Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 335 for “"Genomic DNA"”.
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On-demand production of deoxynucleotides from genomic DNA
… 5'-triphosphate (dNTPs) building blocks of DNA, in a manner that can be integrated into a NAAT workflow. A unique top-down approach to produce dNTP monomers from a genomic DNA polymer is investigated, contrary to traditional bottom-up synthesis methods, which rely on inaccessible precursor …
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Mechanisms of monohalogenated acetic acid induced genomic DNA damage
… that monoHAAs were directly alkylating DNA was tested and rejected when no damage accumulated in acellular DNA. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was investigated as a possible molecular target for the monoHAAs; each of the monoHAAs inhibited GAPDH with variable efficacy. …
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A technology for detecting unselected mutational spectra in human genomic DNA
Thesis (Ph. D.)--Massachusetts Institute of Technology, Division of Bioengineering and Environmental Health, 1999.
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A computational framework for the identification, cataloging, and classification of evolutionary conserved genomic DNA
Evolutionarily conserved genomic regions (ecores) are understudied, and yet comprise a very large percentage of the Human Genome. Highly conserved human-mouse non-coding ecores, for example, are more abundant within the Human Genome than those regions, which are currently estimated to encode for …
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… mutations and/or overexpression of randomized DNA fragments. When DNA fragments are obtained from a common genome or metagenome and packaged into the same expression vector, this is referred to as a DNA library. Generating quality DNA libraries that incorporate broad genetic diversity is …
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RAD51-MEDIATED ABASIC SITES PROTECTION PREVENTS REPLICATION FORK COLLAPSE.
Genomic DNA is continuously subjected to damage caused by endogenous and environmental agents. This damage, produces as a result DNA lesions, among which abasic (AP) sites are the most prevalent in genomic DNA, occurring at an estimated rate of 10,000 to 20,000 per cell per day. Under normal …
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Methyl Chloride Transferase: Isolation, characterization,cDNA Cloning, and Expression
The genomic DNA of methyl chloride transferase of Batis maritima was also characterized and is composed of 8 exons and 7 introns.
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Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles
… SLA DQB and DRB exon 2 ($\beta\sb1$) region from genomic DNA. These oligonucleotide primers were tested on genomic DNA from ten different swine breeds (Meishan, Yorkshire, Hampshire, Duroc, Landrace, Berkshire, Chester White, Spotted Poland China, Poland China and Swedish Landrace) to confirm …
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Isolation and characterization of the tubuliform spidroin 1 promoter from the black widow spider, Latrodectus Hesperus
… widow spider, Latrodectus hesperus. Using a genomic DNA walking strategy, we have isolated an upstream segment (581 bp) of genomic DNA containing the promoter as well as the first exon of the TuSp1 gene. This upstream regulatory element was able to initiate transcription in insect cells when …
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The Mutation Analysis of GnRH1 and GnRH Receptor Gene in Korean Girls woth Central Precocious Puberty
… vi 약어 vii I. 서론 1 II. 대상 및 방법 5 A. 대상 환자군의 선별과 Genomic DNA 준비 5 1. 대상 환자군과 대조군 5 2. 성선자극호르몬방출호르몬 자극 검사 5 3. 환자군과 대조군으로부터 채혈 5 4. Genomic DNA 추출 6 B. GnRH1 와 GnRH-R 유전자 돌연변이 분석 6 1. GnRH1 유전자의 돌연변이 분석 6 2. GnRH-R 유전자의 돌연변이 분석 7 C. GnRH1 와 GnRH-R 유전자의 단일염기다형성 유전자형 분석 7 1. GnRH1 유전자 돌연변이 분석을 통한 …
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Morphological and Biochemical Analysis of Variation in Diploid Glycine Tomentella Hayata (2N = 38, 40)
… macromolecules---total protein, isozymes, and DNA. Measurements or observations regarding 54 morphological traits were recorded. Twenty-three were deemed sufficiently reliable for statistical use and were analyzed by Principal Components Analysis followed by clustering. Terminal leaflet shape …
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Control of uteroglobin gene expression
… was an activity capable of suppressing the DNA binding activity. In this work, the repressor activity was purified from nuclear extracts. The cause of this activity was found to be fragments of genomic DNA. The promoter binding activity (UGPB) was then further characterised. UGPB-UG200 …
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한국인 전신성 홍반성 루푸스에서 Fcγ 수용체 유전자들의 다형성
… 5 A. 대상 환자군의 선별과 genomic DNA 준비 ·································· 5 1. 루푸스 환자군과 대조군 ····················································· 5 2. 환자군과 대조군으로부터 채혈 ············································· 5 3. Genomic DNA 추출 …
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The identification and characterization of genomic bacterial DNA utilizing vibrational spectroscopy
… were used to study dried films of pure intact genomic Deoxyribonucleic Acids (DNA) from two species of Bacillus bacteria complexed with Tris(hydroxmethyl)aminomethane (Tris) and Ethylendiaminetetraacetic Acid (EDTA)...a new methodology for the accurate differentiation and identification of …
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Development of Novel Collection and Biosensor Detection System for Microbial Organisms
… sonication to lyse the organisms and shear the genomic DNA in preparation for detection by a DNA biosensor. Initially, the specificity of the DNA probes was determined using polymerase chain reaction and gel electrophoresis. Probes shown to be selective for a particular bacteria or fungi were …
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Organization of nifH and nifD genes in Nostoc commune UTEX 584
… (Rice et al., 1980). Southern transfers of genomic DNA prepared from N- commune UTEX 584 were digested with Hind III and hybridized with nifH-specific and nifD-specific probes. Multiple copies of nifH (three) and nifD (two) were detected. Using colony hybridization, a recombinant N- commune …
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Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment
… evaluate and optimize the sensitivity of HIV-1 DNA quantitation in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative …
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Use of RAPD markers in plant varietal development
DNA based molecular markers have played an important role in plant varietal development. With the discovery of polymerase chain reaction (PCR), very low or single copy sequences can act as molecular markers because during the PCR, DNA is multiplied to such an extent that it can be easily visualized …
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