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Showing 1 to 17 of 17 for “"Gcn4"”.
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Coordinated of Post-Translational Modifications of Yeast Transcriptional Activator Gcn4
Gcn4 is a transcriptional activator that regulates amino acid biosynthetic genes in yeast. Gcn4 is expressed under amino acid starvation conditions, and its degradation depends on phosphorylation, by either Pho85 or Cdk8, which triggers its ubiquitination and subsequent proteolysis by 26S …
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Gezielte Modifikation sowie Analyse der Bindungseigenschaften des Histidin Bindeproteins aus Escherichia coli und des GCN4 Leucinzippers aus Saccharomyces cerevisiae
… der Proteins für den jeweiligen Liganden. GCN4, ein allgemeiner Transkriptionsaktivator aus der Hefe, bildet stabile Homodimere durch hydrophobe Wechselwirkungen zwischen Leucinresten an jeder siebten Position. Austausch eines oder zweier dieser Leucinreste gegen ein Glycin bzw. ein Alanin …
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Role of Gcn4p in nutrient-controlled gene expression in Saccharomyces cerevisiae
… Kontrolle der Aminosäure-Biosynthese" ein. Gcn4p, der Transkriptionsfaktor dieses Systems, aktiviert die Expression von über 50 Genen, die in die verschiedenen Aminosäure-Biosynthesewege involviert sind. Im Rahmen dieser Arbeit konnte gezeigt werden, daß bei gleichzeitiger Limitierung von …
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Structural specificity in coiled coils : a and d position polar residues
… and the d positions of a reference coiled coil, GCN4-pVL. The reference coiled coil is very stable in solution and exists as a mixture of dimers and trimers. The placement of single polar residues in the otherwise hydrophobic core of GCN4-pVL has dramatic effects on both stability and oligomeric …
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Improving Thermodynamic Models of Transcription by Combining ChIP and Expression Measurements of Synthetic Promoters
… Additionally, the occupancy data suggests that Gcn4 binds in a cooperative manner and that Gcn4 occupancy is adversely affected by the presence of a nearby Nrg1 site. Finally, the occupancy data and expression data taken together suggest that Gcn4 binds in competition with another transcription …
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The specificity and evolution of gene regulatory elements
… to millions of sequences. I apply this method to GCN4, a yeast transcription factor, and reconstruct all known properties of its binding preferences. Additionally, I discover some new subtleties in its specificity and estimate dissociation constants for hundreds of thousands of sequences. I verify …
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Elucidation of the molecular pathways of lifespan extension by dietary restriction in yeast
… clustering to show afg3∆ was within the Gcn4 pathway of lifespan extension, and finally bring nuclear tRNA and Gln3 into the light- showing that these could positively affect lifespan without reducing translation.
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Interruptores moleculares basados en azobencenos y ciclodextrinas : aplicación en sistemas miméticos de factores de transcripción
… de miniproteínas del factor de transcripción GCN4 mediada por el agregado de un receptor alostérico y evaluado la interacción con diferentes ds ADN. Inicialmente, para este fin se sintetizó y caracterizo exhaustivamente un dímero fotomodulable de azo ß-ciclodextrina (4), el mismo se encuentra …
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Design and production of a candidate universal influenza A vaccine in Nicotiana benthamiana plants
… zipper derived from the General Control Protein (GCN4) was also included in some of the constructs to promote particle formation. In total, six different M2e-HA fusions were created: 5xM2e-GCN4-HAstalk, 5xM2e-GCN4-HAtrans, 5xM2e-HAstalk, 5xM2e-HAtrans, 1xM2e-HAstalk and 1xM2e-HAtrans. The …
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Functions of The Trna Splicing Endonuclease and Other Adventures In Rna Processing
… other essential function of TSEN triggers the Gcn4 response. This response is protective in our sen2 mutant and when Dbr1 is mutated in addition to sen2, the Gcn4 response is reduced as TSEN now has no competition for substrate to perform its essential function. As TSEN is involved in mRNA …
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The tyrosine regulated DAHP synthase and the biosynthetic pathway of aromatic amino acids in Saccharomyces cerevisiae
… Kontrolle der Aminosäurebiosynthese ist (ARO7c, gcn4), ist im Wachstum beeinträchtigt, wenn Phenylalanin ins Kultivierungsmedium gegeben worden ist. Dieser sogenannte Phe-Effekt kann unterdrückt werden, wenn der durch TRP2/TRP3 kodierte Anthranilat-Synthase-Komplex zu Beginn des Tryptophan-Wegs …
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HIV-1 Immune Escape and Neutralizing Antibodies : Solid Phase Proteoliposomes Containing HIV-1 Envelope Glycoproteins as Antigens and Immunogens and HIV-1 Core Envelope Glycoproteins Deficient in T-Helper Epitopes
… glycoprotein formulation, soluble YU2 gp140-GCN4 constructs, were also shown to better elicit broadly neutralizing antibodies in rabbits, extending a previous study in mice. These data support the hypothesis that trimeric envelope glycoprotein formulations are an advance over gp120-based …
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Molekulare Mechanismen der transkriptionellen Aktivierung durch die Transkriptionsfaktoren c-Jun und v-Jun
… zu den sauren Transkriptionsfaktoren VP16, GCN4 und Hap4, in S.cerevisiae unabhängig vom SAGA- und Ada-Komplex ist. Weder die Funktion des SAGA-Komplexes Histone zu acetylieren, noch seine Funktion als Brückenprotein sind für die transkriptionelle Aktivierung durch v-Jun notwendig. Obwohl …
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Regulation of The Candida Albicans Arginine Biosynthetic Pathway
… general amino acid control response, and that Gcn4, the master regulator of this response in <em>S. cerevisiae</em>, is a negative regulator of the <em>ARG</em> genes in <em>C. albicans</em>. <em>C. albicans</em> also has homologs for some components of the<em> S. cerevisiae </em>ArgR/Mcm1 …
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The response of eIF2α kinases to plant virus infection
… and yeast general control non-derepressible-4 (GCN4) uORFs were used. The uORFs did not regulate the translation of a main reporter ORF in Nicotiana benthamiana plants, regardless of the eIF2α phosphorylation status. In mammals, viral double-stranded RNA activates PKR. There is some evidence to …
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Regulation of gene expression and adhesion in <i>Saccharomyces cerevisiae</i>
… Allgemeinen Kontrolle der Aminosäurebiosynthese` Gcn4p. Um spezifisch regulierte Gene zu identifizieren, wurde Adhäsion durch Aminosäuremangel induziert und eine genomweite Transkriptionsanalyse von ∑1278b Hefezellen durchgeführt. 22 neue Gene waren durch Aminosäuremangel induzierbar. 72 Gene aus …
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Energy Landscaping - On the Relationship between Functionality and Sequence Mutations for Multifunctional Biomolecules
The process of protein and RNA folding has been understood in general terms through the principle of minimal frustration, and is usually thought of as being guided by a folding funnel on the energy landscape, which is based around the native structure. However, more recently, various biomolecules …