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Showing 1 to 8 of 8 for “"GdnHCl"”.
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Investigation of the interaction of GdnHCl with poly-L-lysine, horseradish peroxidase and cytochrome c monitored via FTIR, CD and fluorescence spectroscopies
… probes were utilized to study the interaction of GdnHCl with three model systems: poly-L-lysine (PLL), horseradish peroxidase (HRP) and cytochrome c. Probing the chemically denatured states of proteins by FTIR is a novel technique, which has only recently been reported. To aid in the …
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Characterization of the unfolding, refolding, and aggregation pathways of two protein implicated in cataractogenesis : human gamma D and human gamma S crystallin
… as a function of guanidinium hydrochloride (GdnHCl) concentration at neutral pH and 37⁰C, using intrinsic tryptophan fluorescence to monitor in vitro folding. Equilibrium unfolding and refolding experiments with GdnHCl showed unfolded protein is more fluorescent than its native counter-part …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… measured histidine-heme loop formation in 3 M gdnHCl. A series of thirteen variants that include the A83S/A87S substitutions have been engineered with single surface histidine substitutions to provide a range of His-heme loop sizes from 10 to 111 residues and to provide direct comparison to …
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THERMODYNAMIC AND KINETIC PROPERTIES OF CYTOCHROMES C AND C’ IN THE DENATURED STATE: PROPENSITY FOR RESIDUAL STRUCTURE
… measured histidine-heme loop formation in 3 M gdnHCl. A series of thirteen variants that include the A83S/A87S substitutions have been engineered with single surface histidine substitutions to provide a range of His-heme loop sizes from 10 to 111 residues and to provide direct comparison to …
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In vitro interactions of the small heat shock protein chaperone human [alpha]B-crystallin with its physiological substrates in the lens [gamma]-crystallins
… high concentrations of guanidine hydrochloride (GdnHCl). However, in buffer or very low denaturant concentrations (< 1 M GdnHCl) aggregation of refolding [gamma]-crystallin intermediates competes with productive refolding. Diluting unfolded [gamma]C-, [gamma]D-, or [gamma]S-Crys to low GdnHCl …
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Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
… content, but were equally stable to GdnHCl denaturation when compared to rHDL containing wild-type proapoA-I. The reactivity with LCAT was reduced by 5-fold.
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The characterization of human [gamma]D-crystallin mutants and their differential interactions with the lens chaperone [alpha]B-crystallin
… with the refolding pathway after dilution from GdnHCl were indistinguishable between WT and mutant proteins. However, dramatic differences among the mutants were observed under different conditions, including physiological conditions in the absence of denaturant and partially destabilizing …
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Übertragung von BSE auf nicht humane Primaten als Modell für die variante Creutzfeldt-Jakob Erkrankung (vCJD) im Menschen
Die EU-Studie QLK1-CT-2002-01096 soll im Tiermodell das Risiko für den Menschen ermitteln, durch den Konsum von BSE-kontaminierten Nahrungsmitteln, an der varianten Creutzfeldt-Jakob Erkrankung (vCJD) zu erkranken. Als Tiermodell für den Menschen dienen Javaneraffen (Macaca fascicularis). In der …