Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"Genomic Libraries"”.
-
Construction and phenotypic screening of mid-size insert marine microbial environmental genomic libraries
Functional screening of environmental genomic libraries permits the identification of clones expressing activities of interest without requiring prior knowledge of the genes responsible. In this study, protocols were optimized for the construction of mid-size DNA insert, inducible expression …
-
Gene cloning studies in two nocardioform bacteria
… features for the construction of actinomycete genomic libraries. Cloning of DNA into the unique Bg1II endonuclease site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived …
-
Development and characterization of DNA markers for two avian species
Central to the application of genomics to animal agriculture are DNA markers, especially microsatellites and single nucleotide polymorphisms. These markers are the resources necessary for constructing genetic maps and for determining how improved and unimproved animal breeds are related. Here, DNA …
-
Palindromes on the human X chromosome : testis-biased transcription, gene conversion and evolution
Recent genomic studies of the Y chromosome revealed massive, testis-specific palindromes that span 30% of the chromosome and are subject to gene conversion. We conducted studies to determine whether similar palindromes exist on the human X chromosome and, if they exist, to what degree they share …
-
Combining microbial functional metagenomics and Caenorhabditis elegans genetics to uncover and characterise novel bioactive compounds
… thesis was to develop a novel functional (meta) genomic screen for the identification of inhibitors that target the nematode Caenorhabditis elegans. Environmental DNA from marine habitats was expressed in Escherichia coli and the resulting functional (meta) genomic libraries were screened by …
-
Isolation and analysis of cotton genomic clones encompassing a fatty acid desaturase (FAD2) gene
… in cotton, a FAD2 gene was isolated from two genomic libraries using an Arabidopsis FAD2 hybridization probe and a cotton FAD2 5¢ -flanking region gene-specific probe, respectively. A cotton FAD2 gene was found to be in two overlapping genomic clones by physical mapping and DNA sequencing. The …
-
Development and Characterization of Microsatellite Markers for the Grain Amaranths (Amaranthus spp. L.)
… A total of 1457 clones were sequenced from three genomic libraries enriched for the microsatellite motifs AAC, AAT and AC. Of these, 353 (24%) contained unique microsatellites. An additional 29 microsatellite loci were identified among 728 BAC-end sequences of a newly developed amaranth BAC …
-
Characterization of a murine activated lymphocyte antigen (MALA-2) : the murine homologue of human intercellular adhesion molecule 1 (ICAM-1)
… MALA-2 was characterized by cDNA cloning, genomic cloning, and analysis of an assumed alternatively spliced mRNA. Two cDNA clones were isolated from an NS-1 cDNA library using oligonucleotide probes constructed from amino acid sequences of peptides derived by tryptic cleavage. The two …
-
Characterization of the cytochrome P-450IIC3 gene and 5' flanking region of P-450IIC1
… induced by phenobarbital. Two rabbit liver DNA genomic libraries were screened for the presence of the cytochrome P-450IIC3 and IIC1 genes. DNA from genomic clones C3.1, C3.2, C3.3, and C1.1 were isolated, and subcloned into phage M13 mp11 and phagemid pTZ18R for sequencing by the chain …
-
Klonering en uitdrukking van die mensgeen vir interleukin-3 (IL-3)
… attempt to isolate the IL-3 gene from cDNA and genomic libraries. For this IL-3 specific oligonucleotides were designed and synthesized to be used as probes for the screening of libraries as well as primers during PCR for the amplification of parts of the IL-3 gene. The amplification products …
-
Distribution and biosynthesis of LH-RH and structurally related peptides
… screening of mRNA from certain tissues, cDNA and genomic libraries as well as genomic DNA restriction endonuclease fragments to establish the nature of the LH-RH precursor and related molecules. Acetic acid extracts of nineteen rat tissues were assayed in serial dilution for LH-RH immunoreactivity …
-
Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
… sequences to avoid unnecessary sequencing from genomic libraries and detection of common pathogenic viruses of the respiratory tract. Four different structures of DNA linear double stranded DNA (dsDNA, 48 kb), linear single stranded DNA (ssDNA, 3.6 kb), small circular dsDNA (2.6 kb) and a large …
-
Studio molecolare dei meccanismi dell'autoincompatibilità gametofitica in pero europeo (Pyrus communis)
… until very recently; in apple, the use of genomic libraries allowed the detection of two F-box genes linked to each S haplotype, called SFBB (S-locus F-Box Brothers). In Japanese pear, three SFBB genes linked to each haplotype were cloned from pollen cDNA. The SFBB genes exhibit S …