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Showing 1 to 20 of 59 for “"Genomic library"”.
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Cloning of actin genes from a genomic library of the newt, Notophthalmus viridescens
… developmentally regulated genes from a newt genomic library for use in future studies of gene expression during limb regeneration. We decided to clone the cytoskeletal actin gene for the following reasons: 1. its expression reflects the proliferative and differentiatlve states of cells in …
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Gene Inhibition Using Genetic Suppressor Elements in Escherichia Coli JM109DE3 Cells
… from a single gene or isolated from a multigene library consisting of short random fragments of the target gene or genes. The goal of this study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to …
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Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene
… dismutase (SOD) was isolated from a LambdaGEM-11 genomic library of <i>Bacteroides fragilis</i>. In order to generate a complete genomic library, <i>B. fragilis</i> genomic DNA was partially digested with the restriction endonuclease Sau3AI and was ligated to cloning vector, LambdaGEM-11. After in …
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Genetic and biochemical studies of Vibrio alginolyticus glutamine synthetase
A genomic library of the collagenolytic Vibrio alginolyticus strain was established in Escherichia coli HB101 employing the positive selection vector pEcoR251. A glutamine synthetase (GS) gene, glnA was identified by complementation of the glnA deletion in E. coli ET8051 glnA, glnL, glnG deletion …
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Characterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… thermophila</i> were isolated from a genomic library on a fifteen kilobase fragment The genes are located adjacent to one another, with the phosphotransacetylase gene (<i>pta</i>) directly upstream of the acetate kinase gene (<i>ack</i>). The two genes were sequenced, along with a …
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Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II
… of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\alpha$-$\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with …
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Biochemical and genetic analysis of a fission yeast ribonucleoprotein homologous to signal recognition particle
… the S. pombe RNA was cloned by screening a genomic library. Sequence analysis shows that the two RNAs are structurally similar. Disruption of the chromosomal copy of the single copy 7SL gene is lethal to the haploid yeast, demonstrating that the RNA is essential for cell viability. Since …
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Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
… encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. Comparison of the deduced anlino acid sequence with the N-terminaI sequence of the purified protein shows that the gene encodes an additional 34 N-terminal residues with properties characteristic of …
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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… sucrose utilization system, was isolated from a genomic library of v. alginolyticus. Plasmid pVSlOO was mapped and the origin of its insert determined by Southern blotting and DNA hybridization. The number and sizes of the polypeptide products encoded by plasmid pVSlOO were determined by …
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Structure and expression of nuclear genes encoding light-harvesting chlorophyll a/b-binding polypeptides of photosystem II in apple and soybean
An apple genomic library was constructed with lambda Charon 35 vector. The library was screened using a pea LHCP-II cDNA and an apple LHCP-II gene (AB10) was identified, subcloned and characterized. AB10 contains an open reading frame of 807 nucleotides coding for a precursor of 269 amino acids …
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Population substructuring in Schreibers' long-fingered bat (Miniopterus schreibersii) in South Africa
… direction of bat movement among the colonies. A genomic library was constructed for M. schreibersii, and was screened for (CA)0 and (GA)0 microsatellite repeats. Five novel, highly polymorphic loci were identified. These five loci, and an existing mammalian microsatellite locus, were amplified …
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Phenotypic and genotypic relationship between tabtoxin production and pathogenicity in Pseudomonas syringae pv. tabaci strain PTBR2.024
… and is prototrophic. A 3-kb fragment from a genomic library of the parent strain PTBR2.024 complemented both mutant phenotypes. This 3-kb fragment contains two open reading frames (ORF), ORF1 and ORF2, and two truncated ORFs, ORF3 and ORF4. The Tn5 insert in PTBR7.000 was mapped to ORF2, and …
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Isolation and Characterization of a Full Length Retrotransposon: CR1
… genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent …
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Isolation and sequencing of a genomic clone of ESI28 : an early salt-stress induced gene of the salt-tolerant wild wheatgrass Lophopyrum elongatum
A genomic sequence corresponding to a putative actin-depolymerizing factor (ADF) was isolated from a wheat (Lophopyrum elongatum) genomic library. The genomic clone, designated ESI28-G-5, codes for the ESI28 gene (early salt-stress induced). The 5$\sp\prime$-upstream sequence contains a putative …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
A genomic library of Ruminococcus flavefaciens FD-1 DNA was constructed using the Escherichia coli bacteriophage $\lambda$ vector $\lambda$DASH. A recombinant phage exhibiting activity against both Ostazin brilliant red-hydroxyethyl cellulose and carboxymethyl cellulose (CMC) was isolated. This …
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Molecular characterization and induction of a peach beta-1,3-glucanase gene
A peach genomic library of cultivar Stark Earligo was screened using a pea $\beta$-1,3-glucanase gene as a probe. Five clones containing $\beta$-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach $\beta$-1,3-glucanase gene …
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Isolation, expression, purification and characterisation of a novel acetyl xylan esterase from streptomyces species ORS10
… achromogenes (99% identity). A small-insert genomic library was constructed and a putative acetylxylan esterase (AXEase) gene, axe10, was identified. The enzyme, Axe10, has moderate similarity to α/β hydrolase proteins, and contains an esterase/lipase superfamily conserved domain …
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Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052
… stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using this approach, a truncated scr operon was isolated. The remainder of the operon was isolated in the second and third stages by recovering a plasmid integrated …
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Partial isolation and characterization of the mouse calmodulin genes
… II pseudogene were isolated from a 129SVJ mouse genomic library. When the Cam II cDNA was used to determine the chromosomal location of the Cam II gene, the results indicated that at least two likely pseudogenes exist in addition to the functional gene. Using a probe that contained only sequence …
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Developmental genetic studies on Thiobacillus ferrooxidans
… to T. ferrooxidans could not be shown. A genomic library of T. ferrooxidans ATCC 33020 was constructed in the plasmid vector, pEcoR251, for the purpose of cloning a recA-like gene from this organism. The library consisted of approximately 1,78 X 10⁴ clones carrying chromosomal DNA …
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