Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 263 for “"Gene transfer"”.
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Agrobacterium-mediated gene transfer into kiwifruit
… that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from transformed plant cells to be assayed in the presence of Agrobacterium. Preliminary experiments examined the expression of these chimaeric GUS genes in …
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Understanding microbial communities through horizontal gene transfer
… the community assembly process under a serial transfer regime at a higher resolution and concluded that the diversity maintained is little affected by the complexity of the carbon source. Systematic variation of parameters in the regime demonstrated that diluting the initial inoculum has the …
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Factors that affect horizontal gene transfer in enteric bacteria
… through mutations or through horizontal gene transfer (HGT) of genetic material containing resistance genes. Conjugation machinery offers an efficient method for acquisition of AMR and virulence genes, which may be responsible for propelling the evolution of pathogenic bacteria. This …
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Engineering Secreted Proteins for Gene Transfer and DNA Vaccination
<p>In recent years gene therapy has become a promising way of alleviating incurable human ailments, its concept emerging as the ultimate therapy for many infectious and genetic diseases. Two important aspects of the development of successful gene therapy protocols are the ability to monitor gene …
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Factors affecting the efficiency of gene transfer in mice
… to: 1) test the best developmental stage for transferring injected embryos to obtain pregnancies and transgenic pups; 2) determine the optimum number of injected embryos transferred to obtain pregnancies and transgenic pups; 3) investigate whether addition of non-injected embryos with injected …
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HORIZONTAL GENE TRANSFER (HGT) AND SPHAGNUM FALLAX: A PHYLOGENETIC LOOK INTO THE OCCURRENCE, PREVALENCE, AND IMPORTANCE OF HORIZONTAL GENE TRANSFER IN A BOG MOSS
Horizontal gene transfer (HGT) is a biological process that involves any transmission of genetic material that isn't vertical in nature. HGT occurs frequently between prokaryotes and plays a significant role in prokaryotic evolution. This process is also known to occur between symbiotic bacteria …
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Horizontal gene transfer as a cohesive force in microbial populations
… defined as groups of organisms with a shared gene pool in which adaptive genes can spread freely through natural selection. Ecology takes a less abstract view of populations and conceives of them as members of a single species that occupy the same geographical area. Among sexual eukaryotes, …
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Development of a Gene Transfer System in Clostridium Scindens VPI 12708
… from the feces of a colon cancer patient. Many genes required for bile acid 7-a dehydroxylation are found on a large bile acid inducible operon (bai) that has been extensively studied. However the bai promoter, which directs expression of the bai operon, has yet to be characterized due, in part, …
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Gene transfer of keratinocytes with a human Factor XIII - plasmid construct
… thereby contributes to wound healing. Gene transfer offers an attractive method for localised and transient active delivery of factors to healing wounds. Aim of this study was the transient liposomal transfection of human keratinocytes with the gene for clotting factor XIII and the …
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Development and application of a gene transfer system in Clostridium perfringens
Initially, electroporation-induced transformation of intact cells of C. perfringens 3624A-Rif$\sp{\rm r}$/Str$\sp{\rm r}$ with plasmids pAM$\beta$1 and pHR106 resulted in transformation efficiencies of 1.4 $\times$ 10$\sp2$ and 1.2 $\times$ 10$\sp3$ transformants/$\mu$g DNA, respectively. An …
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Single cell analysis of bacterial communication and gene transfer by Enterococcus faecalis
… resistance. E. faecalis is particularly good at transferring antibiotic resistance by mechanisms like conjugation and conjugative transfer of plasmids can occur at a high frequency without antibiotic selection. Conjugative plasmid pCF10 encodes tetracycline resistance and transfer between E. …
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Bacterial transport and horizontal gene transfer in subsurface: multiple-scale experimental studies
Horizontal gene transfer rapidly changes bacterial genetic repertoire and contributes to bacterial evolution. Natural transformation, one mechanism of horizontal gene transfer, is defined as the process by which bacterial cells successfully take up and incorporate extracellular DNA. The …
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Development of in vitro regeneration and gene transfer systems for conifer species
… needles of S. sempervirens, shoot organogenesis and somatic embryogenesis were induced. The influence of genotype and various cytokinins (BA, BPA, and TDZ) in combination with 2,4-D were investigated. Among the cytokinins tested, 20 $\mu$M BPA with 5 $\mu$M 2,4-D produced the highest …
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Regulation of horizontal gene transfer by intercellular peptide signaling in Bacillus subtilis
Horizontal gene transfer plays an important role in bacterial evolution. Although acquisition of foreign DNA can be beneficial to cells, it can also be detrimental. Therefore, cells that possess mechanisms to regulate horizontal gene transfer likely have a competitive advantage. Similarly, several …
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Analysis of charged polymer effects on recombinant retrovirus-mediated gene transfer success
… efficiency of recombinant retroviruses in gene therapy experiments. Conversely, chondroitin sulfate proteoglycan is an anionic polymer endogenously present in retrovirus stocks which inhibits transduction efficiency. An experimental study was performed to determine the mechanisms of …
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Gene transfer history of carbon fixation proteins constrains marine cyanobacteria divergence times
… fixation proteins better informs the horizontal gene transfer event that brought carboxysomes from purple sulfur bacteria into marine cyanobacteria and that this gene history aids in constraining the evolutionary timing of carbon fixation. Genes encoding the proteins for the a-carboxysomal shell …
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Retroviral-mediated gene transfer of Il-1β?cDNA into human activated T cells
… malignancies or solid tumors. ATC are readily generated from peripheral blood and possess non-major histocompatibility complex (non-MHC) restricted killing properties. ATC are also powerful targets for genetic modification and hold promise for treatments of cancer, AIDS and T cell disorders. …
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