Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"GeneChip"”.
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Inference on Quantile Regression for Mixed Models With Applications to GeneChip Data
The proposed test is motivated by studies of GeneChip data to identify differentially expressed genes through the analysis of probe level measurements. Realizing that the number of replicates is usually small in GeneChip studies, we propose a genome-wide adjustment to the test statistic to account …
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Development of a software infrastructure to mine GeneChip expression data and to combine datasets from different Medicago truncatula expression profiling platforms
… of thousands of genes in parallel. Affymetrix GeneChips®, more recently designed gene-specific chips, make it easier for the researcher to compare and evaluate gene expression and thus will most certainly lead to more accurate results. Not surprisingly, Medicago GeneChips® are moving into the …
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Widescale analysis of transcriptomics data using cloud computing methods
… analysis of public domain data for Affymetrix GeneChips which are a widely used technology for measuring gene expression. Methods to determine the bias in gene expression due to G-stacks associated with runs of guanine in probes have been explored via the use of a grid and various types of …
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Probe Level Analysis of Affymetrix Microarray Data
The analysis of Affymetrix GeneChip® data is a complex, multistep process. Most often, methodscondense the multiple probe level intensities into single probeset level measures (such as RobustMulti-chip Average (RMA), dChip and Microarray Suite version 5.0 (MAS5)), which are thenfollowed by …
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Gene expression prospective simulation and analysis unsing data mining and immersive virtual reality visualization
… relationships in biological processes. Current GeneChip microarray technology provides a platform to an- alyze up to 500,000 molecular reactions on a single chip, providing thousands of genetic and protein expression results per test. Using visualization tools and priori knowledge of genetic and …
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Non-parametric algorithms for evaluating gene expression in cancer using DNA microarray technology
… different research groups using the Affymetrix GeneChip^{TM} platform. A key finding of this study is that selectively expressed genes are very powerful when used for disease classification. A model was designed to reduce noise and eliminate false positives from true results. With this approach, …
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Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT
… genes in B. thetaiotaomicron , I used GeneChip and quantitative RT-PCR analyses. I showed that RteA and RteB are global regulators that induce the expression of various genes, in particular, tra genes in CTn, a cryptic CTn found in the chromosome. In addition, I characterized the …
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Dysregulation of Meox2 Following Wt1 Mutation In Kidney Development and Wilms Tumorigenesis
… following <em>WT1 </em>mutations, an Affymetrix GeneChip Human Genome Array was previously conducted to assess the differentially expressed genes in the <em>WT1</em>-wildtype human and <em>WT1</em>-mutant human WTs. Approximately 700 genes were identified as being significantly dysregulated. …
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Cellulose biosynthesis inhibitors modulate defense transcripts and regulate genes that are implicated in cell wall re-structuring in arabidopsis
… expression profiling based on Affymetrix ATH1 GeneChip arrays (contains 22810 probe sets) were applied to investigate the altered transcriptome of four different CBIs including CBI-15, 18, 22, and 27 and isoxaben in 5 day-old Arabidopsis thaliana seedlings. The results of this project revealed …
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BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF CMA-03/06, A NEWLY ESTABLISHED INTERLEUKIN-6 INDEPENDENT VARIANT OF THE CMA-03 HUMAN MYELOMA CELL LINE.
… data were generated by means of Affymetrix GeneChip Human Mapping 250K NspI arrays; copy number (CN) alterations were calculated using the DNAcopy Bioconductor package, based on circular binary segmentation method. Global gene expression profiling (GEP) was performed by means of the GeneChip …
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Recapitulation of Human Placental Insufficiency in a Novel Mouse Model :New Paradigm in Translational Research
… and 6) by ELISA and gene expression by qPCR and GeneChip microarray analysis. RESULTS: Compared with control, MUAL caused significant decrease (11%) in mean birth weight (1.06 ± 0.13 g vs. 0.94 ± 0.13 g, p<0.001) but no difference in placental weight (0.08 ± 0.03 g vs. 0.07 ±0.03 g,p=0.6). At 4 …
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Genetic analysis of Ximenia Americana under natural conditions
… to a well-known Arabidopsis thaliana genechip (ATH1). A. thaliana probe-pairs that hybridised to the X. americana genomic DNA on the basis of the perfect-match (PM) probe signal were selected and analysed using a cel file parser script to generate a new high density probe mask files. …
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A dynamic transcriptome technique for transcriptional profiling and gene regulatory network involving the helicase antigen (HAGE)
… profiling analysis of data gained from using the Genechip oligonucleotide microarray platform found significant changes to genes linked not only with proliferation but other cell processes altered during tumorigenesis. Confirmation using real-time qPCR suggested change in expression of certain …
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Host response of lung cells to virulent Bacillus anthracis
… infection with Ames spores was addressed using GeneChip analysis. Although few transcriptional alterations (15 genes) were detected in the lungs 8h post-infection, important inflammatory genes were both up- and down-regulated, indicating that the lung recognized microbial infection. After 48h, …
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Statistical methods for genetics and genomics studies
… for assessing RNA quality for a hybridized Genechip is proposed. In particular, our approach used the probe intensities that the Affymetrix software associates with each microarray. The effectiveness and improvements of the proposed method over the existing methods are illustrated by the …
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Characterization of sugar-insensitive mutants and analysis of sugar-regulated gene expression in Arabidopsis thaliana
… was undertaken using the Affymetrix GeneChip Arabidopsis ATH 1 Genome Array which includes over 24,000 Arabidopsis genes. Both wild-type germinating seeds and adult plants were studied in order to begin determining the impact of the developmental age of the plant on sugar-regulated …
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Analysis of Medicago truncatula transcription factors involved in the arbuscular mycorrhizal symbiosis
… Laser Capture Mirodissection and Affymetrix GeneChip® Medicago genome array hybridization. The establishment of cryosections facilitated the isolation of high quality RNA in sufficient amounts from three different cortical cell types. The transcript profiles of arbuscule-containing cells (arb …
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In vivo Genome-wide Expression Study on Human Blood B cells Suggests a Novel ESRI and MAPK3 Network for Postmenopausal Osteoporosis
… hybridized individually to Affymetrix HG-U133A GeneChip@ arrays to identify genes differentially expressed between low and high BMD females. Significance of differential expression was tested by t-test and adjusted with Benjamini and Hochberg (BH) procedure for multiple testing. Adjusted p S; …
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Disturbo bipolare e cefalea a grappolo: identificazione di geni e pathway molecolari e loro potenziale coinvolgimento nella risposta alla terapia con sali di litio tramite analisi dei profili di espressione genome‐wide
… Expression profiles were measured by Affymetrix GeneChip Human Gene ST 1.0 covering 36,079 transcripts. Expression levels of BD and CH patients were compared with CO using a t-‐test, in order to identify commonly dysregulated genes. Pathway analysis was performed based on the hypergeometric test …
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Identifying and characterizing genes that regulate vascular tissue-specific functions
… Affymetrix Arabidopsis ATH1 Genome Array (24K GeneChip), and identified potential genes that are required for xylem and phloem differentiation or tissue-specific functions. Based on this comparative analysis, two phloem-specific G2-like transcription factors, MYR1 and MYR2, and a xylem-specific …
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