Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 243 for “"Genetic engineering."”.
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Genetic Engineering of Functional Large Amyloid Fibers
"Template" and "adder" proteins can be genetically encoded to produce large amyloid fibers when mixed together. Escherichia coli is used to clone a "template" protein, Gd20, which will cooperatively self-assemble with two "adder" proteins, P7Q and P7S, to yield two different large amyloid fibers. …
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Creating Genetic Engineering Tools for Investigating Bacillus anthracis.
… promoter region of <em>dnaB</em> to study the genetics of <em>B. anthracis</em>. An electro-transformation system was formulated to knock-out the -/- <em>B. anthracis</em> <em>dnaB</em> gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of <em>B. anthracis</em>. …
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Genetic engineering oleaginous yeasts for improved lipid production
… and can grow on glucose and xylose. However genetic tool for this host is very limited. We successfully transformed R. toruloides using Agrobacterium tumefaciens mediated transformation. We engineered two R. toruloides strains for increased lipid production by over-expressing the native …
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Genetic Engineering of 2,4-D Resistance in Wine Grapes
… where it is used. Since grapes have no known genetic resistance to this herbicide, breeding to incorporate resistance in commercial cultivars cannot be done. Thus genetic engineering has to be employed to produce adaptable grape cultivars. The overall objective of this study was to incorporate …
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Machine learning to promote transparent provenance of genetic engineering
… telltale signatures characteristic to different genetic designers, termed genetic engineering attribution, would deter misuse, yet is still considered unsolved. In this work, we present analysis of the biosecurity implications of improved tools for attribution, arguing that the technology has …
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Genetic engineering of sorghum and switchgrass for improved biofuel production
… on the switchgrass biomass increase through genetic engineering approach. Our results suggest that the employment of ectopic ZmGA20ox, or selection for natural variants with high level expression of endogenous GA20ox are appropriate approaches to increase biomass production of switchgrass and …
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Genetic engineering for pest resistance in potato (Solanum tuberosum L.)
Insect pests of potato cause significant losses of yields under field and storage conditions. Conventional breeding techniques for targeting genes of interest is usually laborious and time-consuming. An alternative way for targeting genes of interest into the plant genome consists of …
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Genetic Engineering as a Tool to Investigate Mitochondrial Gene Expression
… Given the recent advancements in genome engineering in mitochondria, in particular the development of base editing, it is now possible to investigate these processes in more detail than ever before. Using these reverse genetics approaches, this work aims to utilise this new toolkit to …
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Genetic engineering of bacteriophage and its applications for biomimetic materials
Filamentous bacteriophage (M13) are excellent biological build block due to their multiple peptide display system including type 8 (complete peptide display at pVIII) and type 83 (complete peptide display at both pVIII and pIII) display systems. Unlike the phagemid systems, the advantage of these …
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Genetic Engineering of Plant Seeds to Increase Thiamin (Vitamin B1) Content
Thiamine (Vitamin B1) in the form of thiamine pyrophosphate (TPP) is an essential cofactor for the function of numerous enzymes which are involved in central metabolism such as citric acid cycle, pentose phosphate pathway, Calvin cycle, isoprenoid biosynthesis, and branched-chain amino acid …
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Genetic engineering of antibody fragments for the detection of illicit drugs
The monitoring of illicit drugs is now of major importance. Key areas of analysis include the detection of illicit use by drivers, workplace testing, forensic investigations and general monitoring to detect or reduce abuse. Thus, the development of a highly sensitive and reliable array-based assay …
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Modification of bovine beta-casein using genetic engineering and its structure/function studies
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by Howard Ding (hding2@illinois.edu) on 2011-05-07T14:55:53Z Item is restricted indefinitely.
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Genetic Engineering of Black Cherry (Prunus serotina) for Reproductive Sterility and Insect Pest Resistance
<p>Black cherry (Prunus serotina Ehrh.) is one of the most valuable hardwoods for high- end cabinetry, furniture, architectural millwork, paneling, and veneer. However, the damage caused by cambial-mining insect pests triggers gummosis in black cherry, a non-specific defense response in which …
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Genetic engineering of recombinant anti-mycolic acid antibody fragments for use in tuberculosis diagnostics
… selected (MAC10) for further investigation by genetic engineering as presented in this dissertation. The antibodies of the Nkuku phage display library are in the format of single chain variable fragments (scFv). ScFv’s constitute only the epitope binding domains of an antibody consisting of the …
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Modelling and correction of retinal pathologies caused by mitochondrial DNA mutations via genetic engineering
… to progressive visual loss. Although advances in genetic engineering have enabled the modelling and correction of nuclear DNA mutations in the retina, challenges remain in modifying mtDNA. The generation of accurate models that mimic retinal mtDNA diseases and development of curative gene …
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Greenpeace cyberadvocacy: message strategies and the framing of the "say no to genetic engineering" campaign
… message characteristics and the framing of genetic engineering (GE) in news articles published in sites dedicated to two countries with different policy stances toward GE---Australia (precautionary) and Philippines (permissive).;The findings show the intent to generate awareness and …
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Bioethics and human rights in international law: genetic engineering, euthanasia and/or physician assisted suicide
… have arisen in regards to specific forms of genetic engineering, euthanasia and physician-assisted suicide. This dissertation is aimed at specifically focusing on the dichotomy of ideologies and legislation concerning the rights to human dignity and privacy in relation to the medical …
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