Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Genome Synthesis"”.
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Engineering Escherichia coli as a general genome synthesis platform
Writing DNA at genome scale provides unprecedented control in engineering biological systems. This includes the ability to reprogram the genetic code to expand biological functionality and equips biologists with tools to ask previously unanswerable questions on genome organisation and regulation. …
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Engineering genetic isolation in Escherichia coli and developing synthetic genomics tools to efficiently assemble megabase-scale genomes
Chapter 1: Introduction DNA synthesis plays a central role in our ability to manipulate biological systems at various scales. Short synthetic DNA oligos used as primers for polymerase chain reaction (PCR) enable the specific amplification of chosen DNA sequence and synthetic genes are critical for …
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Fabrication and control of microfluidic devices for on-chip synthesis
… compounds. The sequencing of the Human Genome has been completed, as well as that of many other organisms. Current work is now shifting towards the production of biological macromolecules. More specifically, this includes gene and genome synthesis from user-defined sequences. A series of …
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Rewriting the genome of Escherichia coli
… scale is opening the door to writing entire genomes; this constitutes a powerful approach to address fundamental biological questions, and may enable the creation of designer organisms with useful properties. One interesting avenue for investigation is the creation of recoded genomes, where …
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Comparative study of the kinetics and mechanisms of adenovirus and bacteriophage PR772 inactivation by disinfectants used for global safe drinking water
… could still attach to host cell monolayers, but genome synthesis as well as late hexon mRNA and early E1A mRNA transcription were inhibited. When exposed to free chlorine, PR772 had remarkably similar inactivation kinetics compared to HAdV-2. Like HAdV-2, free chlorine inactivated PR772 could …
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Synthesis, Characterisation and Evolution of Escherichia coli with a Compressed Genetic Code
The design and synthesis of entire genomes is a powerful approach that allows the study of biological functions and the creation of new ones: Study-by-synthesis promises a better understanding of the genomic architecture and regulatory mechanisms. Furthermore, synthetic genomes may enable the …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
… systems that permit replication of the viral RNA genome and virus particle production. However, the exact functions of the viral proteins, particularly those engaged in RNA synthesis, are poorly understood. It is thought that NS4B, one of the replicase components, induces the formation of …