Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 45 for “"Genome-wide analysis"”.
-
Genome-wide analysis of transcription factors and chromatin in S. cerevisiae
… play in regulating the expression of the yeast genome was not known. Genome-wide expression analysis of mutations in the Rpbl subunit of RNA polymerase II, the Srb4 subunit of Srb/Mediator complex, and the Kin28 subunit of TFIIH demonstrated that these factors are required for the transcription …
-
Genome-Wide Analysis of Transcription Factors Ascl1 and Ptf1a in Development and Cancer
… direct transcriptional targets were identified genome-wide in the multiple tissues using ChIP-Seq and RNA-Seq. Overwhelmingly, Ascl1 and Ptf1a directly regulate different gene programs important for each tissue. Within a given tissue, the specificity of Ascl1 and Ptf1a function is partly …
-
The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
… replication origin specification and activation genome-wide, by manipulating the in vitro replication reactions. Y RNA removal resulted in fewer ds-iniSeq origins with lower activities genome-wide. xNuRD restored the number and activities of origins genome-wide to similar levels when in the …
-
Genome-wide analysis of transcriptional expression programs, regulatory networks and Cis-regulatory sequences in Saccharomyces cerevisiae
… of gene expression at the level of the entire genome, allowing researchers access to rich datasets and promising new levels of depth in the understanding of transcriptional regulation. Our lab has made use of these technologies both to measure the levels of all mRNA transcripts within a …
-
A microfluidic platform for the genome-wide analysis of electrical phenotype : physical theories and biological applications
… The objective of this work is to obtain a genome-wide mapping of genotype to electrical phenotype in the budding yeast Saccharomyces cerevisiae. Towards this end, we present: (1) the development of a novel equilibrium gradient separation method, called isodielectric separation (IDS); (2) …
-
Integration of Nuclear- and Extranuclear-Initiated Estrogen Receptor Signaling in Breast Cancer Cells
… tumors. In this thesis, we have used multiple genome-wide analysis tools to elucidate various ways that are utilized by extranuclear-initiated estrogen receptor pathways to impact gene expression in MCF-7 breast cancer cells.
-
Prediction of Dna Methylation Based On Genomic Architecture and Applications of Positional Weight Matrices
… which insert themselves randomly into the genome causing possible loss of gene function. In our genome wide analysis of human genes, results suggested that 22% of the genes in cancer were predicted to be methylation-prone; in cancer these genes are generally down-regulated and function in …
-
The Novel Regulation of Histone Modification In Cancer Development
… (HAT) GCN5 for subsequent H3 acetylation. Genome-wide analysis of chromatin immunoprecipitation followed by sequencing (ChIP-seq) data sets reveals that NEDD4 regulates glucose-induced H3K9 acetylation at transcription starting site (TSS) and enhancer regions. Integrative analysis of …
-
The evolution and specificity of RNA splicing
… are not encoded in contiguous segments in the genome but are rather punctuated by long interruptions known as introns. These introns are copied from generation to generation, and even from cell to cell as a person grows from an embryo into an adult. Each time a gene is activated, the cell must …
-
Molecular characterization and functional analysis of cytochrome P450 genes in the yellow fever mosquito Aedes aegypti (Diptera: Culicidae)
… is very limited. This research was to perform a genome-wide analysis of P450 genes and evaluate the role of selected P450 genes in detoxification of three commonly used pyrethroid insecticides in the yellow fever mosquito (Aedes aegypti). Our genome-wide analysis of revealed 159 P450 genes that …
-
Spliceosomal small nuclear ribonucleoprotein particle trafficking and maturation in the nuclear compartment
… realm of the cell nucleus. Second, it allows a genome-wide analysis of snRNP recruitment to nascent transcripts, and, hence, the conclusions drawn from these studies do not depend on the sequence of any particular promoter or pre-mRNA. Indeed, it is with this assay that we have stumbled upon a …
-
Characterizing variation at short tandem repeats and their role in human genome regulation
… motifs of 1-6bp that span over 1% of the human genome. The level of STR variation and its effect on phenotypes remains mostly uncharted, mainly due to the difficulty in accurately genotyping STRs on a large scale. To overcome bioinformatic challenges in STR genotyping, we developed lobSTR, an …
-
Monoallelic Gene Expression In Human Neural Stem Cells
… More recently, studies have reported evidence of widespread autosomal RME in human lymphoblastoid cells (Gimelbrant et al. 2007) and mouse neural stem cells (Wang et al.2010; Li et al. 2012). However, the extent to which RME occurs in human neural tissue is unknown. Using a genome-wide analysis of …
-
Estimating evolutionary parameters and detecting signals of natural selection from genetic data
… in DNA profiling technologies have enabled genome-wide analysis of thousands of individuals from a diverse array of human populations. These new analyses can answer fundamental questions about human population differences, natural selection, and admixture. However, with this deluge of newly …
-
Role of the NF-kappaB-HIF Crosstalk in Inflammation and Hypoxia
… to more angiogenesis in an in vitro model.<br/>A genome-wide analysis was also performed in order to understand the role of HIF in inflammation. This has revealed 2495 potential novel HIF-dependent target genes in inflammation, in which several of them were known hypoxia-induced HIF, or NF-kappaB …
-
The evolution and genetics of virus resistance in Drosophila
… pipeline, I recovered partial viral genomes which were used to reconstructed their phylogeny. Then, I experimentally explored the impact of the newly isolated viruses on Drosophila infected with Wolbachia, a mutualistic endosymbiotic bacterium that protects the flies against RNA …
-
Design, synthesis, and analysis of conjugated activators for modulation of alternative splicing
Recent genome wide analysis has estimated that >95% of human genes are alternatively spliced, allowing for the production of many protein isoforms from a single gene. Splicing is controlled by SR proteins, factors which bind to exonic splicing enhancers (ESEs) through RNA binding domains and …
-
Interrogation of CRISPR-Cas targeting specificity for mammalian genome engineering
… DNA endonucleases enable high efficiency genome editing across the biological diversity for research, industrial, and biomedical applications. Human genome editing with CRISPR-Cas just recently made its debut in human clinical trials and has immense therapeutic potential to fix …
-
Understanding the molecular mechanism of postharvest seed coat darkening in pinto beans
… transcript abundance, metabolite analysis, and inheritance study, I found a novel allele of P, Psd, whose partial loss-of-function slows down seed coat darkening by reducing the PA levels. Compared to P, Psd contains an additional glutamate residue and an arginine to histidine …
-
Genome-Wide Localization And Novel Deposition Pathways Of Histone Variant H3.3 In Embryonic Stem And Neuronal Precursor Cells
<p>The eukaryotic genome is composed of chromatin, a complex polymer of genomic DNA, RNA, and closely associated proteins. Histone proteins form the core of the nucleosome, the fundamental repeating unit of chromatin. Variant histone proteins play critical roles in the epigenetic regulation of gene …
Page 1 of 3