Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"Genomic integrity."”.
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
PART1 1 I. INTRODUCTION 2 II. MATERIALS AND METHODS 4 A. Cell culture and treatments 4 B. Generation of RSF1 KO cells 4 C. Transfection and RNA interference 4 D. Plasmid and purification of recombinant proteins 5 E. Immunoprecipitation and in vitro binding assay 5 F. In vitro kinase assay 6 G. Cell …
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Regulation of neuronal genomic integrity through histone deacetylase cooperativity
While the mechanisms preserving genomic integrity are well defined in proliferating cells, corresponding pathways in postmitotic neurons remain poorly understood. In this report, I characterize the functions of two lysine deacetylases, SIRT1 and HDAC1, in the neuronal response to DNA double strand …
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Mechanisms for maintaining genomic integrity during chromosome segregation in budding yeast
Maintaining genomic integrity is crucial for an organism's fitness and survival. Regulation of chromosome segregation requires complex surveillance mechanisms that vary for different loci within the genome. This thesis focuses on two complexes, monopolin (made up of Lrs4, Csml and Maml) and …
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Roles of RNA interference and DNA mismatch repair in maintaining genomic integrity in Cryptococcus pathogens
<p>Microorganisms must regulate genomic stability to strike a balance between excessive deleterious mutation and evolutionary stagnation to successfully compete and endure within their ecological niches. Two important mechanisms involved in maintaining genomic stability are RNA interference (RNAi) …
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Understanding The Mechanism of Genomic Instability During Replicative Aging In Budding Yeast
… most important hallmarks of aging is increased genomic instability. However, there remains much ambiguity as to the cause. I am studying the replicative life span (RLS) of the genetically tractable model organism Saccharomyces cerevisiae, or budding yeast using the innovative “mother enrichment …
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Integration of ATM, ATR, and DNA-PKcs Signaling Maintains Genome Integrity During Neurogenesis
… when complex DNA lesions arise to maintain genomic integrity. ATM, ATR, and DNA-PKcs (encoded by PRKDC) are related phosphatidylinositol-3-kinase like serine/threonine kinases (PIKK) that collectively regulate the DDR network. Studies have demonstrated these kinases can phosphorylate many of …
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Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach
The genomic integrity of the cell is under the constant threat of DNA damage from endogenous and exogenous sources. In response to DNA damage mammalian cells elicit a highly complex, yet coordinated series of cellular responses, which are triggered by the detection of DNA lesions and ultimately …
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Analysis of cell cycle surveillance mechanisms in meiosis
… This poses a significant hazard to the genomic integrity of the cell. Studies in a number of organisms have unveiled the existence of surveillance mechanisms or checkpoints that couple DNA repair and microtubule integrity to meiotic cell cycle progression. Through their action, aberrant …
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The Role of Hiwi in Stem Cell Maintenance and Sarcomagenesis
… maintain transposon silencing, and thus ensure genomic stability. A detailed mechanism by with Piwis suppress transposon migration in the germline remains an area of active investigation, but is thought to occur via DNA methylation of transposon regions. In this way, Piwis are critical for …
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The Mitotic Exit Network detects spindle position and anaphase entry
… mitotic exit in budding yeast. The MEN protects genomic integrity by ensuring chromosome segregation is complete prior to cytokinesis. Two signals are required for MEN activation: (1) movement of the nucleus into the daughter cell and (2) anaphase onset. These two events only coincide after …
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Higher Order Chromosome Organization and Recombination Dynamics of Meiotic Prophase I In Mouse Spermatocytes
… of each pathway towards maintaining germline genomic integrity are poorly understood <em>in vivo</em>at endogenous sites, especially in a mammalian system. More importantly, how germline genome integrity is ensured at both the DNA level by recombination activity and by higher order chromosome …
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Mechanism of regulation of human RAD51 recombinase through post translational modifications & mediator proteins
… deleterious forms of DNA damage and maintain genomic integrity. RAD51 along with its bacterial counterpart RecA, bacteriophage UvsX and archaeal RadA have been subjected to genetic and biochemical scrutiny resulting in a plentitude of mechanistic and functional information on formation, …
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Inference of 3D structure of diploid chromosomes
… role for gene regulation, DNA replication, and genomic integrity. Through the development of chromosome capture experiments (such as 3C, 4C, Hi-C) it is now possible to obtain the contact frequencies of the DNA at the whole-genome level. In this thesis, we study the problem of reconstructing the …
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Correction of Cystic Fibrosis-Specific Induced Pluripotent Stem Cells
… by whole genome and exome sequencing to evaluate genomic integrity after reprogramming and correction. Furthermore, <em>in vitro </em>differentiation of corrected iPSCs towards lung and thyroid lineages produces cells that express the mature CFTR protein isoform. This is the first report of …
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EXAMINING THE ROLE OF THE XAB2 PROTEIN IN HOMOLOGOUS RECOMBINATION
… break (DSB) repair is critical to maintain genomic integrity and cell viability. DSBs can occur during the course of cell cycle during replication or transcription, or by exogenous agents such as chemicals or ionizing radiation. For my thesis, I studied homologous recombination (HR), which …
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Molecular Mechanisms of DNA Protection by Tardigrade Damage Suppressor Protein (Dsup): Structural Adaptation and Functional Interactions
… oxygen species (ROS) pose significant threats to genomic integrity, leading to DNA damage, mutations, and disease. Tardigrades, extremotolerant organisms, exhibit remarkable resilience to such stressors, largely attributed to the DNA damage suppressor protein (Dsup). This study investigates the …
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Single-molecule FRET study on the RecA-mediated DNA repair
The RecA protein helps maintain genomic integrity through recombination. Using single-molecule assays and hidden Markov modeling, we show the most direct evidence that a RecA filament grows and shrinks primarily, one monomer at a time only from the extremities. Both ends grow and shrink, contrary …
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The Role of the RNA-binding Protein ZFP36L1 in Suppression of Replication Stress-induced Genomic Instability
… and the consequent failure to preserve genomic stability. ZFP36L1, a member of the ZFP36 family of CCCH tandem zinc finger (ZF) proteins, is an RNA-binding protein that plays a crucial role in the post-transcriptional regulation of gene expression underlying physiological processes and …
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Understanding The Repair Mechanisms at Ionizing Radiation-induced Damage in The Human Genome
… factor-A (SAF-A), that is crucial to maintain genomic integrity.
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DNA Damage Response in Huntington’s Disease and Naked Mole Rat Brain Ageing
… may contribute to CAG repeat instability and genomic instability in HD. Integration of these findings highlights the central importance of genomic stability and cellular homeostasis in brain health and disease. The NMR's remarkable resistance to age- related brain pathology underscores the …
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