Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 49 for “"Genetic transformation"”.
-
Prochlorococcus genetic transformation and genomics of nitrogen metabolism
… strains responded on a physiological and genetic level to changes in ambient nitrogen. We characterized the N-starvation response of Prochlorococcus MED4 and MIT9313 by quantifying changes in global mRNA expression, chlorophyll fluorescence, and Fv/Fm along a time-series of increasing N …
-
Regeneration and genetic transformation of rose and evergreen azalea
The overall goals of this research are to establish tissue culture systems for rose (Rosa hybrida L. and R. chinensis minima) and evergreen azalea (Rhododendron spp.), develop efficient and highly reliable regeneration systems via organogenesis and/or somatic embryogenesis, and develop gene …
-
A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
Bacterial cell genetic engineering is fundamental for research aiming to learn more about bacterial species for a broad range of applications. One method of intracellular delivery of foreign DNA during the genetic engineering process is the use of electroporation to create pores along the bacterial …
-
Development of genetic transformation system for bacteria of the genus Geobacillus /
… a reliable and convenient methodology for their genetic engineering. Therefore, the goal of this work was to create a new plasmids vector and to elaborate effective method for its transfer to the cells of geobacilli. First, two new plasmids isolated from wild Geobacillus spp. strains were …
-
Development of genetic transformation systems in creeping bentgrass (Agrostis palustris Huds.)
… bentgrass (Agrostis palustris Huds.) via genetic engineering, this study was conducted to develop genetic transformation systems in creeping bentgrass. Establishment of embryogenic cell cultures is a prerequisite for crop improvement via genetic engineering. A protocol for initiating and …
-
Development and application of novel genetic transformation technologies in maize (Zea mays L.)
Plant genetic engineering approaches are of pivotal importance to both basic and applied research. However, rapid commercialization of genetically engineered crops, especially maize, raises several ecological and environmental concerns largely related to transgene flow via pollination. In most …
-
Genetic transformation of Ceratotheca triloba for the production of anthraquinones from hairy root cultures
Submitted in complete fulfilment for the Degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2012.
-
In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… plasmid pCAMBIA 1305.1, was used to develop a genetic transformation system for S. drummondii. This plasmid contains a GUS (P-glucuronidase) reporter gene and a gene conferring resistance to the antibiotic hygromycin. The genes are placed under the control of the 35S CaMV (cauliflower mosaic …
-
Development of a genetic transformation system in the family Ceratocystidaceae with specific reference to Ceratocystis albifundus
The usefulness of transformation systems in fungal pathogens has been well described. Although functional studies in the Ceratocystidaceae is lagging behind model fungi from Aspergillus and Neurospora, the economic impact of many species in this family on both the agricultural and forestry …
-
Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
As a warm season grass native to the North American continent, switchgrass is considered as one of the most promising biofuel crops in the USA. It is a C4 plant that makes it energy efficient. Switchgrass has a deep root system that allows it to grow on marginal land with low water and nutrient …
-
Developing a Genetic Transformation Protocol for American Grapes (Vitis X Lambruscana) and Transforming Vitis to Produce Parthenocarpic Fruits
… In the second experiment, an efficient transformation method for 'Fredonia' and 'Melody Seedling' grapes, based on an Agrobacterium tumefaciens vector system, was developed. Finally, in the third experiment, 'Melody Seedling' was successfully transformed with the Agl5-iaaM gene. This is …
-
Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
Plant genetic transformation is a pivotal tool for plant biology and crop improvement. While stable genetic transformation methods have been established, their applicability remains constrained to specific plant species and genotypes. The process is further characterized by labor-intensive …
-
The contribution of competence system to the virulence of Streptococcus pneumoniae
… Some late competence genes encode effectors for genetic transformation. In recent years, the competence regulon of S. pneumoniae has been shown to cross regulate virulence. For example, Lau et al reported that the loss of function in ComB, an accessory protein to the ComA ABC transporter required …
-
Facultative sex in bacteria: Origin, timing and function
… the timing of a sexual process, natural genetic transformation, in the bacterium Bacillus subtilis, the relationships between sex, dispersal and dormancy in a variety of organisms, and the evolutionary history of natural genetic transformation, in all living things. My investigation of B. …
-
Expression of Gus Gene in Tobacco (Nicotiana tabacum) and Corn (Zea mays) Using Dimboa-Resistant Agrobacterium Strains
… microbial infection. Agrobacteriummediated genetic transformation technique can be employed to genetically alter most dicotyledonous plant species. However, such transformation has been limited in monocotyledonous plants, especially corn. One of the factors responsible for this recalcitrance …
Page 1 of 3