Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Genome mining"”.
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Genome Mining for Actinomycete Biosynthetic Gene Clusters
Whole-genome sequencing has shown that the large (8-12 Mbp) genomes of Streptomyces and allied genera of Gram-positive filamentous bacteria house a rich and previously underestimated repertoire of gene clusters for biosynthesis of specialised metabolites, including antibiotics, immunosuppressants …
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Genome mining for natural product biosynthesis: Amino acids, nonribosomal peptides, and ribosomal peptides with posttranslational modifications
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Strategies for thiazole/oxazole-modified microcin discovery
… compounds. With the advent of inexpensive genome sequencing it has become apparent that bacteria produce a larger array of natural products than was previously believed. This new wealth in sequence data has potential to be helpful for the discovery of novel compounds by using genome mining. …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… natural products. The vast increase in sequenced genomes has allowed natural product discovery to move from a blind screening approach to a bioinformatics guided approach. Screening of extracts for bioactivity is not only laborious, but also leads to the rediscovery of known compounds. With a …
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Characterization of microbial primary and secondary metabolism in the marine realm
… these analyses. In my second chapter, I applied genome mining tools to analyze the gene content and expression of biosynthetic gene clusters (BGCs). The analysis of BGCs through largescale genome mining efforts has identified diverse natural products with potential applications in medicine and …
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Characterization of Microbial Primary and Secondary Metabolism in the Marine Realm
… these analyses. In my second chapter, I applied genome mining tools to analyze the gene content and expression of biosynthetic gene clusters (BGCs). The analysis of BGCs through largescale genome mining efforts has identified diverse natural products with potential applications in medicine and …
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Knowledge discovery in biological databases : a neural network approach
… discovery, in databases, also known as data mining, is aimed to find significant information from a set of data. The knowledge to be mined from the dataset may refer to patterns, association rules, classification and clustering rules, and so forth. In this dissertation, we present a neural …
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Genomic analysis of mouse tumorigenesis
The availability of the human and mouse genome sequences has spurred a growing interest in analyzing mouse models of human cancer using genomic techniques. Comparative genomic studies on mouse and human tumors can be valuable in two major ways: in validating mouse models and in identifying genes …
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Exploration of the biosynthesis of lanthipeptides
… screening campaigns. With the advances in genome sequencing and bioinformatics, a variety of lanthipeptides have been identified by genome mining, presumably with more unexplored functions and molecular targets. Prochlorosins are an unusual group of lanthipeptides, which were discovered …
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Identificación y caracterización de agrupaciones de genes de biosíntesis para péptidos no ribosomales en Streptomyces sp. CS113
… que ha llevado a utilizar la minería genómica (genome mining) como estrategia para identificar agrupaciones de genes (BGCs) que codifiquen nuevos compuestos. La utilización de esta estrategia requiere poder identificar BGC desconocidos (BGCs crípticos) y que se expresen, ya que en muchos casos …
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Identification and Characterization of Antibiotic Biosynthetic Gene Clusters in Pantoea
… distribution across bacterial species, and genome mining tools were unable to identify the cluster, suggesting the metabolite may be novel. Chapter 3 describes the characterization of the two predicted MFS transporters, Pnp3a and Pnp3c, using genetic, phylogenetic, and bioinformatic …
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Heavy metal inducible antimicrobial activity of streptomyces spp. isolated from the Leadhills and Wanlockhead lead mines in Scotland, Uk
… rise of multidrug resistant infections. Whole genome sequencing and genome mining have revealed that Streptomyces spp. possess a range of biosynthetic gene clusters for secondary metabolites, however many of them are silent and cryptic under conventional laboratory conditions. It has also been …
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On the biosynthesis and discovery of ribosomally synthesized and post-translationally modified peptides
… regarding substrate processing remained. Genome mining efforts revealed an uncharacterized TOMM gene cluster in Bacillus sp. Al Hakam (Balh) that contained substrates and enzymes that ultimately overcame previous limitations. Initial reconstitution of the enzymes revealed cyclization of …
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Enzymology of Gentamicin Biosynthesis
… is 6'-N-methylation of gentamicins C1a and C2. Genome mining and cell-free assays were used by the group of Professor Yuhui Sun (Wuhan University) to identify genL, a methyltransferase gene encoded elsewhere on the M. echinospora genome and capable of this catalysis. Here, in vitro reactions …
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Analysis and Engineering of Polyene Biosynthesis in Actinomycetes
… reasons for this are discussed. In Chapter 5, genome mining was used to explore the rare genus Saccharopolyspora which has previously provided the valuable erythromycin A and spinosyn. The genomes of 20 strains of Saccharopolyspora were analysed, revealing over 400 gene clusters, including …
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Molecular genetic investigation of autosomal dominant hemifacial microsomia
… genes were identified from the Draft Human Genome through genome mining and bioinformatics tools. This essentially involved cross-tabulating the genes and predicting genes I the two known hemifacial microsomia candidate regions and by comparison of gene names, aliases, acronyms, functions, …
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Characterising Antarctic marine invertebrate microbiomes and their antimicrobial potential
… for their antimicrobial activity, with whole genomes sequenced from the two most promising candidates. Finally, chemical extracts of two species of Antarctic marine invertebrates were examined for antimicrobial potential, on account of their potential to harbour novel chemical defences. This …
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Mechanistic studies of lanthipeptide biosynthesis
… process described in this thesis may assist the genome mining and synthetic biology efforts towards novel lanthipeptides for therapeutic purposes and other applications.
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Genomics approaches to exploit the biotechnological potential of marine sponge-derived Streptomyces spp. isolates
… state-of-the-art methodologies related to genome mining and bioinformatics-based pipelines, together with molecular and synthetic biology, were employed and proved to be extremely useful in helping to uncover the biotechnological potential of marine sponge-derived Streptomyces isolates. …
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