Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Gene Synthesis"”.
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Microfluidic gene synthesis
… is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications in both traditional molecular biology and the emerging field of synthetic biology, from the synthesis of large sets of novel proteins to the complete re-writing of …
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Towards automatic gene synthesis with bioinformatics software, novel one-step real-time PCR assembly, and lab-chip gene synthesis
This PhD thesis presents the whole process of gene synthesis method development and optimization, including the development of bioinformatics software TmPrime, TopDown and Automatic TouchDown one-step gene synthesis methods; and based on the developed protocols, this thesis also demonstrates an …
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Oligonucleotide design and codon optimization for PCR-based gene synthesis
… An optimal set of oligonucleotides for PCR-based synthesis has no potential for mis priming and has maximally uniform overlap melting temperatures. We present an algorithm that finds such a set. Unlike similar algorithms, it places no restrictions on oligo length or overlap size except those given …
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DNA as a programmable material : de novo gene synthesis and error correction
… (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in …
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A systematic and extensible approach to DNA primer design for whole gene synthesis
… development of accurate and inexpensive whole gene synthesis technologies. Recent advances in the purification of solid-phase manufactured oligonucleotides make it possible to manufacture whole genes by polymerase chain reaction methods. Yet, despite the improvement in laboratory methods, whole …
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Transmembrane glycoprotein gp41 of the Human Immunodeficiency Virus Type I : gene synthesis, recombinant expression and immunological characterization
… The endo-domain was truncated into three gene fragments namely gp41T3 (405 bp), gp41T2 (591 bp) and gp41T1(696 bp), all containing the Kennedy domain located on the C-terminal of gp41. The 80 amino acid fragment gp41(80) of the ectodomain, located on the N-terminal of gp41 was also …
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Total gene synthesis, bacterial expression and functional characterization of a recombinant human hemoglobin: Progress towards a blood substitute
… and protein-protein recognition, a genetic handle is required. The use of total gene synthesis alleviates some of the laborious procedures associated with conventional cDNA cloning and circumvents the problems associated with the expression of human hemoglobin chains from cDNA …
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Synthesis and error correction methods in gene fabrication
Gene Fabrication technology involves the development and optimization of methods relevant to the in vitro synthesis of any given target gene sequence(s) in the absence of template. The driving purpose of this field of research is to bring about the capability for on-demand fabrication of a DNA …
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Production and characterization of MutS for use in error correction
… DNA (oligonucleotides) has allowed for the synthesis of longer, gene-length constructs of DNA. However, a critical barrier to making this technology a low-cost and high-throughput process has been due to the rate at which errors pervade the final product. The current state of the error …
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Resource Allocation and Process Improvement of Genetic Manufacturing Systems
… molecular and synthetic biology through de novo gene synthesis are stimulating new vaccines, pharmaceutical applications, and functionalized biomaterials, and advancing the knowledge of the function of cells. This evolution in biological processing motivates the study of a class of manufacturing …
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Expression of single chain antibody-encoding genes in fission yeast and tobacco
… of this project was to explore the expression of genes encoding single chain antibodies (SCA) in eukaryotic cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed for the …
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A study of the molecular mechanism of the attenuation response in the threonine operon of Escherichia coli by synthesis of mutant attenuators
… of position 165 as an artifact arising from the gene synthesis ligations.
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Photoelectromechanical synthesis of low-cost DNA microarrays
Recent advances in de novo gene synthesis, library construction, and genomic selection for target sequencing using DNA from custom microarrays have demonstrated that microarrays can effectively be used as the world's cheapest sources of complex oligonucleotide pools. Unfortunately, commercial …
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Intrinsically Disordered Protein Polymer Libraries as Tools to Understand Protein Hydrophobicity
… that exhibit phase transitions consists of gene synthesis and protein screening. We developed two key technologies that has enabled (1) the scalable synthesis of repetitive gene libraries using a novel graph theoretic gene optimization approach (Codon Scrambling) and (2) the pooled synthesis …
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Large-scale production and characterization of an engineered human olfactory receptor
… proteins in milligram quantities. Expressed OR genes were fabricated from scratch using PCR-based gene synthesis, which facilitated codon optimization and attachment of different affinity tags for purification. Established methods for the production and purification of rhodopsin were adapted to …
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Identifizierung und funktionelle Analyse der humanen Xylosyltransferase I- und II-Promotoren
… and rate-limiting step in glycosaminoglycan biosynthesis. Both XT isoforms represent key enzymes in extracellular matrix assembly and remodeling and do also play a major role in various pathological processes. The xylosyltransferases show a high degree of homology in structure and function. …
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PCR-based Synthesis of Codon Optimized cry2Aa Gene for Production of Shoot and Fruit Borer (Leucinodes orbonalis) Resistant Eggplant (Solanum melongena L.) Cultivars
… cultivars that express a codon-optimized cry2Aa gene, the sequence of which is based on that of an Indian isolate of Bt, with the eventual goal of producing fully resistant cultivars. The cry2Aa gene was modified for optimal expression in eggplant using the codon usage frequencies based on …