Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Gene Array"”.
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Defining the ethanol-stress response in Saccharomyces cerevisiae
… level by the induction of stress response genes. While there have been several investigations into the effects of ethanol on yeast, few have focused on the underlying genetic mechanisms that enable yeast cells to tolerate and adapt to this stress. This thesis used differential display and …
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Early divergent B cell differentiation during antibody responses
… and switched antibody protein in parallel with gene array analysis and single cell real time RT-PCR in responding B cells was used to identify GC and plasmablast precursors and when and where CSR occurred. The results suggest CSR occurs in B blasts before GC B cells or plasmablasts emerge in …
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Co-factors of LIM-HD transcription factors in neural development and axon pathfinding in zebrafish
… of LIM-HDs) can activate LIM-HDs and downstream gene transcription while over-expression of dominant-negative CLIM (DN-CLIM), which lacks the DD domain, blocks LIM-HD activity. Motor neurons fluoresce in HB9:GFP transgenic zebrafish as the promoter of the motor neuron specific gene Hb9 drives …
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Dynamics of vascular normalization during anti-angiogenic therapy : implications for combination therapy
… blood cell velocity after DC101 treatment. Using gene array, real time PCR and Western blot analyses, changes in angiopoietin-2 level during DC101 treatment are identified. To test if similar effects happen in clinical setting, we obtained tumor biopsy samples from rectal adenocarcinoma patients …
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Gene expression profile of ethanol-stressed yeast in the presence of acetaldehyde
… for improving ethanol tolerance in yeast. Gene array analysis was used to study gene expression in Saccharomyces cerevisiae during acclimatisation to non-lethal ethanol stress, in the presence and absence of acetaldehyde. Acetaldehyde caused significant changes in gene expression in …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
… It has been previously shown that the DHX36 gene product, G4-Resolvase1 (G4R1), binds parallel tetramolecular G4-DNA with high affinity and resolves these structures into single strands. Here we show that G4R1 specifically binds to a parallel, unimolecular G4-DNA with remarkable affinity, …
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Mechanisms of Induction and Maintenance of Constitutive Ptgs2 Expression in Colonic Mesenchymal Stem Cells
… of Ptgs2 expression in cMSCs. Subsequent global gene array analysis comparing these cMSCs to a similar cell isolated from the bone marrow revealed a number of candidate genes with potential roles in post-transcriptional regulation of Ptgs2 expression. We determined that Fgf9 can regulate Ptgs2 …
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Understanding Acquired Resistance to Lapatinib In Breast Cancer Cells
… indicated higher expression of STAT3 target genes, as well as of other genes that promote survival. The gene array data also revealed cell cycle regulators and cell adhesion/junction component genes as possible mediator of Lapatinib resistance. Altogether, this study has identified several …
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CD46-mediated signals in the regulation of T-cell cytokine production and intestinal wound healing
… are largely undefined and were investigated. Gene array studies highlighted the asparagine endopeptidase (AEP) as a candidate molecule involved in the regulation of the switch of TH1 cells towards IL-10 production. The CD46-driven IFN-y secretion, but not the intracellular expression, was …
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Development of Techniques for Analysis of the Human Retinal Ganglion Cell Transcriptome: Application to the Role of Calcium in RGC Death in Glaucoma
… neuronal markers validated the technique. Global gene expression analysis, using Illumina arrays, compared expression in the retina ganglion cell layer (RGCL) and entire macula (Mac). Immunohistochemistry and QRT-PCR validated gene array data. RGC death was investigated using a simulated ischemia …
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Development of a novel approach to expand umbilical cord blood-derived haematopoietic stem/progenitor cells ex vivo for clinical transplantation
… GSK-3 inhibition on progenitor function while gene array technology was used to investigate potential molecular mechanisms. We have shown that expansion of UCB HSPC results in a loss of primitive phenotype, an increase in progenitors with reduced function and a shift in transcriptional profile …
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The Role of Slow-Twitch Skeletal Muscle in the Musculoskeletal Endocrine Axis
… muscle contractile force, muscle size and regeneration of slow type-1 muscle fibers compared to control littermates while EDL muscle properties remained largely unaffected. Subsequent gene array analysis uncovered upregulation of genes related to muscle contraction, oxidative metabolism and …
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The Expression of the Zinc Finger Transcription Factor zDC Defines the Classical Dendritic Cell Lineage
… and more specific cDC marker, I compared the gene expression profiles of differentiated cDCs, committed cDC precursors (pre-DCs), monocytes, and myeloid progenitor populations by gene array. Enriching cDCspecific genes identified an uncharacterized zinc finger transcription factor that I call …
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A Systems-Based Approach to Examine the Molecular Basis of Interactions between Human Monocytes and Human Micro-Vascular Endothelium
… these differences were further investigated by gene array analysis of micro-vascular endothelium under resting and inflammatory conditions. In a limited number of clinical cases, endothelial cells were isolated from RA synovium and characterised by gene expression microarray. These data showed …
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Regulation of VEGF-activated signalling by the plasma membrane calcium ATPase 4 in endothelial cells
INTRODUCTION: Angiogenesis, the formation of new blood vessels from pre-existing ones. It is a tightly regulated processes involving pro- and anti-angiogenic molecules. Deregulation of this process is associated with aberrant blood vessel formation (excessive or insufficient) in several human …
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Tumor-Cell Derived Inhibin, Beta A Is Essential For Adrenergic Effects On Fibroblast Activation
… Analysis and NetWalker were used to assess gene expression data from patients with known CESD (Center for Epidemiological Studies-Depression)-score alongside microdissected CAFs from primary ovarian cancer. Tumor samples from mice exposed to daily restraint-stress or non-stressed controls …
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The Role of Transforming Growth Factor-Beta In Alcohol-Induced Cardiac Fibrosis
… ethanol-induced fibroblast activation. </p> <p> Gene array analysis of ethanol-treated mice showed changes in extracellular matrix (ECM), inflammatory, oxidative stress, apoptotic, and lipid metabolism genes. Evidence of lipid accumulation and increased mast cell invasion was detected. An …
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T cell phenotyping of a mouse model of Activated PI3Kdelta syndrome
… gain-of-function mutation (E1021K) in the PIK3CD gene, encoding for the p110delta catalytic subunit of phosphoinositide 3-kinase (PI3K). APDS patients are lymphopenic, suffer from sinopulmonary infections and from increased susceptibility to bacterial and herpes group virus infections. Following T …
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Dendritic Cells in Normal and Inflamed Human Skin
… expressed these mature antigens, stimulated allogeneic T cells, and increased immunostimulatory capacity after the addition of TNF, PGE2, IL-1β, and IL-6. Functional studies were not performed on BDCA-3+ cells because of limited cell numbers. In normal dermis there also exists a large population …
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Analysis of the Role of Piwil2 gene in Tumorigenesis and Germline Stem Cell Metabolisms
Die Gene der piwi Familie werden durch ihre konservierte PAZ und Piwi Domäne charakterisiert und spielen eine wichtige Rolle bei der Stammzellselbsterneuerung , dem RNA silencing und der Translationsregulation in verschiedenen Organismen. Murine und humane Piwil2 Gene, Mitglieder der piwi …
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