Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"GUV"”.
-
A Bottom-Up Approach to Building an Artificial Cell by Self-Assembly
… the glycolipid is fully dispersed in the GUV membrane. At concentrations above 10%, the formation of lipid tubules was observed, consistent with the formation of a columnar lipid phase. Lipid tubules are observed in aqueous and oil solvents, suggesting that both hexagonal and inverted …
-
Mechanisms of Multivesicular Body Biogenesis and Exosome Release
… die Freisetzung von Exosomen. Daher wurden GUVs (giant unilamelar vesicles) mit Sphingomyelin hergestellt, in denen zwei unterschiedlichen Phasen vorlagen. Die Hydrolyse von Sphingomyelin an der äußeren Schicht der GUV bewirkte eine Abschnürung intraluminaler Vesikel aus der gelartigen …
-
Quantification of membrane transport rates using optofluidics
… microfluidic Giant Unilamellar lipid Vesicle (GUV) formation method – is refined by the integration with a novel Pinched Flow Fractionation (PFF) purification module. PFF removes formationassociated residues at a range of length-scales, from surfactant molecules to oil droplets, with high …
-
A Lab-on-a-chip System for the Standardised Characterisation of Membrane Active Antimicrobials
… standardised manner. As a result, I present the "GUV Studio", a bespoke multilayer microfluidic platform to quantify membranolytic efficacy and characterise the mode of action of AMPs. The platform is a biomimetic vesicle-based screening assay, which integrates an element for the high-throughput …
-
Electro-deformation of a moving boundary: a drop interface and a lipid bilayer membrane
… deformation of a giant unilamellar vesicle (GUV) under an alternating current (AC) electric field. The vesicle membrane is modeled as a thin capacitive spheroidal shell and the equilibrium vesicle shape is computed from balancing the mechanical forces between the fluid, the membrane and the …
-
Efficient coarse-grained brownian dynamics simulations for dna and lipid bilayer membrane with hydrodynamic interactions
… systems such as a giant unilamellar vesicle (GUV) and RBCs. Moreover, this work also considers the effect of cytoskeleton on the lipid membrane dynamics as a model for RBC dynamics, and incorporates coarse-grained water molecules to account for hydrodynamic interactions. (3) An action field …
-
Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
-
Reconstituting APP and BACE in proteoliposomes to characterize lipid requirements for β-secretase activity
… in large (~100nm, LUVs) and giant (10-150microm, GUVs) unilamellar vesicles. Using a soluble peptide substrate mimicking the beta-cleavage site of APP, we have examined the involvement of individual lipid species in modulating BACE activity in LUVs of various lipid compositions. We have identified …