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Showing 1 to 20 of 584 for “"Gel Electrophoresis"”.
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Gel electrophoresis for elemental speciation purposes
This work has shown that gel electrophoresis is a relevant fractionation method for trace elements bound to or complexed by macromolecules. Furthermore, detection methods of the trace elements that do not rely on the use of radiotracers were applied, making this combination relevant for materials …
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Characterization of Labeled Histones of Vicia Faba by Gel Electrophoresis
Made available in DSpace on 2014-12-09T22:25:46Z (GMT). No. of bitstreams: 1 6711856.pdf: 4448482 bytes, checksum: 47ede6e0d6d4105eb687de91216872c1 (MD5) Previous issue date: 1967
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Polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinaemia (Type III hyperlipidaemia)
Accurate phenotypic diagnosis of this disorder has generally relied on the quantitative analysis of centrifugally isolated VLDL. Analysis of VLDL chemical composition is not widely available due to the expense of acquiring ultracentrifuges and the highly-skilled staff necessary to perform the …
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Ruminal degradability of subfractions of protein sources as determined by gel electrophoresis
… for corn gluten meal to 72.3% for soybean meal. Gel electrophoresis was used to monitor rates of degradation in the rumen of fractions of corn gluten (CGM), CORN, cottonseed (CSM), peanut (PM), and soybean meal (SBM) protein fractions. Fractional degradation rates in the rumen were determined …
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The Role of the Voltage Gradient in the Agarose Gel Electrophoresis of DNA
… for predicting DNA mobility during agarose gel electrophoresis (AGE) from voltage gradient are developed from the data of McDonnel (36) for electrophoresis in a 1.6% agarose gel. These equations represented the data well for DNA between 2 and 10 kilobase pairs (KBp) in length. A computer …
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Horizontal starch gel electrophoresis as a technique for examining gray squirrel population genetics
The use of horizontal starch gel electrophoresis to separate gray squirrel blood serum was investigated. Three sources of variation in the techniques were identified: (1) minor variation within-run; (2) variation across-run; (3) variation due to the sampling technique. The frequencies of the most …
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… solve this problem is to use denaturing gradient gel electrophoresis (DGGE) to separate the PCR products before DNA sequencing. Denaturing gradient gel electrophoresis (DGGE) separates DNA molecules based on their melting point, which is determined by their DNA sequence. This analytical technique …
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A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil
… chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) procedure for the detection of fungal 18S ribosomal RNA gene was developed with reference cultures. Five AMF species were procured from the International Culture Collection of Arbuscular and Vesicular-Arbuscular …
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The use of alkaline gel electrophoresis to analyze hydrogen peroxide-caused DNA damage and repair in Escherichia coli
… needed. Accordingly, a method based on alkaline gel electrophoresis of DNA was developed and tested with isogenic strains of Escherichia coli deficient in one or more DNA repair enzymes, viz., recA (recA), exonuclease III (xthA), DNA polymerase I (po/A) or DNA polymerase I plus exonuclease I] …
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Single cell gel electrophoresis-based investigations of UVR- and visible light-induced single strand breakage in cultured human cells
… (3 mW cmˉ² ) were measured by single cell gel electrophoresis. The normal responses of 6 cell lines to the low fluence radiation show a peak in single strand breaks after approximately 2 minutes, after which time the single strand breakage returns to background levels despite continued …
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
… after 7 days exposure to BrdU. Two-dimensional gel electrophoresis was used to investigate BrdU specific changes in the proteome of DLKP BrdU treated and control cells. Cy3-labeled DLKP control were combined with Cy-5 labeled BrdU DLKP treated proteins and separated on the same 2-D gel along …
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Pulsed-field gel electrophoresis subtyping of salmonella isolates from carcasses, lymph nodes and fecal samples of cattle from slaughter facilities in Mexico
Salmonella is one of the leading causes of foodborne illnesses worldwide; however, in developing countries few surveillance programs exist, as a result limited information about the prevalence of this pathogen at pre-harvest is known. In order to identify the relationship between particular …
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Characterization of prokaryotic diversity in a chlorinated solvent-contaminated bedrock aquifer using denaturing gradient gel electrophoresis, ribosomal DNA sequencing, and real time PCR
… were generated by the use of denaturing gradient gel electrophoresis (DGGE), and were analyzed to detect spatial and seasonal changes. DGGE fingerprints revealed significant compositional differences between the attached and the unattached microbial communities. Cluster analysis suggested that the …
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Detection of Unique Tumor-Associated Proteins in the Urine of Patients with Transitional Cell Carcinoma by High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis
<p>A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer …
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Capillary and microchip gel electrophoresis using multiplexed fluorescence detection with both time-resolved and spectral-discrimination capabilities: applications in DNA sequencing using near-infrared fluorescence
… data during capillary and microchip electrophoresis. The utility of this hybrid fluorescence detector has been demonstrated by applying it to DNA sequencing applications. Coupling color discrimination with time-resolved fluorescence offers increased multiplexing capabilities because …
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Evaluating delivery of a monoclonal antibody using a linear Lorentz-force actuated needle-free injector
… enzyme-linked immunosorbent assay (ELISA) and gel electrophoresis methods. Using the ELISA method, we compared the ability of the antibody to bind to its specific antigen, HBsAg, both before and after ejection at multiple speeds. Changes in molecular size and charge of the monoclonal antibodies …
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The purification of C₁ components from the cellulase complex of Trichoderma viride
… on a weak anion exchange column (Bio-Gel DM). Elution with a pH 4.8 sodium citrate buffer resolved three cellulase fractions as well as a noncellulolytic fraction which remained on the column. The first peak of the elution pattern contained 97 per cent of the recovered C<sub>x</sub> …
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On-chip multi-dimensional biomolecule separation using multi-layer microfabricated valves
… of traditional two dimensional (2D) protein gel electrophoresis. Methods are described to achieve multi-dimensional biomolecule separation in a microfluidic channel. The new methods couple isoelectric focusing (IEF) with high ionic strength capillary electrophoresis (CE) by active micro valve …
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An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram
… (1977) did an early study using two-dimensional gel electrophoresis to determine the effects of various teratogens on whole embryos. No protein changes were found and that line of research was not continued. In this study two-dimensional gel electrophoresis is extensively used, in the belief that …
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An integrated geochemical and microbiological investigation of sulphate reduction in hypersaline pans
… community was studied by denaturing gradient gel electrophoresis, from which bands were excised for DNA sequencing. Denaturing gradient gel electrophoresis patterns were analysed statistically by cluster analysis and principal components analysis. High in situ rates of sulphate reduction (up …
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