Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"Gene fusions"”.
-
Identification and functional characterisation of gene fusions in human cancer cell lines
Advances in next-generation sequencing have accelerated the rate at which novel gene fusions are discovered. The discovery of gene fusions such as EML4-ALK in lung cancer and BCR-ABL1 in chronic myeloid leukaemia have already led to changes in clinical care. However, important questions remain …
-
A Systems Biology Approach to Characterizing Gene Fusion Pathways in Cancer
Gene fusions have long been known to drive cancer. Initial discovery of gene fusions was opportunistic, and functional assessment was done individually and experimentally. There is no comprehensive systems biology approach to understanding the impact of gene fusions on the signaling networks within …
-
Novel Oncogenic Drivers in Pediatric Gliomagenesis
… (NBS) tumors, from 118 patients using next-generation sequencing technologies. Nearly one-third of BS-HGGs, also known as diffuse intrinsic pontine gliomas (DIPGs), harbored somatic heterozygous missense mutations in <em>ACVR1</em>, coding for a receptor serine-threonine kinase involved in …
-
Analysis of the topology of the cytochrome d terminal oxidase complex of Escherichia coli by genetic methods
… were examined by the use of alkaline phosphatase gene fusions, and models proposed. The expression and assembly of the subunits was also examined. It was found that subunit II of the complex can be expressed independently of subunit I and associates with the cytoplasmic membrane. It does not …
-
The structure and evolution of breast cancer genomes
… as they can, for example, form fusion oncogenes or disrupt tumour suppressor genes. The recently described recurrent fusion genes in prostate and lung cancer proved to be iconic examples as they indicated that important gene fusions are found in the common epithelial cancers also. Breast …
-
Physical, genetic, and biochemical analyses of mannityl opine catabolism genes from the Agrobacterium Ti plasmid pTi15955
… utilization patterns identified at least six genetic units associated with catabolism of these opines. Functions for catabolism of MOP and mannopinic acid (MOA) are encoded by a 16.4 kb region, whereas those for AGR are encoded by a 9.4 kb region located within the MOP catabolic locus. The …
-
Genomic Discovery of recurrent CD44-SLC1A2 Gene Fusion in Gastric Cancer
… abnormalities associated with cancers, fusion genes and transcripts are particularly notable due to their cancer-specific nature and translational potential as diagnostic and therapeutic targets. While fusion genes are well-known in haematological malignancies, our knowledge of recurrent fusion …
-
Expression of atrial natriuretic factor in transgenic potatoes
… (Solanum tuberosum L. cultivar Iwa). Four ANF gene fusions were constructed for extracellular expression of ANF-(1-126) either by retaining the native signal peptide of ANF or replacing it with the secretory signal of a pathogenesis-related protein from tobacco. Two of the constructs were …
-
Electron Transport in Methanosarcina: Pathway Heterogeneity Within the Genus
Electron transport during methanogenesis in the freshwater methanogen Methanosarcina barkeri is known to utilize hydrogenases to transfer electrons from ferredoxin to methanophenazine. This thesis characterizes electron transport in the marine methanogen Methanosarcina acetivorans. Genomic …
-
Anc1 : a new player in the cellular response to DNA damage
… mechanisms to avoid and repair damage to their genetic material. In this thesis, we analyze a yeast gene that has a previously uncharacterized role in the cell's ability to survive after DNA damage. This gene, ANC1, is interesting for several reasons. First, ANC1 is the only common member of …
-
Studies on the Modular Evolution of Genes
Gene evolution is primarily studied through the observations of comparative cumulative point mutations between homologs. Genes also evolve through “remodelling”, the process of repurposing and reorganising genes and gene fragments into novel sequences. Gene remodelling is a relatively …
-
Integrative Analysis of Omics Data In Adult Glioma and Other Tcga Cancers to Guide Precision Medicine
<p>Transcriptomic profiling and gene expression signatures have been widely applied as effective approaches for enhancing the molecular classification, diagnosis, prognosis or prediction of therapeutic response towards personalized therapy for cancer patients. Thanks to modern genome-wide profiling …
-
Analysis of Methylotrophic Methanogenesis in Methanosarcina Barkeri Fusaro
… substrates to methane gas. Methylotrophic methanogenesis involves the disproportionation of methylated compounds, such as methanol, to methane and carbon dioxide. In this study, genetic in vivo analysis was utilized to characterize the C-1 oxidation branch of the methylotrophic pathway. It was …
-
Effect of Autoregulated TxeR on the Expression of <I>Clostridium difficile</I> Toxins
… kb toxigenic element that contains both toxin genes flanked by three small open reading frames (ORFs). The focus of this study is to elucidate the function of the ORF, designated txeR, which is located at the beginning of the toxigenic element. The deduced amino acid sequence of txeR predicts a …
-
The clinical value of genetic analyses of bone and soft tissue tumors
… is a need for ancillary techniques, such as genetic analyses, not only for diagnostic purposes, but also for obtaining information that could be useful for treatment and prognosis. Different types of bone and soft tissue tumors, both benign and malignant, were analyzed. The tumor material …
-
Biochemical and Genetic Characterization of Reduced Phosphorus Metabolism in Pseudomonas Stutzeri WM88
The first detailed biochemical and genetic analysis of reduced P metabolism in microorganisms is described in this thesis. Pseudomonas stutzeri WM88 is able to oxidize hypophosphite (P valence +1) and phosphite (P valence +3) for their use as the sole source of P. This process requires two operons …
-
Localization of a quinol oxidase domain of the cytochrome d complex of Escherichia coli
… across the membrane using $\beta$-galactosidase gene fusions is discussed, and a tentative topological model is proposed. Aspects of the model are confirmed in this work via proteolysis and antibody binding experiments. Previous studies characterized two monoclonal antibodies which bind to …
-
The Roles of the Germination-Specific Lytic Enzymes CwlJ1, CwlJ2, and SleB in Bacillus anthracis Spores
… in order to better understand their functions. Genetic manipulation of cwlJ1, cwlJ2, and sleB was fundamental to this work. First, reporter gene fusions revealed that all three are expressed during spore formation and that CwlJ1 is likely the most abundant GSLE in the spore. Second, gene …
-
Functional analyses of tomato 3-hydroxy-3-methylglutaryl coenzyme a reductase (HMGR) genes in transgenic plants engineered for altered HMGR expression
… at least four differentially regulated hmg isogenes encoding HMGR. Functions of tomato hmg2 in defense responses were studied by promoter analyses of hmg2:GUS gene fusions, overexpression of hmg2 cDNA, and antisense inhibition of hmg1 and hmg2 in transgenic plants. Activity of the hmg2 promoter …
-
Mechanistic and physiological insights into post-transcriptional regulation by small RNAs SgrS and DicF
… are important post-transcriptional regulators of gene expression in bacteria. These sRNAs deploy novel mechanisms to regulate mRNA targets leading to various physiological outcomes during stress conditions including, but not limited to, iron starvation, carbon flux and metabolism, virulence, and …
Page 1 of 2