronyssinus
… house dust mites has been hampered by a lack of genomic sequence data and limited to a discrete number of proteins. The work presented here is a detailed characterisation of the European house dust mite, Dermatophagoides pteronyssinus airmid strain, at the genomic and proteomic level. Genomic …
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Leveraging knowledge networks for precision medicine
Akin to the exponential growth of genomic sequencing data, high-throughput techniques in proteomics and biotechnology have been creating ever-expanding repositories of proteomic, pharmacological, and interactomic data. Other molecular data, including expression profiles, genomic mutations and cell …
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Somatic Mutations in Primary Sjögren’s Syndrome
… disease, we performed targeted and whole genome sequencing of glandular epithelial cells and tissue-infiltrating T and B lymphocytes from biopsies of minor salivary glands from PSS patients and controls. The results illustrate the mutational trends and clonal dynamics present in immune cells and …
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Population genomics of Sulfolobus islandicus: genome-wide recombination and antagonistic interactions
… and recombination. With the onset of the era of genomic sequencing of natural populations of microorganisms, it has been revealed that genetic recombination is not just a useful laboratory tool for microbial geneticists, but instead a prominent feature of microbial population biology. Conceptual …
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DNA Canvas: Towards affordable and scalable enzymatic fabrication of DNA nanoarrays
… for a variety of applications, from whole genomic sequencing at a fraction of the cost to sustainable digital information storage. Yet, due to the stochastic nature of self-assembly, DNA origami-based approaches suffer from an inherent scale limit. Top-down fabrication techniques enable …
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