Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Gene Circuit"”.
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RNA sensing and programming platforms for mammalian synthetic Biology
… to control cellular behavior using programmable gene circuits. Generally these gene circuits sense molecular biomarkers, process these inputs and execute a desired calculated response. This is especially relevant for gene and cell therapies where integrating multiple disease-related inputs and/or …
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Self-organized Pattern Formation using Engineered Bacteria
… gradients, which are either predefined or generated as part of the patterning processes. However, using E. coli programmed by a simple synthetic gene circuit, I demonstrate here the generation of robust, self-organized ring patterns of gene expression in the absence of an apparent morphogen …
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RSMLab : a web-based tool for recombinase-based state machine design and visualization
… an intuitive web-based application for creating circuits that implement state-dependent logic in living cells using our scalable state-machine framework. Through a graphical user interface, RSMLab users choose a desired state diagram, define arbitrary genes, and specify whether those genes are on …
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Functional Analysis of Synthetic Gene Circuits Controlling A Protein Pump In Yeast
… systems and explore new applications. Synthetic gene circuits such as genetic switches, oscillators and logic gates are at the core of many synthetic biology applications. These gene circuits often include a sensor/regulator protein capable to detect small molecules and then transduce them into a …
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… vector, this is referred to as a DNA library. Generating quality DNA libraries that incorporate broad genetic diversity is challenging, despite the availability of published protocols. In response, a novel, efficient, and reproducible technique for creating DNA libraries was created in this …
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Towards dissecting neural ensembles : development of genetic profiling and targeting approaches
The recent development of genetic neural modulation technologies has brought about a renaissance in systems neurobiology, where manipulation of specific neural ensembles coupled with measurements of the resulting behavioral changes have begun to chart the functional organization of the brain. …
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The Role of Gene Expression Noise In Mammalian Cell Survival
… contributing to this problem is cellular heterogeneity. Even in the same environment, cells with identical genomes can display cell-to-cell differences in gene expression, also known as gene expression noise. Gene expression noise can vary in magnitude in a population or in fluctuation time …
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Improving Bacterial Therapeutics and Diagnostics through Synthetic Biology
… could be used to achieve kinetic control in gene circuit design. This high-throughput, data-driven method enhanced our understanding of recombinase function and expanded the synthetic biology toolbox, and it can enable rational tuning of the response dynamics of bacterial diagnostics and the …
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Bistability, Synthetic Biology, and Antibiotic Treatment
… focused primarily on cellular mechanisms that generate bistable switches and their impact on cellular dynamics. </p><p>Here, I study emergent bistability due to bacterial interactions with either synthetic gene circuits or antibiotics. A synthetic gene circuit is often engineered by considering …
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Dynamic localization of chemotaxis proteins in Bacillus subtilis
… proteins found up to now and represents a general model for chemotaxis in bacteria. In this work, we investigated the dynamic localization of the chemotaxis signaling arrays in B. subtilis using immunofluorescence. We found that the receptors were localized in clusters at the poles in most …
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Design of genetic regulatory networks
… thesis, we present two approaches for designing genetic regulatory networks in the field of synthetic biology. The core problem is to capture topological properties and dynamical mechanisms in such a way that enables generation of large-scale and complex genetic regulatory networks. Synthetic …
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Development of a reliable screening platform for PET hydrolysing enzymes in an ultra-high throughput setup
… Since PHEs stem from different enzyme families general findings on one PHE cannot easily be transferred to other known PHEs. Thus functional assays that are compatible with high throughput screens would enable far larger explorations of protein sequence space and thus increase the probability to …
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Multiscale modeling of microbial communities
… array of social interactions through regulatory gene networks. The social interactions between individual cells drive the emergence of community structures, resulting in an intricate relationship across multiple spatiotemporal scales. Here, I present my work towards developing and applying the …
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Integrated modeling of bacterial metabolism
… into three modules (metabolic reactions, gene regulation, and environmental cues), which are later developed separately and assembled together through their input-output relationships. Parameters are identified by reproducing fermentation profiles of the wild-type strain and its several …
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THE CRISPRATOR: A FEEDBACK CONTROLLER IN MAMMALIAN CELLS FOR ROBUST PERFECT ADAPTATION
Synthetic Biology, merging genetic engineering with control theory, aims to achieve predictable behaviours in living systems, not relying on single components but rather on their interactions. Viewing DNA, RNA, and proteins as electronic circuit components, Synthetic Biology seeks to emulate …