Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"Gene Discovery"”.
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Gene discovery in Catharanthus roseus using virus induced gene silencing
This thesis presents the use of Virus Induced Gene Silencing (VIGS) for the discovery of enzymes and transporters involved in monoterpene indole alkaloid (MIA) metabolism in the medicinal plant Catharanthus roseus. C. roseus is the source of a number of MIAs that are used as chemotherapeutic agents …
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Fine Mapping and Candidate Gene Discovery at the Rsv3 Locus
… of single dominant, strain specific resistance genes, referred to as Rsv genes. Rsv1 is the most widely used form of SMV resistance with nine different alleles conferring resistance only to the lower numbered less virulent strains, G1 to G3. Rsv3 gives resistance to higher numbered more virulent …
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Gene Discovery In Nonsyndromic Cleft Lip With Or Without Cleft Palate
… year in the United States, is caused by both genetic and environmental factors. Orofacial clefts affect the mouth and nose, causing severe deformity of the face, which require medical, dental and speech therapies. Despite having substantial genetic liability, less than 25% of the genetic …
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Developing molecular genetic tools for gene discovery in soybean (Glycine max)
… and highly duplicated, with ~75% of predicted genes present in multiple copies, and 50% of these paralogs are sub-functionalized. In addition, the relatively low genetic diversity in soybean relative to other major crops (e.g., corn) limits the available phenotypic variations that can be …
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SIMPLIFIED TRAIT AND GENE DISCOVERY FOR DROUGHT RESILIENCE IN WHEAT USING EINKORN WHEAT
… and abiotic stresses, particularly drought. Genetic improvement remains the most sustainable strategy for enhancing stress resilience and yield stability; however, gene discovery in bread wheat is complicated by its allohexaploid genome (AABBDD, 2n = 6x = 42) and extensive genetic redundancy. …
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Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.
… the 3’ end of the dopamine D2 receptor (DRD2) gene; Catechol-O-Methyl Transferase (COMT) NlaIII high/low activity polymorphism; 5-HT2A 1438 A/G polymorphism; 5-HT1Dβ G861C polymorphism and the 5-HTTLPR 44-bp deletioninsertion. There was a higher frequency of the DRD2 A2 allele (p = 0.020) and …
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Gene discovery and expression profilng in the toxin-producing marine diatom, Pseudo-nitzschia multiseries (Hasle) Hasle
… initiate characterization of actively expressed genes that control cell growth and physiology in P. multiseries, with the specific goal of identifying genes that may play a significant role in toxin production. The first step in gene discovery was to establish a complementary DNA (cDNA) library …
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Gene discovery and expression profiling in the toxin-producing marine diatom, Pseudo-nitzschia multiseries (Hasle) Hasle
… initiate characterization of actively expressed genes that control cell growth and physiology in P. multiseries, with the specific goal of identifying genes that may play a significant role in toxin production. The first step in gene discovery was to establish a complementary DNA (cDNA) library …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… of this thesis was to identify TCDD-responsive genes likely to be involved in processes of toxicity. We constructed microarrays using cDNA libraries derived from zebrafish embryonic and adult heart tissue. Embryonic heart arrays were used for protocol development. The resulting workflow was …
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Assessing wild barley germplasm in multiparent and advanced backcross populations for mapping, gene discovery, and improvement of malting barley
… vulgare ssp. vulgare), is a rich source of genetic diversity. This diversity is not easily exploited due to challenges inherent in identifying and extracting beneficial alleles from widely unadapted germplasm. Wild barley germplasm has been shown to contain valuable alleles for several …
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Generation of cDNA chips from the black widow spider, latrodectus hesperus, for gene discovery and expression profiling using microarray technology, and molecular characterization of a novel silk glue protein
<p>eDNA microarray technology has generated a tremendous amount of interest among biologists because of its promise to monitor the entire genome on a single chip, thus enabling researchers to have a better picture of the interaction among thousands of genes simultaneously. In the current study, …
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Gene prioritization through hybrid distance-score rank aggregation
… developing novel rank aggregation methods for gene prioritization. Gene prioritization refers to a family of computational techniques for inferring disease genes through a set of training genes and carefully chosen similarity criteria. Test genes are scored based on their average similarity to …
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Identifying New Genes for Inherited Breast Cancer by Exome Sequencing
… risk factor for its occurrence. More than 20 genes have been identified with inherited mutations that lead to significantly increased risk of breast cancer. However, most familial breast cancer is not explained by mutations in these genes. The goal of this project was to identify additional …
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Genetic determinants underlying rare diseases identified using next-generation sequencing technologies
… families and the health care system. Gene discovery is the starting point in understanding the molecular mechanisms underlying these diseases. The advent of next-generation sequencing has accelerated discovery of disease-causing genetic variants and is showing numerous benefits for …
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The developmental liver transcriptome of Rattus norvegicus
… biological stages. We investigated such areas as gene expression, isoform expression, transcriptional regulation, and post-transcriptional modifications of transcribed molecules. Furthermore, we used a bioinformatics approach to improve upon the reference annotation for known genes. Our research …
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Improvement of a high throughput method for identification of T-DNA insertion events in an activation-tagged population of Populus tremula x Populus alba.
… are a cornerstone of modern studies in genetics; their usefulness in gene discovery, and in understanding the various forms of gene regulation, can not be overstated. Since the introduction of mutation in plants nearly a century ago (Stadler, 1928), mutant populations have been generated …
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Knowledge discovery and modeling in genomic databases
… effective and accurate methods for identifying gene structures in the genomes of high eukaryotes, such as vertebrate organisms. Several effective hidden Markov models (HMMs) are developed to represent the consensus and degeneracy features of the functional sites including protein-translation …
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Uncovering novel genes causing isolated gonadotropin releasing hormone deficiency using runs of homozygosity in outbred families
… puberty and infertility. Over 30 causal IGD genes have been discovered, yet only 30-35% of IGD cases have a proven genetic etiology, highlighting the importance of new discovery methods. Homozygosity mapping, traditionally used to detect autozygous segments in inbred populations, has recently …
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Identification and Characterization of Modulators of Chemotherapeutic Response in Mouse Models of Cancer
… While much attention has been focused on the genetics of tumor development, less is known about the genetic determinants of therapeutic outcome. As a central focus of my graduate work, I have employed pool-based shRNA-mediated screening methodologies using the Ep-myc lymphoma model to identify …
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