Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"Genetic Code"”.
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Genetic Code Expansion in Mammalian Cells
… scientists have developed methods to expand the genetic code of living organisms, introducing new, non-canonical amino acids with diverse chemistries into proteins. These methods rely on engineering of the translational machinery often importing an aminoacyl-tRNA synthetase (aaRS)/tRNA pair that …
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Expanding the genetic code of a mouse
Genetic code expansion has used the site-specific insertion of unnatural amino acids into proteins(Greiss and Chin, 2011). An aminoacyl-tRNA synthetase and a tRNA are used to specifically insert the unnatural amino acid during mRNA translation, in response to an amber stop codon (UAG) placed at a …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… dynamic ZIKV particle. With this goal, I applied genetic code expansion to incorporate the non-canonical amino acid BCN-K at specific sites in the E protein. BCN-K is a versatile non-canonical amino acid that can be labelled via reactions with both tetrazine- and azide-conjugated probes. I …
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Synthesis, Characterisation and Evolution of Escherichia coli with a Compressed Genetic Code
… the creation of organisms with a compressed genetic code. Such liberated codons can be reassigned to non-canonical amino acids to equip proteins with novel functions. Furthermore, recoded organisms are genetically distinct from all other lifeforms and may therefore prove useful for …
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Identification and Evolution of New Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs for Genetic Code Expansion
Genetic code expansion is the branch of molecular biology aiming to expand the repertoire of amino acids which can be incorporated into proteins in vivo. A central challenge in expanding the genetic code of cells to incorporate non-canonical amino acids is the scalable discovery of aminoacyl-tRNA …
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Engineering genetic isolation in Escherichia coli and developing synthetic genomics tools to efficiently assemble megabase-scale genomes
… of E. coli allowed for the compression of its genetic code. However, DNA synthesis at the genome scale is not yet a mature technology. Our ability to synthesise genomes is still limited in both speed and scale. Chapter 2 - Refactoring the genetic code enables bi-directional genetic isolation …
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Stochastic many-body problems in ecology, evolution, neuroscience, and systems biology
… fluctuations: The evolutionary history of the genetic code, spatiotemporal pattern formation in ecology, spatiotemporal pattern formation in neuroscience and the robustness of a model circadian rhythm circuit in systems biology. In the first two research chapters, I demonstrate that the genetic …
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Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli
… in size and chemical properties. Expanding the genetic code to contain non-canonical amino acids (ncAAs) that contain functionalities not contained in nature is a powerful strategy for probing and extending the properties of proteins. Current in vivo systems for expanding the genetic code have …
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Computational studies of origins of life scenarios
… In particular, we focus on the origins of the genetic code and the intersection between geochemistry and a primordial ``biochemistry"" in which mononucleotides could form short oligoucleotide chains. We also apply quantum chemical methods to a modern biochemical reaction, the charging of tRNA …
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Engineering exclusively-quadruplet codon translation in vivo
Living organisms universally encode amino acids with three-base codons specifying the twenty canonical amino acids. A genetic code entirely based on four-base codons would answer many questions about the origin of life and have profound implications for expanding the genetic code to include novel …
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Urban Rhythms of Washington DC
… How does the architecture manifest this genetic code? I began to seek an architectural program where I could explore these questions. As a trained jazz drummer, I desired to create a place that could become a laboratory for jazz music and performance, while simultaneously serving the …
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A Parameterization Study of Short Read Assembly Using the Velvet Assembler
… of assembling large, contiguous sections of genetic code from short reads generated from laboratory techniques. We explore the Eulerian Path approach in detail, utilizing a de Bruijn Graph, and demonstrate current software technologies and algorithms using a sample genome. We investigate the …
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Studies on approaches for sequencing cytosine modifications and the identification of their protein readers
Alongside the genetic code, epigenetic mechanisms including covalent cytosine modifications facilitate dynamic changes in gene expression throughout development and differentiation. Such modifications are also drivers and markers of disease, earning them great academic and clinical interest. A …
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Intrinsic Disorder and Protein Evolution: Amino Acid Composition of Proteins in Last Universal Ancestor
… appeared early, while others were added into the genetic code later. The amino acids that were formed by Miller (1953) are suggested to have appeared early in evolutionary history, and the amino acids associated with codon capture developed late in the course of evolution. The chronological order …
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Collective Evolution of Biological and Physical Systems
… of the universality and optimality of the genetic code, and the emergence of genome biases. I present a new phase-field model of solidification which allows efficient computations in the regime when interface kinetic effects dominate over capillary effects. The asymptotic analysis required …
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A General Platform for Ribosome Engineering
… Emerging research aims to reprogram the genetic code in vitro and in vivo to assemble non-canonical polymers with this approach. However, the properties of the natural ribosome limit existing work to close analogues of the canonical amino acids. This thesis develops a directed evolution …
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The Role of Metabolism and Epigenetics in Regulating Cellular Plasticity
Eukaryotic cells encode genetic information in the form of DNA. However within our genetic code, only a subset of these genes are expressed in a given cell—giving rise to different cell types. The regulation of what genes are expressed is dictated by epigenetics. Epigenetics is the punctuation of …
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