Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Genipin"”.
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Genipin-crosslinked electrospun collagen nanofibers
… In this thesis, a novel approach, using genipin (a natural crosslinking agent), was introduced to crosslink the electrospun collagen nanofibers. Genipin has been proven to be significantly less cytotoxic compared to glutaraldehyde.
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Identification of genipin as a potential treatment for type 2 diabetes
… aimed to examine whether natural compound genipin promotes intestinal GLP-1 secretion and exerts anti-diabetic effects. I found that genipin rapidly increased GLP-1 secretion from intestinal L-cells, with 10 and 100 μM concentration inducing significant incretin hormone release. L-cells …
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Biomaterials-tissue interaction of an injectable collagen-genipin gel in a rodent hemi-resection model of spinal cord injury
… thesis was to develop an injectable collagen-genipin (Col-Gen) hydrogel to facilitate the intrinsic regenerative response after SCI by promoting the population of the defect with astrocytes through a provisional scaffold pennissive of astrocyte migration. The specific aims of the thesis …
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Collagen scaffolds and injectable biopolymer gels for cardiac tissue engineering
… scaffold. Soluble type I collagen gels with genipin and transglutaminase cross-linkers, and gelatin-hydroxyphenylpropionic acid (Gtn-HPA) gels, the cross-linking of which are modulated by horse radish peroxidase and hydrogen peroxide, were investigated. The gels were characterized on the …
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Gradients in Collagen Films: Effects of Mechanical and Chemical Properties on Directional Cell Response
… chemical properties, while crosslinking using genipin altered mechanical behaviour without changing surface chemistry. By adjusting the rate of immersion and solution concentration, gradient shape could be controlled. The effect of crosslinking chemistry was evaluated on uniformly treated …
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A comparative study of common crosslinking agents for electrospun collagen scaffolds
… crosslinking methods using glutaraldehyde, genipin, or Dimethyl aminopropyl)-N'-ethyl carbodiimide with and without N-hydroxysulfosuccinimide. The fibers were characterized using both chemical and physical tests to compare the effectiveness of different crosslinker and crosslinking …
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Multiscale Characterisation of Ice and Collagen: Physicochemical Development of Lyophilised Scaffolds for Tissue Engineering
… (HDFs) to films crosslinked with EDC-NHS, genipin and tissue transglutaminase 2 (TG2) revealed that genipin- and TG2-treated samples did not lose their integrin specificity unlike EDC-NHS crosslinked samples. Although both EDC-NHS- and genipin-treated films possessed a high tensile modulus, …
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The effects of biophysical and biochemical cues on tendon cells: a study of underlying mechanisms in tendinopathy
… oxygen species (ROS) production characterised. Genipin treatment was hypothesised to restore biomechanical properties in collagenase-induced tendinopathy in rat Achilles tendons. Healing outcomes were characterised using biomechanical and histological measurements. hTDCs were cultured on plastic …
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Determining Mechanisms by which Pathological Extracellular Matrix induces Ocular Hypertensive Phenotypes in Human Trabecular Meshwork Cells
… inhibitor (TGFβRi). (3) To determine whether genipin-induced crosslinked CDM (XCDM) stiffens hTM cells via dysregulating β-catenin and Yes-associated protein (YAP)/Transcriptional co-activator with a PDZ-binding motif (TAZ) signaling pathways, hTM cells were seeded on CDMs/XCDMs for 24h. Where …
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Fabrication and evaluation of a collagen-based fiber-gel three-dimensional construct for peripheral nerve repair
… the fiber component, chemical cross-linking with genipin and glutaraldehyde are evaluated. The result is a construct exhibiting mechanical integrity for facilitating adult Schwann cell orientation and the guidance and survival of adult dorsal root ganglion neurons in a co-culture 3-D system.
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TISSUE ENGINEERING CELLULARIZED SILK-BASED LIGAMENT ANALOGUES
… glutaraldehyde, carbodiimide hydrochloride, and genipin, was also investigated as a means to both improve the mechanical stability and slow the rate of degradation of the structures. Fiber orientation and scaffold anisotropy were controlled through varying fabrication parameters, and proved …
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Microfabrication Approaches for Understanding the Role of Vascular Mechanics in Progressive Diseases
… tissue scale. We modify substrate surfaces with genipin, a natural crosslinker, to extend culture times of in vitro vascular tissues that mimic native tissue structure and function. We successfully validate our technique, showing maintenance of patterned structural alignment and mechanical …
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Investigation into the development of a gelatin-based biocomposite for wound-management
… diisocyante, poly ether epoxide, and genipin) was carried out. The scaffolds, although showing a uniform porosity, had the tensile strength (240 kPa) lower than the reported value for natural skin (850 kPa). To strengthen the porous scaffold, a middle layer was applied and bonded to …
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A NEW STRATEGY FOR MOSQUITO BIOCONTROL BASED ON THE IMPROVEMENT OF BACILLUS THURINGIENSIS EFFECTIVENESS
… molecules of natural origin, namely chitosan and genipin. This product has been designed to include a Bti-based formulation to induce larval toxicity, Saccharomyces cerevisiae cells to induce phagostimulation and air bubbles to allow its flotation in water. Our results have shown the efficacy of …
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Production of Compositional Analogues of Bone Based on Electrophoretically Deposited Mineralised Collagen Films
… an assessment of glutaraldehyde, EDC-NHS, and genipin crosslinkers on collagen films produced using EPD was undertaken. The data revealed no significant differences in the morphology and proliferation of human osteoblasts between crosslinking conditions. However, the alkaline phosphatase …
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SMART POLYMERIC DRUG NANOCARRIERS FOR BIOMEDICAL APPLICATIONS
… confer high biomimetic ability to the material. Genipin (GP) was selected as cross-linker for chitosan, due to its natural origin and biocompatibility. The cement composition was optimized to obtain the desired hardening time and dissolution rate and then reinforced with collagen. Moreover, …