Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"Gene Knockout"”.
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Gene Knockout Of PRFA And The Arginine Deiminase Pathway In Listeria Monocytogenes
<p>Listeria monocytogenes is a Gram-positive facultative intracellular pathogen. This species is a common cause of food-borne illness outbreaks with high fatality rates. L. monocytogenes is known to produce a number of virulence factors, including enzymes and toxins, which are involved in the …
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Efficiency of the TargeTron Gene Knockout System as a Transformative Protocol for the Mutagenesis of Listeria monocytogenes
… employed in labs worldwide. Electroporators are generally small and relatively inexpensive, making them attractive systems to use for a variety of purposes. Electroporation protocols are numerous in the published literature and encompass all cell types, from prokaryotic bacterial cells to …
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Genetic mapping of novel mutation, UV90, and its effects on the Neurospora crassa circadian system
… conidiation of the FLO, and locating this gene will lead to better understanding of the FLO mechanism. Mapping of the UV90 gene location was performed using Cleaved Amplified Polymorphic Sequence (CAPS) markers and gene knockout screens. DNA sequencing and complementation tests were …
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Development of systematic and combinatorial approaches for the metabolic engineering of microorganisms
… was employed to identify single and multiple gene knockout targets for lycopene production in E. coli. These targets led to substantial increases in lycopene production, but were limited in scope due to the nature of these models. Therefore, these approaches and targets were complemented with …
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The IN4MER CRISPR/Cas12a Multiplex Knockout Platform and Its Applications
… synthetic lethal interactions between genes holds the key to uncovering cancer vulnerabilities, enabling the development of more effective drugs for patients. However, identifying these vulnerabilities in the complex genome of human, which comprises thousands of genes, poses a …
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Significance of DNA base excision repair in the maintenance of mitochondrial function in Saccharomyces cerevisiae /
… Saccharomyces cerevisiae (S. cerevisiae) BER gene knockout strains, including 3 single DNA glycosylase gene knockout strains and Ap endonuclease (Apn 1 p) knockout strain were used to examine the importance of this DNA repair pathway to the maintenance of respiratory function. Here, I show …
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Exploiting Chemogenetic and Genetic Interactions In Human Cells As An Avenue For New Therapeutic Opportunities
… to the mammalian genome made whole-genome knockout screens possible directly in human cells. Gene knockout answers how essential that gene is for cell fitness and proliferation. Genes showing moderate to severe fitness defects are called essential genes and provide insights into …
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Mechanistic Dissection of Tcf7l1-Dependent Enhancer Decommissioning During Exit from Naïve Pluripotency
… the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at Tcf7l1-bound cis-regulatory elements in …
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Mosaic Analysis with Double Markers (MADM) as a Method to Map Cell Fates in Adult Mouse Taste Buds.
… approached this issue from either cell cycle gene expression properties or lineage tracing of precursor cells. In our study, we apply a new fate mapping technique that combines these two ideas. This technique, Mosaic Analysis with Double Markers, allows for simultaneous gene knockout and …
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Investigations of lipid metabolism in Yarrowia lipolytica
… mediated by the peroxisomal oxidases, or POX genes. These enzymes catalyze the formation of a trans double bond, producing the trans-2-enoyl product. Our study looked at the comparison of the Y. lipolytica prototrophic strain against a knockout of the Pox2 gene on the uptake, incorporation, …
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Genetic screens of RNA binding proteins identify a role for N6-methyladenosine in promoting B cell differentiation
Post-transcriptional regulation of gene expression is mediated in part by RNA binding proteins (RBPs). Roles for RBPs have emerged in the immune system but remain largely unexplored. My project utilised high-throughput genetic screens to identify roles for RBPs in lymphocytes. Initially, I …
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The role of BELL transcription factors in tuber induction of potato (Solanum tuberosum)
… tips-is regulated by a complex interplay of genetic, metabolic, and environmental factors. While environmental influences such as photoperiod, temperature, and nitrogen availability have long been recognized, the underlying molecular mechanisms are still being uncovered. Given that potato is …
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OPTIMISATION AND THERAPEUTIC-TARGET-DISCOVERY ORIENTED ANALYSIS OF CRISPR-CAS9 SCREENS
… are vital in cancer research, allowing precise gene knockout and unveiling genes critical for cancer cell survival. However, challenges persist in experimental setup optimization and data analysis. My work addresses these challenges by optimizing CRISPR-Cas9 screens and developing advanced …
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Control of Cardiac and Limb Development by the bHLH Transcription Factors DHAND and eHAND
… and overlapping patterns during embryogenesis, and gene knockout studies have demonstrated that dHAND and eHAND null embryos die from defects in right ventricular and placental development, respectively. Therefore, we have investigated three aspects of HAND gene biology. Firstly, in …
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Genetic linkage of multiple modifications in the pig
Genetic engineering facilitates heritable modification of target genomes. Modifications can be site-specific or random. Though inefficient, homologous recombination allows for precise genome modification. Random integration can be very efficient, but lacks the opportunity to control the orientation …
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Transcriptomic and proteomic analysis of lycopene-overproducing Escherichia coli strains
… of both rationally-directed and combinatorial gene knockout strains of E. coli recombinantly producing the small molecule lycopene. Global genomic and proteomic expression changes associated with increased lycopene production of mutant E. coli constructs were discovered using whole-genome DNA …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… pathways and cellular migration. Current genetic strategies for the study of phosphorylation, including gene knockout and point mutation, are limited in providing temporal information. As a complement to these techniques, the synthesis and semisynthesis of probes that enable researchers to …
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Pathogenic and antigenic characterization of <I>Neospora hughesi</I>
… the susceptibility of BALB/c gamma-interferon gene knockout (gamma-INFKO), BALB/c, CD-1, and C57BL/6 strains of mice and gerbils to infection with tachyzoites of the Nh-A1 strain of <I>N. hughesi</I>. Only the gamma-IFNKO mice developed severe clinical disease following infection with <I>N. …
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Simulation and identification of gene regulatory networks
Gene regulatory networks are a well-established model to represent the functioning, at gene level, of utterly elaborated biological networks. Studying and understanding such models of gene communication might enable researchers to rightly address costly laboratory experiments, e.g. by selecting a …
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The Role of SOX2 in Molecular Circadian Clock Regulation and Suprachiasmatic Nucleus Development
… of my doctoral research, Sox2, is a stem cell gene that has been extensively studied for its function in stem cell maintenance and proliferation, cellular reprogramming, neurogenesis, and tumorigenesis. Sox2 is expressed and maintained in stem cells of different tissues, including the …
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