Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Gene amplification"”.
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Gene Amplification for the Improved Secretion of Foreign Proteins in the Yeast Saccharomyces Cerevisiae
Gene amplification plays a central role in maximizing yield for protein production. Generally, more copies of a gene generally lead to an increase in protein production. The 2$\mu$ plasmid of Saccharomyces cerevisiae has been used extensively as a vector for foreign protein expression because of …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
Gene amplification in Drosophila follicle cells is an excellent model to study origin specification and developmental regulation of DNA replication in vivo. We mapped all follicle cell amplicons using a comparative genomic hybridization strategy and identified two new amplicons. We determined the …
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The Engineering of Chinese Hamster Ovary Cells to Achieve More Efficient Gene Amplification for Improving Biopharmaceutical Development
… The specific time consuming process of gene amplification is the focus of this work. Gene amplification is the process of selecting CHO cells which have been genetically modified to contain many copies of a therapeutic transgene, and therefore has the ability to produce a high amount of …
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Gene amplification in Drosophila ovarian follicle cells as a developmental strategy and model for metazoan DNA replication
The process of gene amplification in Drosophila ovaries provides a means of increasing the amount of template for transcription, thus increasing the amount of protein that can be made over a short developmental period. At a specific developmental point (egg chamber stage l0B-13), several clusters …
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Prognostic and Treatment Predictive Markers Associated with Cyclin D1 Gene Amplification and Epithelial-Mesenchymal Transition in Breast Cancer
… The markers examined were associated with CCND1 amplification (Chk1 and β-arrestin1), which has previously been linked to a potential adverse effect of tamoxifen, or with epithelial-mesenchymal transition (Snail), a biological process crucial for the development of metastasis. Immunohistochemical …
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Examination of target-site-based mechanisms of glyphosate resistance in waterhemp (Amaranthus tuberculatus)
… have been linked with GR waterhemp: EPSPS gene amplification, EPSPS target-site mutations, and reduced translocation. The objective of this research was to investigate the target-site-based mechanisms (EPSPS gene amplification and EPSPS mutations) of glyphosate resistance. Waterhemp …
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Evaluation of the P13K pathway and downstream effect in Her-2 positive and negative breast carcinomas
… continues to be a challenge due to the heterogeneity of the disease and the fact that individuals with the same stage and pathological diagnosis may respond differently to treatment as a result of differences in gene expression. Several components of the Her-2/PI3K/Akt pathway were evaluated …
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Negative feedback regulation as a means to constrain the oncogenic potential of mutant Egfr in NSCLC
… receptor could initiate tumor formation, we generated both a conditional and constitutive knock-in allele of one such mutation, L858R, at the endogenous murine Egfr locus. Expression of mutant Egfr failed to induce lung tumors in these mice; further analysis of the germline mutant mice …
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Molecular Genetics of Endocrine Induced Regression of Mouse Leydig Cell Tumors
… in malignant Leydig cell tumor formation in genetically susceptible strains of mice. The hypothesis that the mechanism of estrogen induced carcinogenesis includes a gene amplification event was tested using this<em> in vivo</em> endocrine carcinogenesis model. High molecular weight DNA …
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A retrospective histopathological study and selected molecular genetics of archival prostatic cancer tissue
… a method to determine androgen receptor (AR) gene amplification in formalin fixed prostatic carcinoma tissue.
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Identification Of Neuroblastoma And Its Prognostic Markers Using Raman Spectroscopy
… aggressiveness can also be judged based on the amplification of certain genes, including MYCN. Raman spectroscopy is a physics-based method which identifies the biochemical fingerprint of a sample. It has recently been applied to disease classification, specifically in adult cancers.</p> …
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Profilo molecolare dei linfomi post-trapianto
… of the tissue core. FISH analysis detected BCL2 gene amplification and MYC rearrangement. EBV was identified in 32 cases (64%) performing immunohistochemistry (LMP-1) and in situ hybridization (EBER). Clinical data and follow-up were available in all cases of malignant lymphomas but one. …
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Mechanisms Underlying Distinct Egfr Versus Fgfr-3 and -1 Dependency In Human Bladder Cancer Cells
… growth factor receptor (FGFR) are activated by gene amplification, mutation and overexpression in bladder cancer, which drives tumor development and progression. Both EGFR and FGFR inhibitors are currently being tested in clinical trials. However, bladder cancer (BC) cells show remarkably …
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Structure of the amplified 5-enolpyruvylshikimate-3-phosphate synthase gene in glyphosate-resistant carrot cells and attempts to isolate the anthranilate synthase gene from plants
… was carried out to determine the mechanism of gene amplification in this plant system. Cloning of the EPSPS gene and 5$\sp\prime$ flanking sequences was carried out and two different DNA structures were revealed. The 13 kbp clone contained only one copy of the EPSPS gene while the 16 kbp clone …
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Developmental regulation of DNA replication in Drosophila melanogaster
… cell cycle proceeds without complete mitosis, generate polyploidy and are commonly employed to increase metabolic capacity and cell size. D. melanogaster follicle cell gene amplification, in which bi-directional replication occurs in the absence of detectable gap phases, serves to produce large …
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Part I: Steroid Receptor CO-Activator 3 (SRC-3) Expression in Lung Cancer and its Role in Regulating Cancer Cell Survival and Proliferation; Part II: Development of Phosphospecific Peptoid Ligand
… breast cancer, however, its role in lung tumorigenesis is unknown. We found that SRC-3 is over-expressed in 27% of the NSCLC patients, and SRC-3 high expression correlates with poor disease-free survival and overall survival. We also studied DNA copy number, mRNA and protein expression of SRC-3 …
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Developmental regulation of DNA replication initiation in Drosophila
Developmental gene amplification in the ovarian follicle cells of Drosophila provides a powerful system for the study of metazoan DNA replication. Amplification produces 100kb gradients of amplified DNA through repeated rounds of origin firing and bidirectional movement of replication forks from …
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Surface-Enhanced Raman Spectroscopy Enabled Microbial Sensing
… Current analytical methods for microbes are generally either culture-based, gene amplification-based, or sequencing-based. However, these approaches require centralized facilities, well-trained personnel, and specialized instruments that result in high costs and long turnaround times. …
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CHARACTERIZATION OF GENETIC AND EPIGENETIC MODIFICATIONS IN A MODEL OF INFLAMMATION-DRIVEN CANCER
… yet unclear whether inflammation could induce epigenetic modifications, and if these changes are relevant to tumor generation. Therefore, the aim of this work was to assess inflammation-derived genomic and epigenomic modifications at multiple stages of tumorigenesis in Mdr2-knockout mice, a model …
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Mechanisms of Palindrome-Mediated Chromosomal Instability
… instability by defining structural and genetic determinants of palindrome fragility. I discovered that palindromic sequences with nonpalindromic spacers of 8 bp or less form stable hairpins which can block replication, and these hairpins are cleaved by the Mre11/Rad50/Xrs2/Sae2 complex. …
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