Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Galactosyltransferase"”.
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Identification and Function of Beta-1,4-Galactosyltransferase Associated Proteins
In summary, a pig testis phage display cDNA library screening and protein affinity chromatography were applied to identify GalTase-I associated proteins. Several proteins were identified as candidates that interact with GalTase-I and potentially play important roles during fertilization. …
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Synthese und enzymatische Umsetzung modifizierter Donorsubstrate mit dem Enzym beta(1,4)Galactosyltransferase
… die Enzym-Substrat-Wechselwirkungen der beta-1,4-Galactosyltransferase des Leloir-Weges zu verstehen, ist in vielen Arbeiten die Substratspezifität untersucht worden. Ziel dieser Arbeit war die Synthese von Zuckernucleotiden als Donorsubstrate mit fluormodifizierten Ribosebausteinen und deren …
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Molecular Characterization and Inhibition of Antibodies Elicited Against Galactosyltransferase Knockout Pig Xenografts
<p>On average, 21 people in the United States die every day while waiting for a transplantable organ. Moreover, at the end of 2014, nearly 130,000 additional individuals were still on the transplant waiting list. Xenotransplantation, the transplantation of cells and/or organs from a member of …
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Investigation of galactosyltransferase and α-lactalbumin-like proteins in mammalian reproductive tracts / by Yulu Tang
Studies the functions of galactosyltransferase (GalTase) and b-lactalbumin (b-lac) in mammalian reproduction. Investigates whether GalTase and b-lac-like proteins are widely present in epididymal plasma of various mammalian species and their function in reproduction; whether GalTase is also present …
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[alpha](1,3)galactosyltransferase mediated gene therapy for cancer: developing new models and improving on existing ones
<p>Alpha (1,3)galactosyltransferase is the major xenoantigen associated with hyperacute rejection of xenotransplants. Tumor vaccines engineered to express this gene may show promise in breaking tumor tolerance. However there has been a lack of alternative tumor models to the highly published B16 …
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Untersuchung von cosmc-Mutationen bei Patienten mit IgA-Nephropathie
… most common glomerulonephritis in the world. The galactosyltransferase core-1 beta3-galactosyltransferase-1 (C1GALT1) and its chaperone cosmc are specifically required for O-galactosylation of the IgA1 hinge region. Mutations in the cosmc gene result in a secondary loss of function of C1GALT1 with …
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Assays for lycosyltransferases
… <br/>main focus was on the bacterial GT, α1,4-galactosyltransferase C (LgtC) which transfers galactose from uridine-diphosphate-galactose to the terminal lactose on the cell wall lipooligopolysacharide of Neisseria meningitidis.<br/>A fluorescence-based ligand displacement assay (LDA) was …
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The kinetics of endosome processing
… mouse macrophage-cells, P388D₁, as catalysed by galactosyltransferase. This provided a general membrane marker. After endocytosis-derived redistribution of membrane marker between the cell surface and endosomal membrane, a steady state was established with about 16% of the label on internal …
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Role of VimF in Gingipain Maturation in Porphyromonas gingivalis
… results suggest that VimF glycoprotein is a galactosyltransferase that may be specific for gingipain glycosylation.
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Chemical tools to probe carbohydrate recognition and glycoconjugate biosynthesis
… lipoarabinomannan in Mycobacterium smegmatis and galactosyltransferase activities involved in the decoration of GPI anchor in Trypanosoma brucei. Further application of this methodology allowed detection and characterisation of mannosyltransferase activities responsible for biosynthesis of …
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Interleukin-15 and [alpha](1,3)galactosyl-expressing vaccine combination therapy for melanoma
… was previously studied using the alpha1,3-galactosyltransferase knockout (alphaGT KO) mouse model and B16 melanoma cells genetically modified to express alphaGal epitopes (B16alphaGal). In this model, the B16alphaGal vaccine showed efficacy in treating pre-existing subcutaneous and …
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Elucidating the role of TM9SF2 in membrane trafficking
… novel TM9SF2 cargo proteins, the enzymes, β-1,4- galactosyltransferase 1 (B4GALT1) and Sulfhydryl oxidase 2 (QSOX2). We show that two residues within the transmembrane domain of B4GALT1 are required for interaction with TM9SF2. Furthermore, as QSOX1, a paralogue of QSOX2, plays a role in …
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Slc39a8/zip8 Influences Complex Traits By Regulating Metal Ion Metabolism
… activity of a Mn-dependent enzyme, β-1, 4-galactosyltransferase, while homozygosity of a SLC39A8 loss-of-function variant is associated with hypogalactosylation, suggesting that protein N-glycosylation may be related to the association of SLC39A8 with complex traits in GWAS. Slc39a8 acts …
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Sequence-structure-function relationships of glycosyltransferases in families GT43, GT47, and GT64
… and a homologous xylan galactosyltransferase, I was nevertheless successful in identifying a novel xyloglucan-specific enzyme from Coffea canephora on the basis of its protein sequence. As a whole, the results in this thesis provide insight into the relationship between …
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Entwicklung und Optimierung einer allgemeinen Strategie zur Synthese von multiantennären N-Glycanen des „bisecting“-Typs
… Verlängerung des bisecting-GlcNAc durch Galactosyltransferase nur dann erfolgen kann, wenn kein alpha-1,6-Arm am N-Glycan vorhanden ist.
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Modifying N-linked glycosylation in tobacco to produce human-type post-translational modifications
… IV and V (GNTIV and GNTV), β-1,4-galactosyltransferase (B4GALT1), and α-2,6-sialyltransferase (ST6GAL) need to be expressed in plant cells. As glycosylation is a stepwise process, enzymes need to localise to either medial or trans-Golgi body cisternae. For this, a protein targeting …
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Genetic studies of candidate genes in the glycoalkaloid biosynthetic pathway of potato
… HMG2); 2,3-squalene epoxidase (SQE); solanidine galactosyltransferase (SGT1); and solanidine glucosyltransferase (SGT2)]. A germplasm panel of six wild potato species [Solanum chacoense (chc 80-1), S. commersonii subsp. commersonii, S. demissum, S. sparsipilum, S. spegazzinii, and S. …
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Defining the role of cellulose synthase-like M genes in the plant cell wall
… indicate that tomato CslM2a may be a putative galactosyltransferase in SGA biosynthesis, in addition to its established role as a glucuronosyltransferase in the triterpenoid saponin biosynthesis pathway. The work in this thesis confirms the unusual dual-function of members of this cellulose …
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Investigation of Putative Genetic Factors Associated with Soybean [Glycine Max (L.) Merr.] Seed Quality Traits
… gene mapping approach. A predicted soybean galactosyltransferase gene was established as a candidate gene due to its observed segregation with the single low stachyose QTL observed on molecular linkage group (MLG) C2 in both populations. Sequencing of this putative gene revealed a unique 3 …
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