Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"Gene targeting."”.
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Gene-Targeting zur Herstellung einer IgE überexprimierenden Maus
Die hier vorliegende Arbeit stellt ein neues Mausmodell vor, mit dem der Einfluß von IgE in der Entstehung einer Allergie und die Folgen vermehrter IgE-Produktion in-vivo untersucht werden können. Erreicht wurde dies durch eine definierte Mutation im Erbgut von Mäusen. Mittels homologer …
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Improving Viral Vectors for Gene Targeting in Gene Therapy
… one out of every hundred live births (WHO). Gene targeting is a term that is used describe the manipulation of genetic material, either by adding a gene in a specific locus, creating a mutation at a specific locus, or correcting a gene at a specific locus. Here, unless otherwise noted, we …
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DEVELOPMENT OF TRAPPING STYLE CASSETTES FOR NEW GENE TARGETING STRATEGIES
… sequences to deciphering the functions of these genes. The 1980s saw the remarkable achievement of homologous recombination in mammalian cell culture systems. Later in the 1990s, innovative gene trapping strategies were developed to enabled random mutagenesis. Today, the goal is to generate more …
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Functional analysis of K-ras in the mouse by gene targeting
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Mu opioid receptor: construction of gene targeting vectors and mutagenesis for desensitization
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Examination of diverse physiological functions of mammalian heme oxygenases by gene targeting
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Gene Targeting in a Novel Mouse Model and the Chicken DT40 Cell Line
Gene targeting has the power to create precise changes at specific sites within the genome. In the context of gene therapy, this technology may be used to treat patients with monogenic diseases by fixing mutations in disease causing genes, followed by transplanting the corrected cells back into the …
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Investigation of isoform-specific fruitless mutants generated by gene targeting in Drosophila melanogaster
fruitless (fru) is a pleiotropic gene which produces an array of variant transcription factor isoforms to fulfil a range of developmental and behavioural roles, from the regulation of axonal pathfinding during embryogenesis, all the way to the precise orchestration of individual steps of the …
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Optimizing CRISPR/Cas9 Gene Targeting to Investigate Cone Photoreceptor Development in the Chick Retina
… result in the loss of vision, due to the degeneration of cone photoreceptors in the retina. Thus, the development of methods to regenerate these cone photoreceptors is an active area of study. In order to find ways to regenerate cones, the molecular pathways and gene regulatory networks that …
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Verwendung von Gene-Targeting-Techniken zur Etablierung neuer Mauslinien mit Mutationen in B-Zell-Signalwegen
… von CD22 zusammenhängen. Der „Targeting“ Vektor für die „Gene Targeting“ Experimente wurde von der Arbeitsgruppe Dr. Anton van der Merwe (von Christina Piperi) angefertigt. Ursprünglich wurde ein „Targeting“ Vektor aus genomischer C57BL/6-DNA verwendet, um den genetischen …
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Functional characterization of the cyclin-dependent kinase inhibitor p21 by gene targeting in the mouse
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1998.
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Optimizing Zinc Finger Nucleases for Use in Mammalian Cells
… being explored as a potential way of performing gene therapy. However, the spontaneous rate of homologous recombination in human cells is too low (10-6) to be therapeutically useful. The most powerful way of stimulating homologous recombination is by introducing a double strand break within the …
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Investigation into the role of DWNN in cell death
Many genes are activated to influence the self-destruction programme of the cell. This programme entails synchronised instigation and implementation of numerous subprograms. The arrival of gene targeting aided in the determining of the functions of novel genes. Such genes may have been sequenced, …
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In Vivo Genome Editing: Proof of Concept in Neonatal and Adult Mouse Liver
… vectors show great potential for therapeutic gene delivery for monogenic diseases, including hemophilia. Major limitations of this approach are the inability to persist in dividing cells and the restrictive packaging capacity of AAV. Gene targeting, the ability to make site-directed changes to …
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Studies of site-specific DNA double strand break repair in plants
… homologous recombination to achieve gene targeting in Arabidopsis. A defective visual-selective dual marker harboring <i> Ds </i> element is transformed into Arabidopsis as target locus, and donor homology is provided by Agrobacterium-mediated T-DNA by floral dipping. The DSB inducer …
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Genetic linkage of multiple modifications in the pig
Genetic engineering facilitates heritable modification of target genomes. Modifications can be site-specific or random. Though inefficient, homologous recombination allows for precise genome modification. Random integration can be very efficient, but lacks the opportunity to control the orientation …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
Gene therapy is the ability to correct diseases at the DNA level and has long been a goal of science and medicine. The earliest gene therapy clinical trial was for a patient with severe combined immunodeficiency (SCID) due to adenosine deaminase (ADA) deficiency. Initial trials looked promising and …
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Disruption of UHRF1 expression in chicken DT40 cells
Epigenetics regulate the gene expression while imposing no change in the underlying gene sequence constitution. The abnormal regulation of epigenetics is associated with the development and progression of cancer. Among the epigenetic regulators, UHRF1 (ubiquitin-like with PHD and ring finger domain …
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