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Showing 1 to 18 of 18 for “"Genetically Modified Mice"”.
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Measuring material properties of tectorial membranes from normal and genetically modified mice
With the discovery of hearing disorders caused by mutations in proteins expressed in the tectorial membrane (TM), the importance of the TM in cochlear mechanics has never been clearer. However, the exact role of the TM in cochlear mechanics remains a mystery. In this thesis, I have investigated …
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IMPACT OF CHOLINE SUPPLEMENTATION ON GENETICALLY MODIFIED MICE: NEW INSIGHTS IN THE MODULATION OF CARDIOVASCULAR RISK
Le malattie cardiovascolari su base aterosclerotica sono ancora oggi una delle principali cause di morte nel mondo. Negli ultimi anni, evidenze scientifiche suggeriscono un ruolo del microbiota intestinale nella modulazione del rischio cardiovascolare e grande attenzione è stata posta sulla …
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Generation of genetically modified mice with conditional alleles of Dyrk1a : a minimal animal model of Down syndrome
Down Syndrom (Trisomie 21) ist die Hauptursache für geistige Behinderung. Die “Down Syndrom kritische Region“ auf dem menschlichen Chromosom 21 ist ausschlaggebend für die Trisomie 21-assoziierte geistige Behinderung und für die Monosomie 21-assoziierte Mikrocephalie. Unter den bisher ungefähr zwei …
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Development of a mouse model for the study of osteoarthritis pain
… pain behaviours in relevant OA models in mice. Several models including spontaneous, surgical and chemically induced OA were explored before selecting partial medial meniscectomy of C57BI/6 mice for in-depth study. The development of degenerative joint disease was confirmed using histology …
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Wireless Telemetry System for Implantable Sensors
… on small laboratory animals, such as genetically modified mice. These subjects are used to help researchers develop medication and cures for humans. To understand the effects of the treatments, innovative telemetry systems are developed, that enable remote real-time cardiac monitoring. …
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Absence of Enhancer of Zeste Homolog 2 Promotes the Progression of KRAS-Driven Pancreatic Ductal Adenocarcinoma
… response to injury. To address this hypothesis, genetically modified mice with targeted deletion of the SET domain of Ezh2 +/- KRASG12D were exposed to cerulein-induced pancreatic injury and examined for pancreatic lesions. Histological analysis for markers of tissue damage and inflammation …
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Understanding the role of cholinergic tone in the pedunculopontine tegmental nucleus
Genetically modified mice targeting the vesicular acetylcholine transporter (VAChT) gene have been used as a tool to better understand the role of ACh signaling in specific regions of the brain (Prado et al., 2006; Guzman et al., 2011; de Castro et al., 2009). VAChT stores acetylcholine (ACh) in …
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Influx and Efflux of Adenine Nucleotides From Erythrocytes Regulate Oxygen Transport and Systemic Metabolism
… oxygen binding ability. Here, using a series of genetically modified mice, we investigated a previously unrecognized pathway of adenine nucleotide influx and efflux that modulates erythrocyte’s oxygen binding ability and controls systemic metabolism. Our studies show that the loss of AMPD3 and …
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Investigating the phenotype and function of tissue-resident B cells in mouse and human non-lymphoid organs
… with an expanded population evident in pet-store mice. Extravascular B cells had less diverse Igh repertoire with fewer N-additions compared to blood, suggesting their prenatal origin. Seeding of these B cells into NLOs was independent of their antigen specificity. Using strains of genetically …
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The functional role of the mammalian tectorial membrane in the cochlear mechanics
… in cochlear function, we investigated waves in genetically modified mice lacking beta-tectorin, a glycoprotein found exclusively in the TM. Tectb-/- mutant mice were previously shown to exhibit significant loss of cochlear sensitivity at low frequencies and sharpened frequency tuning compared to …
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Ethosuximide stabilizes sleep-related oscillations and reduces sleep fragmentation during alcohol withdrawal
… ethanol (CIE) vapor administration method in mice to study the effects of ethanol exposure and withdrawal on sleep and oscillations in neuronal activity between the thalamus and cortex (or thalamocortical oscillations). Sleep-related oscillations (SROs) in neuronal activity are a subset of …
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Bloodmeal determinants of Zika virus infection of Aedes (Stegomyia) aegypti mosquitoes
… addition of TGF-β) as well as via utilization of genetically modified mice (LEPRdb/db; displaying a type II diabetic/metabolic syndrome-like phenotype). Corresponding to previous reports, I found that viremic animals largely proved more infectious than artificial bloodmeals, although in some …
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Cellular regulation of platelet glycoprotein VI : in vivo and in vitro studies in mice
… previous studies it was shown that injection of mice with the anti-GPVI mAb, JAQ1, induces GPVI down-regulation, which is associated with a strong, but transient, thrombocytopenia. Using new anti-GPVI mAbs, which bind different epitopes on the receptor, it is shown in this study that GPVI …
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Molecular alterations of AMPA receptors and their effects on hippocampus dependent tasks
… to spatial reference memory, in a variety of genetically modified mice. Studies using GluR-A knock-out (GluR-A-/-) mice showed that GluR-A containing AMPA receptors are required for working memory, strengthened by those findings that expression of a transgenic GluR-A subunit in the forebrain …
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The role of extracellular signal-regulated kinases (ERKs) in T lymphocytes
… (RSKs).In the last part of this study I used genetically modified mice to evaluate whether the effects caused by inhibition of MEK1/2 in T cells are mediated by RSKs. T cells express RSK1 and RSK2. The data show no discernible impact of deleting RSK1 on T cell phenotypes. TCR activated RSK2 in …
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A dissection of class I phosphoinositide 3-kinase signalling in mouse embryonic fibroblasts and prostate organoids
… and PI(3,4,5)P3-3-phosphatase PTEN, or modified to a distinct PI(3,4)P2 signal, by SHIP-family 5-phosphatases. The PI(3,4)P2 is removed by INPP4-family 4-phosphatases. Published work has shown that PI(3,4,5)P3 signalling can also occur in endosomes and nuclei, however, there is very …
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The role of progesterone receptor in hypothalamic kisspeptin neurons
… used in biomedical and toxicologic studies in mice for the same purpose. Yet, where the rat estrous cycle remains consistently regular from one cycle to the other, the mouse estrous cycle is often irregular. Hence, vaginal exfoliative cytology may not be as good a predictor of the mouse estrous …
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Expanding the genome editing toolbox for biomedical applications
… methods has revolutionized genome editing in mice. One such strategy is CRISPR-READI, which combines adeno-associated virus (AAV)-mediated gene delivery with electroporation of Cas9-RNPs. The AAV delivers transgenes (up to 4.7 kb) as ssDNA to the zygotes, which is followed by electroporation …