Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Gasdermin"”.
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Nuclear translocation of Gasdermin D (GSDMD) sensitizes chemotherapy through inhibiting PARP-1 in colorectal cancer
Gasdermin D (GSDMD) has recently been identified to play a central role in pyroptosis of monocytes, through its N-terminal effector domain cleaved by activated inflammatory caspases to form pores on cell membrane. However, as the universally expressed protein in cancer cells, its function beyond …
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Entamoeba histolytica-induced Activation of Caspase-4 Regulates Gasdermin D Cleavage to Mediate IL-1β Secretion in Macrophages
The parasite Entamoeba histolytica (Eh) is named for its capability to lyse healthy host tissues. A hallmark of Eh invasion in the gut is acute intestinal inflammation dominated by the secretion of pro-inflammatory cytokines. Eh in direct contact with macrophages activates caspase-1 by the assembly …
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NONCANONICAL PYROPTOSIS PROMOTES NONALCOHOLIC STEATOHEPATITIS VIA LIPID PEROXIDATION AND TRAINED IMMUNITY
… the newly discovered noncanonical (caspase-11/-Gasdermin-Ddependent) pathway has not been defined. Diet-induced NAFLD promoted hepatic steatosis and lobular inflammation in male WT mice. Caspase-11 deficiency decreases macrovesicular steatosis and total NAFLD Activity Score (NAS). High fat …
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Atypical Mechanisms of Synthesis, Activation and Release of Interleukin-1β from Vascular Smooth Muscle Cells
… VSMCs. VSMCs expressed the pore-forming protein Gasdermin D, and the active form of Gasdermin D permeabilised VSMCs, yet mature IL-1β was still not released. Finally, mature IL-1β extracted from VSMCs has 13-fold lower activity than equal amounts of mature IL-1β from Thp1 cells, however the …
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Biochemical Characterization of IpaH E3 Ubiquitin Ligase Effector Proteins and Their Host Substrates
… to identify the host substrate of IpaH7.8, Gasdermin B (GSDMB). GSDMB belongs to a large family of pore forming cytolysins that execute inflammatory cell death programs. While genetic studies have linked GSDMB polymorphisms to inflammatory disease, its function in human physiology remains …
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OROPOUCHE VIRUS DISRUPTION OF HOST CELL FUNCTIONS: PYROPTOSIS INDUCTION AND TRANSCRIPTIONAL SUPPRESSION
… -3, and -8, leading to the cleavage of Gasdermin D (GSDMD) and Gasdermin E (GSDME), and the release of IL-1β. Furthermore, NLRP3-deficient macrophages fail to activate caspases, cleave Gasdermins, or produce IL-1β upon infection. These results indicate that OROV infection triggers …
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The effects of E-cigarettes on pulmonary inflammation and inflammasome activation
… of capsase-1, interleukins 1ȕ and 18, along with gasdermin D, leading to inflammatory cell death called pyroptosis. As E-vapour has been shown to have pro-inflammatory effects on pulmonary cells, it is possible that vapours or vapour components could lead to inflammasome activation.<br/><br/>The …
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Preclinical Evaluation of Immunomodulatory Effects of Aurora Kinase Inhibition In Human Papillomavirus Positive Cancers
… in terms of increased levels of cleaved gasdermin E. Importantly, in both mouse and human HPV+ cancer cell lines treatment with alisertib, relative to vehicle control, resulted in significantly higher cell surface expression of calreticulin (CRT), and high mobility group box 1 protein …
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Mechanisms of Immunogenic Cell Death in Tumour Cells Infected with Oncolytic Vaccinia Virus
… Evidence was however found for active caspase-3, gasdermin E-mediated pyroptosis in one cancer cell line. Several vaccinia genes have been shown to function in blocking different host cell death pathways. We identified another inhibitor candidate, *A47L*, and tested its function as a regulator of …
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The role of IFN-γ in cell-autonomous immune responses against Salmonella Typhimurium and Toxoplasma gondii
… suggest that, in addition to the Caspase-4-Gasdermin-D axis, the role of IFN-γ-induced genes in the cell death pathway triggered by cytosolic LPS remains to be investigated. This thesis also examined the role of IFN-γ in response to infection by the eukaryotic parasite Toxoplasma gondii, an …
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Roles of Cytosolic Nucleic Acid Sensors in Cancer and Infection
… the protective AIM2 inflammasome responses. Gasdermin D (GSDMD) is activated by caspase-1 to generate pores on the plasma membrane to induce pyroptosis downstream of the AIM2 inflammasome. We also demonstrated that mice lacking GSDMD were highly susceptible to <em>F. novicida </em>infection. …
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Caspase isoforms in inflammasome activation
… pro- IL-18 to their active form and cleavage of gasdermin D to induce pyroptosis. Caspase-1 is the main inflammatory caspase responsible for cytokine maturation and induction of cell death, while human caspase-4, -5 and the mouse orthologue caspase-11 play an essential role in cytosolic bacterial …
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… an increase in caspase-3 activation and gasdermin E (GSDME) cleavage in WT EMCV-infected samples relative to infection with 2B\*KO EMCV prior to 24 hpi. Work with caspase KO cell lines confirmed that EMCV-induced rapid cell lysis is supported by caspase-3 but that WT EMCV can also induce …
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Epigenetic regulation of gene expression by BRD4 in cancer and innate immune response
… Asc oligomerization, IL-1 maturation, gasdermin-D (Gsdmd) cleavage, and pyroptosis upon bacterial infection. RNA-seq results unveiled that the ablation of Brd4 in BMDMs suppressed the transcription of Naips and Nlrc4. Mechanistically, ChIP-seq analysis revealed that Brd4 co-localized …