Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Genomic DNA Library"”.
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Cloning and characterization of MET2 in Pichia pastoris
… complementation through transformation with a genomic DNA library.</p> <p>Bioinformatics show that the P. pastor is MET gene has 54% amino acid identity with 68% similarity to the S. cerevisiae MET2 gene, which codes for homoserine O-transacetylase. We have constructed expression vectors for …
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Characterization of the antifreeze peptide system of the Antarctic eel pout, Pachycara brachycephalum
… zoarcoids. Six AFP genes were isolated from a genomic DNA library. Four of the genes are similar in sequence to other zoarcoid AFP genes, encode proteins similar to the predominant AFP (AB1) and are termed ""classical"" genes. The fifth AFP gene contains a 648-bp insertion with the 3$\sp\prime$ …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… (Rutherford, et. aI., 1991). A complementary DNA (cDNA) encoding glycogen phosphorylase-}, gp-l, was isolated from a λgtll expression library made from amoebae stage mRNA. The 5' upstream region of the gp-l gene was cloned by inverted polymerase chain reaction (IPCR) and partial genomic DNA …
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Subcloning and regulation of a human intestinal sodium-phosphate cotransporter gene
… modulate the activity of this transporter. A cDNA encoding a novel human small intestinal Na⁺-P i transporter was isolated from a human intestinal cDNA library. This cDNA encodes a 689 amino acid polypeptide which is different from the renal NaPi cotransporters. This human intestinal NaPi …
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Diversity and characterization of novel Cytochrome P450 2 genes in the marine teleost Fundulus Heteroclitus
… the killifish, Fundulus heteroclitus. Multiple cDNAs from three CYP2 subfamilies were isolated from killifish cDNA libraries. Sequence analyses showed that one cloned from liver is related to trout CYP2K1. The other cDNAs were classified into two new subfamilies, CYP2N and CYP2P. CYP2N1, CYP2P2, …
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Studies on the Molecular Biology of Naegleru Fowleri and Identification of N. Fowleri in the Environment
… been sequenced, therefore, we have constructed a genomic DNA library to search for putative virulence factors or drug targets. We have performed partial sequencing of 155 plasmids and have identified putative genes for cell motility, chromosome segregation, gene regulation, protein synthesis and …
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Isolation and analysis of a corprinus cinereus DNA fragment showing homology to a fimbrial cDNA from ustilago violacea
… points ranging from 5.6 to 5.8. A fimbrial cDNA derived from U. vio/acea was used to identify DNA restriction fragments from C. cinereus and S. commune showing homology to the fimbrial transcript of U. vio/acea. Heterologous hybridization with this cDNA was used in order to screen a C. …
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The Role Of Cardiolipin In Iron Homeostasis And Glutathione Metabolism
… The suppressor screen utilizing a high copy genomic DNA library led to the identification of fifty putative suppressors of crd1Δ. Five plasmids were intially sequenced, and interestingly, each plasmid contained the same region of chromosome III, spanning four genes. One of the genes was LEU2, …
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Cloning, sequencing and functional analysis of the chicken tyrosine gene promoter
… differentiation in chick embryos, a chicken genomic DNA library was screened for tyrosinase-encoding sequences using a mouse tyrosinase cDNA probe. Two identical hybridising clones were identified. Restriction mapping and sequencing revealed that both clones contained a 4.3 kb genomic DNA …
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Cloning and characterisation of gonadotropin-releasing hormone (GnRH) receptors in the cichlid (Haplochromis burtoni) and the zebrafish (Danio rerio)
… subtype from cichlid (Haplochramis burtoni) genomic DNA. Furthermore, a near full-length cDNA, encompassing transmembrane domain 1 through to transmembrane domain 7 of the GnRH receptor, was cloned from cichlid RNA by reverse transcriptase PCR. This region of the receptor shares approximately …
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… mutations and/or overexpression of randomized DNA fragments. When DNA fragments are obtained from a common genome or metagenome and packaged into the same expression vector, this is referred to as a DNA library. Generating quality DNA libraries that incorporate broad genetic diversity is …