Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"Genetic Engineering Tools"”.
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Creating Genetic Engineering Tools for Investigating Bacillus anthracis.
… promoter region of <em>dnaB</em> to study the genetics of <em>B. anthracis</em>. An electro-transformation system was formulated to knock-out the -/- <em>B. anthracis</em> <em>dnaB</em> gene. We have successfully incorporated the pMUTIN4 vector into the chromosomal DNA of <em>B. anthracis</em>. …
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Engineering B Cells For Use In Immunotherapy and Cancer
… for cell therapy. We hypothesized that using genetic engineering tools we can skew the function of B cells toward a suppressive phenotype and support their ex vivo proliferation and survival to generate a viable subset of Bregs for cell therapy. We successfully generated B cells that displayed …
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Metabolic engineering of C. glutamicum for amino acid production improvement
A central goal in metabolic engineering is the design of more productive biological systems by genetically modifying metabolic pathways. In this thesis we report such an optimization in the bacterial strain Corynebacterium glutamicum that is employed for the fermentative production of various amino …
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Establishment of CRISPR-Cas-based gene deletions in Bacillus methanolicus and metabolic engineering towards the production of N-methylglutamate and putrescine
… methanolicus_ is limited by the lack of robust genetic engineering tools. Developing advanced genome editing technologies, such as CRISPR-Cas systems, is key to improving this organism for industrial applications and increasing yields and productivity in line with sustainability goals. The first …